BALD-2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii

doi:10.1083/jcb.66.3.480

This Article

	Full Text (PDF, 3060K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Goodenough, U. W.
	Articles by St.Clair, H. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Goodenough, U. W.
	Articles by St.Clair, H. S.


Social Bookmarking

	What's this?

ARTICLES

BALD-2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii

UW Goodenough and HS St.Clair

The mutant strain bald-2 is unique among "flagellaless" strains of Chlamydomonas reinhardtii isolated to date, in that it possesses a mutant basal body: it is only capable of forming a ring of nine singlet microtubules, 180 nm in diameter, instead of the usual triplet basal body which is 225 nm in diameter. This singlet basal body lacks structural stability and the ability to associate with striated fiber material but retains two critical properties of basal bodies, namely, information specifying the length to which it should elongate and the ability to induce, albeit rarely, a flagellar transition region, a short, singlet-containing axoneme, and a specialized tunnel in the cell wall through which flagella normally emerge. The mutation seems to be specific for B- and C-microtubule synthesis or assembly since all other cytoplasmic sets of microtubules appear normal in numbers, orientation, and stability.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Keller, L. C., Geimer, S., Romijn, E., Yates, J. III, Zamora, I., Marshall, W. F. (2009). Molecular Architecture of the Centriole Proteome: The Conserved WD40 Domain Protein POC1 Is Required for Centriole Duplication and Length Control. Mol. Biol. Cell 20: 1150-1166 [Abstract] [Full Text]
Hoffmann, X.-K., Beck, C. F. (2005). Mating-Induced Shedding of Cell Walls, Removal of Walls from Vegetative Cells, and Osmotic Stress Induce Presumed Cell Wall Genes in Chlamydomonas. Plant Physiol. 139: 999-1014 [Abstract] [Full Text]
Matsuura, K., Lefebvre, P. A., Kamiya, R., Hirono, M. (2004). Bld10p, a novel protein essential for basal body assembly in Chlamydomonas: localization to the cartwheel, the first ninefold symmetrical structure appearing during assembly. JCB 165: 663-671 [Abstract] [Full Text]
O'Toole, E. T., Giddings, T. H., McIntosh, J. R., Dutcher, S. K. (2003). Three-dimensional Organization of Basal Bodies from Wild-Type and {delta}-Tubulin Deletion Strains of Chlamydomonas reinhardtii. Mol. Biol. Cell 14: 2999-3012 [Abstract] [Full Text]
Dutcher, S. K., Morrissette, N. S., Preble, A. M., Rackley, C., Stanga, J. (2002). epsilon -Tubulin Is an Essential Component of the Centriole. Mol. Biol. Cell 13: 3859-3869 [Abstract] [Full Text]
Preble, A. M., Giddings, T. H. , Jr., Dutcher, S. K. (2001). Extragenic Bypass Suppressors of Mutations in the Essential Gene BLD2 Promote Assembly of Basal Bodies With Abnormal Microtubules in Chlamydomonas reinhardtii. Genetics 157: 163-181 [Abstract] [Full Text]
Norrander, J. M., deCathelineau, A. M., Brown, J. A., Porter, M. E., Linck, R. W. (2000). The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas. Mol. Biol. Cell 11: 201-215 [Abstract] [Full Text]
Rosenbaum, J. L., Cole, D. G., Diener, D. R. (1999). Intraflagellar Transport: The Eyes Have It. JCB 144: 385-388 [Full Text]
Cole, D. G., Diener, D. R., Himelblau, A. L., Beech, P. L., Fuster, J. C., Rosenbaum, J. L. (1998). Chlamydomonas Kinesin-II-dependent Intraflagellar Transport (IFT): IFT Particles Contain Proteins Required for Ciliary Assembly in Caenorhabditis elegans Sensory Neurons. JCB 141: 993-1008 [Abstract] [Full Text]
Wilson, P., Fuller, M., Borisy, G. (1997). Monastral bipolar spindles: implications for dynamic centrosome organization. J. Cell Sci. 110: 451-464 [Abstract]