The Journal of Cell Biology, Vol 72, 57-66, Copyright © 1977 by The Rockefeller University Press
RNA transport in isolated myeloma nuclei. Transport from membrane- denuded nuclei
SE Stuart, GA Clawson, FM Rottman and RJ Patterson
Nuclei prepared from MOPC-21 cells were treated with the nonionic
detergents Triton X-100 or Nonidet P-40. Chemical analysis revealed that
nearly 90% of the nuclear phospholipid was removed by detergent treatment.
The membrane-denuded nuclei remained intact with preservation of nuclear
pore complexes as demonstrated by electron microscopy. Ribonucleic acid
transport from detergent-treated nuclei proceeded at the same rate and to
the same extent as in control nuclei. Normal nuclear restriction of nucleic
acids was unaltered by removal of the nuclear membranes. The effect of
temperature on transport of RNA from freshly isolated myeloma nuclei with
intact nuclear envelopes was studied. No temperature transition was
associated with the transport process. These data indicate that the
transport of macromolecules from isolated myeloma nuclei is independent of
the nuclear membrane.
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