Subcellular localization of B apoprotein of plasma lipoproteins in rat liver

doi:10.1083/jcb.69.2.241

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Subcellular localization of B apoprotein of plasma lipoproteins in rat liver

CA Alexander, RL Hamilton and RJ Havel

Multispecific antigen-binding fragments (Fab) from rabbit antisera against rat very low density lipoproteins (VLDL) and Fab against rat low density lipoproteins that were monospecific for the B apoprotein were conjugated to horseradish peroxidase. Conjugates were incubated with 6-mum frozen sections from fresh and perfusion-fixed livers and with tissue chopper sections (40 mum thick) from perfusion-fixed livers. In the light microscope, specific reaction product was present in all hepatocytes of experimental sections as intense brown to black spots whose locations corresponded to the distribution of the Golgi apparatus: along the bile canaliculi, near the nuclei, and between the nuclei and bile canaliculi. Perfusion fixation with formaldehyde produced satisfactory ultrastructural preservation with retention of lipoprotein antigenic determinants. In the electron microscope, patches of cisternae and ribosomes of the rough endoplasmic reticulum (ER) and particularly its smooth-surfaced ends, vesicles located between the rough ER and the Golgi apparatus, the Golgi apparatus and its secretory vesicles and VLDL particles in the space of Disse all bore reaction product. The tubules and vesicles of typical hepatocyte smooth ER did not contain reaction product, nor did the osmiophilic particles contained therin. The localization obtained in this study together with other evidence suggests a sequence for the biosynthesis of VLDL that differs in some respects from that proposed by others: (a) the triglyceride-rich particle originates in smooth ER where triglycerides are synthesized; (b) at the junction of the smooth and rough ER the particle receives apoproteins synthesized in the rough ER; (c) specialized tubules transport the particle, now a nascent lipoprotein, to the Golgi apparatus where concentration occurs in secretory vesicles; (d) secretory vesicles move to the sinusoidal surface where the particles are secreted into the space of Disse by fusion of the vesicular membrane with the plasma membrane of the hepatocyte.
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Hebbachi, A.-M., Gibbons, G. F. (2001). Microsomal membrane-associated apoB is the direct precursor of secreted VLDL in primary cultures of rat hepatocytes. J. Lipid Res. 42: 1609-1617 [Abstract] [Full Text]
Brown, A.-M., Gibbons, G. F. (2001). Insulin Inhibits the Maturation Phase of VLDL Assembly via a Phosphoinositide 3-Kinase--Mediated Event. Arterioscler. Thromb. Vasc. Bio. 21: 1656-1661 [Abstract] [Full Text]
Davis, R. A., Hui, T. Y. (2001). 2000 George Lyman Duff Memorial Lecture : Atherosclerosis Is a Liver Disease of the Heart. Arterioscler. Thromb. Vasc. Bio. 21: 887-898 [Abstract] [Full Text]
Lee, R. G., Willingham, M. C., Davis, M. A., Skinner, K. A., Rudel, L. L. (2000). Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates. J. Lipid Res. 41: 1991-2001 [Abstract] [Full Text]
Stillemark, P., Boren, J., Andersson, M., Larsson, T., Rustaeus, S., Karlsson, K.-A., Olofsson, S.-O. (2000). The Assembly and Secretion of Apolipoprotein B-48-containing Very Low Density Lipoproteins in McA-RH7777 Cells. J. Biol. Chem. 275: 10506-10513 [Abstract] [Full Text]
Zhao, Y., McCabe, J. B., Vance, J., Berthiaume, L. G. (2000). Palmitoylation of Apolipoprotein B Is Required for Proper Intracellular Sorting and Transport of Cholesteroyl Esters and Triglycerides. Mol. Biol. Cell 11: 721-734 [Abstract] [Full Text]
Xiao, Q., Elovson, J., Schumaker, V. N. (2000). Rat McA-RH7777 cells efficiently assemble rat apolipoprotein B-48 or larger fragments into VLDL but not human apolipoprotein B of any size. J. Lipid Res. 41: 116-125 [Abstract] [Full Text]
Mahley, R. W., Huang, Y., Rall, S. C. , Jr. (1999). Pathogenesis of type III hyperlipoproteinemia (dysbetalipoproteinemia): questions, quandaries, and paradoxes. J. Lipid Res. 40: 1933-1949 [Abstract] [Full Text]
Terasawa, Y., Cases, S. J., Wong, J. S., Jamil, H., Jothi, S., Traber, M. G., Packer, L., Gordon, D. A., Hamilton, R. L., Farese, R. V. , Jr. (1999). Apolipoprotein B-related gene expression and ultrastructural characteristics of lipoprotein secretion in mouse yolk sac during embryonic development. J. Lipid Res. 40: 1967-1977 [Abstract] [Full Text]
Hebbachi, A.-M., Brown, A.-M., Gibbons, G. F. (1999). Suppression of cytosolic triacylglycerol recruitment for very low density lipoprotein assembly by inactivation of microsomal triglyceride transfer protein results in a delayed removal of apoB-48 and apoB-100 from microsomal and Golgi membranes of primary rat hepatocytes. J. Lipid Res. 40: 1758-1768 [Abstract] [Full Text]
Wang, Y., Tran, K., Yao, Z. (1999). The Activity of Microsomal Triglyceride Transfer Protein Is Essential for Accumulation of Triglyceride within Microsomes in McA-RH7777 Cells. A UNIFIED MODEL FOR THE ASSEMBLY OF VERY LOW DENSITY LIPOPROTEINS. J. Biol. Chem. 274: 27793-27800 [Abstract] [Full Text]
Hamilton, R. L., Moorehouse, A., Lear, S. R., Wong, J. S., Erickson, S. K. (1999). A rapid calcium precipitation method of recovering large amounts of highly pure hepatocyte rough endoplasmic reticulum. J. Lipid Res. 40: 1140-1147 [Abstract] [Full Text]
Shelness, G. S., Ingram, M. F., Huang, X. F., DeLozier, J. A. (1999). Apolipoprotein B in the Rough Endoplasmic Reticulum: Translation, Translocation and the Initiation of Lipoprotein Assembly. J. Nutr. 129: 456-456 [Abstract] [Full Text]
Rustaeus, S., Lindberg, K., Stillemark, P., Claesson, C., Asp, L., Larsson, T., Boren, J., Olofsson, S.-O. (1999). Assembly of Very Low Density Lipoprotein: A Two-Step Process of Apolipoprotein B Core Lipidation. J. Nutr. 129: 463-463 [Abstract] [Full Text]
Walzem, R. L., Hansen, R. J., Williams, D. L., Hamilton, R. L. (1999). Estrogen Induction of VLDLy Assembly in Egg-Laying Hens. J. Nutr. 129: 467-467 [Abstract] [Full Text]
Hamilton, R. L., Wong, J. S., Cham, C. M., Nielsen, L. B., Young, S. G. (1998). Chylomicron-sized lipid particles are formed in the setting of apolipoprotein B deficiency. J. Lipid Res. 39: 1543-1557 [Abstract] [Full Text]
Du, X., Daniel Stoops, J., Mertz, J. R., Michael Stanley, C., Dixon, J. L. (1998). Identification of Two Regions in Apolipoprotein B100 that are Exposed on the Cytosolic Side of the Endoplasmic Reticulum Membrane. JCB 141: 585-599 [Abstract] [Full Text]
Huang, X. F., Shelness, G. S. (1997). Identification of Cysteine Pairs within the Amino-terminal 5% of Apolipoprotein B Essential for Hepatic Lipoprotein Assembly and Secretion. J. Biol. Chem. 272: 31872-31876 [Abstract] [Full Text]
Wang, Y., McLeod, R. S., Yao, Z. (1997). Normal Activity of Microsomal Triglyceride Transfer Protein Is Required for the Oleate-induced Secretion of Very Low Density Lipoproteins Containing Apolipoprotein B from McA-RH7777 Cells. J. Biol. Chem. 272: 12272-12278 [Abstract] [Full Text]
Davis, R. A. (1997). Evolution of Processes and Regulators of Lipoprotein Synthesis: From Birds to Mammals. J. Nutr. 127 : 795S-795S [Abstract] [Full Text]
Wu, X., Sakata, N., Lele, K. M., Zhou, M., Jiang, H., Ginsberg, H. N. (1997). A Two-site Model for ApoB Degradation in HepG2 Cells. J. Biol. Chem. 272: 11575-11580 [Abstract] [Full Text]
Ingram, M. F., Shelness, G. S. (1997). Folding of the Amino-terminal Domain of Apolipoprotein B Initiates Microsomal Triglyceride Transfer Protein-dependent Lipid Transfer to Nascent Very Low Density Lipoprotein. J. Biol. Chem. 272: 10279-10286 [Abstract] [Full Text]
Rusinol, A. E., Jamil, H., Vance, J. E. (1997). In Vitro Reconstitution of Assembly of Apolipoprotein B48-containing Lipoproteins. J. Biol. Chem. 272: 8019-8025 [Abstract] [Full Text]
Gordon, D. A., Jamil, H., Gregg, R. E., Olofsson, S.-O., Boren, J. (1996). Inhibition of the Microsomal Triglyceride Transfer Protein Blocks the First Step of Apolipoprotein B Lipoprotein Assembly but Not the Addition of Bulk Core Lipids in the Second Step. J. Biol. Chem. 271: 33047-33053 [Abstract] [Full Text]
Wu, X., Zhou, M., Huang, L.-S., Wetterau, J., Ginsberg, H. N. (1996). Demonstration of a Physical Interaction between Microsomal Triglyceride Transfer Protein and Apolipoprotein B during the Assembly of ApoB-containing Lipoproteins. J. Biol. Chem. 271: 10277-10281 [Abstract] [Full Text]
Innerarity, T. L., Bor�n, J., Yamanaka, S., Olofsson, S.-O. (1996). Biosynthesis of Apolipoprotein B48-containing Lipoproteins. J. Biol. Chem. 271: 2353-2356 [Full Text]
Rustaeus, S., Lindberg, K., Bor�n, J., Olofsson, S.-O. (1995). Brefeldin A Reversibly Inhibits the Assembly of ApoB Containing Lipoproteins in McA-RH7777 Cells. J. Biol. Chem. 270: 28879-28886 [Abstract] [Full Text]
Rusiol, A. E., Vance, J. E. (1995). Inhibition of Secretion of Truncated Apolipoproteins B by Monomethylethanolamine Is Independent of the Length of the Apolipoprotein. J. Biol. Chem. 270: 13318-13325 [Abstract] [Full Text]
Allan, V., Vale, R. (1994). Movement of membrane tubules along microtubules in vitro: evidence for specialised sites of motor attachment. J. Cell Sci. 107: 1885-1897 [Abstract]
Asp, L., Claesson, C., Boren, J., Olofsson, S.-O. (2000). ADP-ribosylation Factor 1 and Its Activation of Phospholipase D Are Important for the Assembly of Very Low Density Lipoproteins. J. Biol. Chem. 275: 26285-26292 [Abstract] [Full Text]
Bakillah, A., Hussain, M. M. (2001). Binding of Microsomal Triglyceride Transfer Protein to Lipids Results in Increased Affinity for Apolipoprotein B. EVIDENCE FOR STABLE MICROSOMAL MTP-LIPID COMPLEXES. J. Biol. Chem. 276: 31466-31473 [Abstract] [Full Text]
Tran, K., Wang, Y., DeLong, C. J., Cui, Z., Yao, Z. (2000). The Assembly of Very Low Density Lipoproteins in Rat Hepatoma McA-RH7777 Cells Is Inhibited by Phospholipase A2 Antagonists. J. Biol. Chem. 275: 25023-25030 [Abstract] [Full Text]