Initiation and growth of microtubules from mitotic centers in lysed mammalian cells

doi:10.1083/jcb.67.3.744

This Article

	Full Text (PDF, 4431K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Snyder, J. A.
	Articles by McIntosh, J. R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Snyder, J. A.
	Articles by McIntosh, J. R.


Social Bookmarking

	What's this?

ARTICLES

Initiation and growth of microtubules from mitotic centers in lysed mammalian cells

JA Snyder and JR McIntosh

Metaphase PtK1 cells, lysed into polymerization-competent microtubule protein, maintain a spindle which will gain or lose birefringence depending on the concentration of disassembled tubulin subunits used in the lysis medium. Concentrations of tubulin subunits greater than the equilibrium monomer value promote a rate and extent of birefringence increase that is proportional to the subunit concentration. Increase in spindle birefringence can be correlated with an increase in tubule number, though the relationship is not strictly linear. Increase in spindle tubule number is due to an vivo-like initiation of tubules at the mitotic centers, as well as tubulin addition onto pre-existing spindle fragments. Colcemid-treated prometaphase cells lysed into polymerization-competent tubulin develop large asters in the region of the centrioles and short tubules at kinetochores, making it unlikely that all microtubule formation in lysed cell preparations is dependent on tubulin addition to short tubule fragments. Asters can also form in colcemid-treated prometaphase cells lysed in tubulin that is incapable of spontaneous tubule initiation, suggesting that the centriolar region serves a tubule-initiator function in our lysed cell preparations. The ability of the centriole to initiate microtubule assembly is a time- dependent process-a ripening effect takes place between prophase and late prometaphase. Ripening is expressed by an increase in the number and length of tubules found associated with the centriolar region.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Sankaran, S., Crone, D. E., Palazzo, R. E., Parvin, J. D. (2007). Aurora-A Kinase Regulates Breast Cancer Associated Gene 1 Inhibition of Centrosome-Dependent Microtubule Nucleation. Cancer Res. 67: 11186-11194 [Abstract] [Full Text]
O'Connell, C. B., Khodjakov, A. L. (2007). Cooperative mechanisms of mitotic spindle formation. J. Cell Sci. 120: 1717-1722 [Abstract] [Full Text]
Uetake, Y., Loncarek, J., Nordberg, J. J., English, C. N., La Terra, S., Khodjakov, A., Sluder, G. (2007). Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cells. JCB 176: 173-182 [Abstract] [Full Text]
Maiato, H., Rieder, C. L., Khodjakov, A. (2004). Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis. JCB 167: 831-840 [Abstract] [Full Text]
Piehl, M., Tulu, U. S., Wadsworth, P., Cassimeris, L. (2004). Centrosome maturation: Measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1. Proc. Natl. Acad. Sci. USA 101: 1584-1588 [Abstract] [Full Text]
Heitz, M. J., Petersen, J., Valovin, S., Hagan, I. M. (2002). MTOC formation during mitotic exit in fission yeast. J. Cell Sci. 114: 4521-4532 [Abstract] [Full Text]
Takada, S., Shibata, T., Hiraoka, Y., Masuda, H. (2000). Identification of Ribonucleotide Reductase Protein R1 as an Activator of Microtubule Nucleation in Xenopus Egg Mitotic Extracts. Mol. Biol. Cell 11: 4173-4187 [Abstract] [Full Text]
Salisbury, J. L., Lingle, W. L., White, R. A., Cordes, L. E.M., Barrett, S. (1999). Microtubule Nucleating Capacity of Centrosomes in Tissue Sections. J. Histochem. Cytochem. 47: 1265-1274 [Abstract] [Full Text]
Khodjakov, A., Rieder, C. L. (1999). The Sudden Recruitment of {gamma}-Tubulin to the Centrosome at the Onset of Mitosis and Its Dynamic Exchange Throughout the Cell Cycle, Do Not Require Microtubules. JCB 146: 585-596 [Abstract] [Full Text]
Fry, A. M., Mayor, T., Meraldi, P., Stierhof, Y.-D., Tanaka, K., Nigg, E. A. (1998). C-Nap1, a Novel Centrosomal Coiled-Coil Protein and Candidate Substrate of the Cell Cycle-regulated Protein Kinase Nek2. JCB 141: 1563-1574 [Abstract] [Full Text]
Euteneuer, U, Graf, R, Kube-Granderath, E, Schliwa, M (1998). Dictyostelium gamma-tubulin: molecular characterization and ultrastructural localization. J. Cell Sci. 111: 405-412 [Abstract]
Tassin, A., Celati, C, Paintrand, M, Bornens, M (1997). Identification of an Spc110p-related protein in vertebrates. J. Cell Sci. 110: 2533-2545 [Abstract]
Masuda, H, Shibata, T (1996). Role of gamma-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body. J. Cell Sci. 109: 165-177 [Abstract]
Whitfield, W., Chaplin, M., Oegema, K, Parry, H, Glover, D. (1995). The 190 kDa centrosome-associated protein of Drosophila melanogaster contains four zinc finger motifs and binds to specific sites on polytene chromosomes. J. Cell Sci. 108: 3377-3387 [Abstract]
Dominguez, J., Buendia, B, Lopez-Otin, C, Antony, C, Karsenti, E, Avila, J (1994). A protein related to brain microtubule-associated protein MAP1B is a component of the mammalian centrosome. J. Cell Sci. 107: 601-611 [Abstract]
Errabolu, R, Sanders, M., Salisbury, J. (1994). Cloning of a cDNA encoding human centrin, an EF-hand protein of centrosomes and mitotic spindle poles. J. Cell Sci. 107: 9-16 [Abstract]
Wendell, K., Wilson, L, Jordan, M. (1993). Mitotic block in HeLa cells by vinblastine: ultrastructural changes in kinetochore-microtubule attachment and in centrosomes. J. Cell Sci. 104: 261-274 [Abstract]
McIntosh, J., Koonce, M. (1989). Mitosis. Science 246: 622-628 [Abstract]
Haimo, L. T., Telzer, B. R. (1982). Dynein-Microtubule Interactions: ATP-sensitive Dynein Binding and the Structural Polarity of Mitotic Microtubules. Cold Spring Harb Symp Quant Biol 46: 207-217 [Abstract]