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16th INTERNATIONAL VASCULAR BIOLOGY MEETING
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The Journal of Cell Biology, Vol 67, 887-894, Copyright © 1975 by The Rockefeller University Press

ARTICLES

Isolation of liver lysosomes by iron loading. Ultrastructural characterization

H Glaumann, H Jansson, B Arborgh and JL Ericsson

In summary, the data demonstrate that, by the use of repeated injections of an iron sorbitol complex, it is possible to isolate a fraction highly enriched in hydrolytic enzymes (60 times over the homogenate) and in well preserved lysosomes emanating almost entirely from liver parenchymal cells. The advantage of adding fixative to the bottom of the gradient and of using en bloc staining with uranyl acetate is also demonstrated.
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