The Journal of Cell Biology, Vol 65, 428-438, Copyright © 1975 by The Rockefeller University Press
Isolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation
LJ Romrell, MR Coppe, DR Munro and S Ito
Methods of tissue dissociation and cell separation have been modified to
obtain highly enriched fractions of mouse gastric parietal cells.
Suspension of gastric mucosal cells are prepared by pronase digestion of
the glandular portion of the stomach from adult mice. By utilizing the
velocity sedimentation technique to separate cells of different sizes it is
possible to recovery parietal cells, which are larger than the other cell
types, in fractions with purity of 75-95%. The homogeneity of cell
fractions has been assessed by light and electron microscopy. The ability
of the isolated cells to exclude the dye trypan blue, to incorporate
labeled substrate, to consume oxygen, and to retain their structural
integrity indicates that they are viable and still capable of functional
activity.
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