The Journal of Cell Biology, Vol 71, 89-95, Copyright © 1976 by The Rockefeller University Press

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Immunofluorescence evidence for the absence of histone H1 in a mitotically dividing, genetically inactive nucleus

CA Johmann and MA Gorovsky

Antibodies directed against whole histone and purified lysine-rich histone H1 extracted from isolated macronuclei of the ciliate Tetrahymena were obtained and conjugated to fluorescein isothiocyanate. The fluorescein-antibody conjugates were used to directly label Tetrahymena cells. Both macro- and micronuclei were visibly fluorescent in cells stained with anti-whole histone conjugate. However, the anti- H1 conjugate only labeled macronuclei. This in situ demonstration of the lack of positive immunofluorescent staining of micronuclei with anti-H1 conjugate provide further evidence for the absence of H1 in the genetically inactive, mitotically dividing Tetrahymena micronucleus.
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This article has been cited by other articles:

• Stargell, L A, Bowen, J, Dadd, C A, Dedon, P C, Davis, M, Cook, R G, Allis, C D, Gorovsky, M A (1993). Temporal and spatial association of histone H2A variant hv1 with transcriptionally competent chromatin during nuclear development in Tetrahymena thermophila.. Genes Dev. 7: 2641-2651 [Abstract]  
• Gorovsky, M. A., Glover, C., Johmann, C. A., Keevert, J. B., Mathis, D. J., Samuelson, M. (1978). Histones and Chromatin Structure in Tetrahymena Macro- and Micronuclei. Cold Spring Harb Symp Quant Biol 42: 493-503 [Abstract]  

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