Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique

doi:10.1083/jcb.66.3.676

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Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique

FG Moser, BP Dorman and FH Ruddle

The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.
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