pH-dependent binding of immunoglobulins to intestinal cells of the neonatal rat

doi:10.1083/jcb.71.2.666

This Article

	Full Text (PDF, 939K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Rodewald, R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Rodewald, R.


Social Bookmarking

	What's this?

ARTICLES

pH-dependent binding of immunoglobulins to intestinal cells of the neonatal rat

R Rodewald

Rat and rabbit IgG immunoglobulins conjugated to horseradiah peroxidase as a histochemical marker bind at 0 degrees C to the luminal surface of absorptive cells in isolated segments of jejunum from 10-12-day old rats. Binding is observed at pH 6.0, near the normal luminal pH of the duodenum and jejunum at this age, but not at pH 7.4. Furthermore, no binding occurs when cells are exposed at pH 6.0 to either free peroxidase or peroxidase conjugated to chicken or sheep IgG immunoglobulins or bovine serum albumin. The sensitivity of binding to pH suggests a means whereby immunoglobulins which are selectively absorbed by the cells can be released efficiently at the abluminal surface.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Sarav, M., Wang, Y., Hack, B. K., Chang, A., Jensen, M., Bao, L., Quigg, R. J. (2009). Renal FcRn Reclaims Albumin but Facilitates Elimination of IgG. J. Am. Soc. Nephrol. 20: 1941-1952 [Abstract] [Full Text]
Tzaban, S., Massol, R. H., Yen, E., Hamman, W., Frank, S. R., Lapierre, L. A., Hansen, S. H., Goldenring, J. R., Blumberg, R. S., Lencer, W. I. (2009). The recycling and transcytotic pathways for IgG transport by FcRn are distinct and display an inherent polarity. JCB 185: 673-684 [Abstract] [Full Text]
Elizondo-Salazar, J. A., Heinrichs, A. J. (2008). Review: Heat Treating Bovine Colostrum. Professional Animal Scientist 24: 530-538 [Abstract]
Mi, W., Wanjie, S., Lo, S.-T., Gan, Z., Pickl-Herk, B., Ober, R. J., Ward, E. S. (2008). Targeting the Neonatal Fc Receptor for Antigen Delivery Using Engineered Fc Fragments. J. Immunol. 181: 7550-7561 [Abstract] [Full Text]
Goebl, N. A., Babbey, C. M., Datta-Mannan, A., Witcher, D. R., Wroblewski, V. J., Dunn, K. W. (2008). Neonatal Fc Receptor Mediates Internalization of Fc in Transfected Human Endothelial Cells. Mol. Biol. Cell 19: 5490-5505 [Abstract] [Full Text]
Qiao, S.-W., Kobayashi, K., Johansen, F.-E., Sollid, L. M., Andersen, J. T., Milford, E., Roopenian, D. C., Lencer, W. I., Blumberg, R. S. (2008). Dependence of antibody-mediated presentation of antigen on FcRn. Proc. Natl. Acad. Sci. USA 105: 9337-9342 [Abstract] [Full Text]
Dickinson, B. L., Claypool, S. M., D'Angelo, J. A., Aiken, M. L., Venu, N., Yen, E. H., Wagner, J. S., Borawski, J. A., Pierce, A. T., Hershberg, R., Blumberg, R. S., Lencer, W. I. (2008). Ca2+-dependent Calmodulin Binding to FcRn Affects Immunoglobulin G Transport in the Transcytotic Pathway. Mol. Biol. Cell 19: 414-423 [Abstract] [Full Text]
Akilesh, S., Christianson, G. J., Roopenian, D. C., Shaw, A. S. (2007). Neonatal FcR Expression in Bone Marrow-Derived Cells Functions to Protect Serum IgG from Catabolism. J. Immunol. 179: 4580-4588 [Abstract] [Full Text]
Liu, L., Garcia, A. M., Santoro, H., Zhang, Y., McDonnell, K., Dumont, J., Bitonti, A. (2007). Amelioration of Experimental Autoimmune Myasthenia Gravis in Rats by Neonatal FcR Blockade. J. Immunol. 178: 5390-5398 [Abstract] [Full Text]
Prabhat, P., Gan, Z., Chao, J., Ram, S., Vaccaro, C., Gibbons, S., Ober, R. J., Ward, E. S. (2007). Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy. Proc. Natl. Acad. Sci. USA 104: 5889-5894 [Abstract] [Full Text]
Datta-Mannan, A., Witcher, D. R., Tang, Y., Watkins, J., Wroblewski, V. J. (2007). Monoclonal Antibody Clearance: IMPACT OF MODULATING THE INTERACTION OF IgG WITH THE NEONATAL Fc RECEPTOR. J. Biol. Chem. 282: 1709-1717 [Abstract] [Full Text]
Datta-Mannan, A., Witcher, D. R., Tang, Y., Watkins, J., Jiang, W., Wroblewski, V. J. (2007). Humanized IgG1 Variants with Differential Binding Properties to the Neonatal Fc Receptor: Relationship to Pharmacokinetics in Mice and Primates. Drug Metab. Dispos. 35: 86-94 [Abstract] [Full Text]
Vaccaro, C., Bawdon, R., Wanjie, S., Ober, R. J., Ward, E. S. (2006). Divergent activities of an engineered antibody in murine and human systems have implications for therapeutic antibodies. Proc. Natl. Acad. Sci. USA 103: 18709-18714 [Abstract] [Full Text]
Dall'Acqua, W. F., Kiener, P. A., Wu, H. (2006). Properties of Human IgG1s Engineered for Enhanced Binding to the Neonatal Fc Receptor (FcRn). J. Biol. Chem. 281: 23514-23524 [Abstract] [Full Text]
Zhu, X., Peng, J., Chen, D., Liu, X., Ye, L., Iijima, H., Kadavil, K., Lencer, W. I., Blumberg, R. S. (2005). Calnexin and ERp57 Facilitate the Assembly of the Neonatal Fc Receptor for IgG with {beta}2-Microglobulin in the Endoplasmic Reticulum. J. Immunol. 175: 967-976 [Abstract] [Full Text]
Wernick, N. L. B., Haucke, V., Simister, N. E. (2005). Recognition of the Tryptophan-based Endocytosis Signal in the Neonatal Fc Receptor by the {micro} Subunit of Adaptor Protein-2. J. Biol. Chem. 280: 7309-7316 [Abstract] [Full Text]
Kenanova, V., Olafsen, T., Crow, D. M., Sundaresan, G., Subbarayan, M., Carter, N. H., Ikle, D. N., Yazaki, P. J., Chatziioannou, A. F., Gambhir, S. S., Williams, L. E., Shively, J. E., Colcher, D., Raubitschek, A. A., Wu, A. M. (2005). Tailoring the Pharmacokinetics and Positron Emission Tomography Imaging Properties of Anti-Carcinoembryonic Antigen Single-Chain Fv-Fc Antibody Fragments. Cancer Res. 65: 622-631 [Abstract] [Full Text]
Ober, R. J., Martinez, C., Lai, X., Zhou, J., Ward, E. S. (2004). Exocytosis of IgG as mediated by the receptor, FcRn: An analysis at the single-molecule level. Proc. Natl. Acad. Sci. USA 101: 11076-11081 [Abstract] [Full Text]
Claypool, S. M., Dickinson, B. L., Wagner, J. S., Johansen, F.-E., Venu, N., Borawski, J. A., Lencer, W. I., Blumberg, R. S. (2004). Bidirectional Transepithelial IgG Transport by a Strongly Polarized Basolateral Membrane Fc{gamma}-Receptor. Mol. Biol. Cell 15: 1746-1759 [Abstract] [Full Text]
Ober, R. J., Martinez, C., Vaccaro, C., Zhou, J., Ward, E. S. (2004). Visualizing the Site and Dynamics of IgG Salvage by the MHC Class I-Related Receptor, FcRn. J. Immunol. 172: 2021-2029 [Abstract] [Full Text]
Acqua, W. F. D., Woods, R. M., Ward, E. S., Palaszynski, S. R., Patel, N. K., Brewah, Y. A., Wu, H., Kiener, P. A., Langermann, S. (2002). Increasing the Affinity of a Human IgG1 for the Neonatal Fc Receptor: Biological Consequences. J. Immunol. 169: 5171-5180 [Abstract] [Full Text]
Spiekermann, G. M., Finn, P. W., Ward, E. S., Dumont, J., Dickinson, B. L., Blumberg, R. S., Lencer, W. I. (2002). Receptor-mediated Immunoglobulin G Transport Across Mucosal Barriers in Adult Life: Functional Expression of FcRn in the Mammalian Lung. JEM 196: 303-310 [Abstract] [Full Text]
Claypool, S. M., Dickinson, B. L., Yoshida, M., Lencer, W. I., Blumberg, R. S. (2002). Functional Reconstitution of Human FcRn in Madin-Darby Canine Kidney Cells Requires Co-expressed Human beta 2-Microglobulin. J. Biol. Chem. 277: 28038-28050 [Abstract] [Full Text]
Firan, M., Bawdon, R., Radu, C., Ober, R. J., Eaken, D., Antohe, F., Ghetie, V., Ward, E. S. (2001). The MHC class I-related receptor, FcRn, plays an essential role in the maternofetal transfer of {gamma}-globulin in humans. Int Immunol 13: 993-1002 [Abstract] [Full Text]
Zhu, X., Meng, G., Dickinson, B. L., Li, X., Mizoguchi, E., Miao, L., Wang, Y., Robert, C., Wu, B., Smith, P. D., Lencer, W. I., Blumberg, R. S. (2001). MHC Class I-Related Neonatal Fc Receptor for IgG Is Functionally Expressed in Monocytes, Intestinal Macrophages, and Dendritic Cells. J. Immunol. 166: 3266-3276 [Abstract] [Full Text]
McCarthy, K., Yoong, Y, Simister, N. (2000). Bidirectional transcytosis of IgG by the rat neonatal Fc receptor expressed in a rat kidney cell line: a system to study protein transport across epithelia. J. Cell Sci. 113: 1277-1285 [Abstract]
Stefaner, I., Praetor, A., Hunziker, W. (1999). Nonvectorial Surface Transport, Endocytosis via a Di-leucine-based Motif, and Bidirectional Transcytosis of Chimera Encoding the Cytosolic Tail of Rat FcRn Expressed in Madin-Darby Canine Kidney Cells. J. Biol. Chem. 274: 8998-9005 [Abstract] [Full Text]
Praetor, A, Ellinger, I, Hunziker, W (1999). Intracellular traffic of the MHC class I-like IgG Fc receptor, FcRn, expressed in epithelial MDCK cells. J. Cell Sci. 112: 2291-2299 [Abstract]
Berryman, M, Rodewald, R (1995). Beta 2-microglobulin co-distributes with the heavy chain of the intestinal IgG-Fc receptor throughout the transepithelial transport pathway of the neonatal rat. J. Cell Sci. 108: 2347-2360 [Abstract]
Simister, N.E., MosTov, K.E. (1989). Cloning and Expression of the Neonatal Rat Intestinal Fc Receptor, a Major Histocompatibility Complex Class I Antigen Homolog. Cold Spring Harb Symp Quant Biol 54: 571-580 [Abstract]
Wu, Z., Simister, N. E. (2001). Tryptophan- and Dileucine-based Endocytosis Signals in the Neonatal Fc Receptor. J. Biol. Chem. 276: 5240-5247 [Abstract] [Full Text]