The Journal of Cell Biology, Vol 74, 524-530, Copyright © 1977 by The Rockefeller University Press

ARTICLES

Evidence for the glia-neuron protein transfer hypothesis from intracellular perfusion studies of squid giant axons

H Gainer, I Tasaki and RJ Lasek

Incubation of intracellulary perfused squid giant axons in [3H]leucine demonstrated that newly synthesized proteins appeared in the perfusate after a 45-min lag period. The transfer of labeled proteins was shown to occur steadily over 8 h of incubation, in the presence of an intact axonal plasma membrane as evidenced by the ability of the perfused axon to conduct propagated action potentials over this time-period. Intracellularly perfused RNase did not affect this transfer, whereas extracellularly applied puromycin, which blocked de novo protein synthesis in the glial sheath, prevented the appearance of labeled proteins in the perfusate. The uptake of exogenous 14C-labeled bovine serum albumin (BSA) into the axon had entirely different kinetics than the endogenous glial labeled protein transfer process. The data provide support for the glia-neuron protein transfer hypothesis.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Giuditta, A., Tai Chun, J., Eyman, M., Cefaliello, C., Bruno, A. P., Crispino, M. (2008). Local Gene Expression in Axons and Nerve Endings: The Glia-Neuron Unit. Physiol. Rev. 88: 515-555 [Abstract] [Full Text]  
• Bittner, G. D., Schallert, T., Peduzzi, J. D. (2000). Degeneration, Trophic Interactions, and Repair of Severed Axons: A Reconsideration of Some Common Assumptions. Neuroscientist 6: 88-109 [Abstract]  
• Campbell, R. M., Peterson, A. C. (1993). Expression of a lacZ transgene reveals floor plate cell morphology and macromolecular transfer to commissural axons. Development 119: 1217-1228 [Abstract]  
• Waxman, S., Ritchie, J. (1985). Organization of ion channels in the myelinated nerve fiber. Science 228: 1502-1507 [Abstract]  
• Muller, K., Scott, S. (1979). Correct axonal regeneration after target cell removal in the central nervous system of the leech. Science 206: 87-89 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents