Histogenesis of mouse cerebellum in microwell cultures. Cell reaggregation and migration, fiber and synapse formation

doi:10.1083/jcb.75.3.915

This Article

	Full Text (PDF, 10103K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Trenkner, E.
	Articles by Sidman, R. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Trenkner, E.
	Articles by Sidman, R. L.


Social Bookmarking

	What's this?

ARTICLES

Histogenesis of mouse cerebellum in microwell cultures. Cell reaggregation and migration, fiber and synapse formation

E Trenkner and RL Sidman

A microwell culture system was developed for analysis of cell movements and interactions during nervous system histogenesis. Cells from trypsinized 7-day-old mouse cerebellum reaggregated within hours into clusters which later developed interconnections consisting of either sheets of migrating cells and cell processes or cables of fiber bundles with cells migrating along their surfaces. Granule cells in several stages of differentiation, basket and/or stellate neurons, some larger neurons, and two types of neuroglial cells were identified in reproducible, nonrandom patterns by scanning and transmission electron microscopy. Axonal and dendritic processes, both with growth cones, and numerous synapses were generated in vitro.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Monaco, E. A. III, Vallano, M. L. (2005). Roscovitine Triggers Excitotoxicity in Cultured Granule Neurons by Enhancing Glutamate Release. Mol. Pharmacol. 68: 1331-1342 [Abstract] [Full Text]
Schnepp, A., Lindgren, P. K., Hulsmann, H., Kroger, S., Paulsson, M., Hartmann, U. (2005). Mouse Testican-2: EXPRESSION, GLYCOSYLATION, AND EFFECTS ON NEURITE OUTGROWTH. J. Biol. Chem. 280: 11274-11280 [Abstract] [Full Text]
Yacubova, E., Komuro, H. (2002). Intrinsic Program for Migration of Cerebellar Granule Cells In Vitro. J. Neurosci. 22: 5966-5981 [Abstract] [Full Text]
Holmes, T. C., de Lacalle, S., Su, X., Liu, G., Rich, A., Zhang, S. (2000). Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds. Proc. Natl. Acad. Sci. USA 97: 6728-6733 [Abstract] [Full Text]
Kolkova, K., Novitskaya, V., Pedersen, N., Berezin, V., Bock, E. (2000). Neural Cell Adhesion Molecule-Stimulated Neurite Outgrowth Depends on Activation of Protein Kinase C and the Ras-Mitogen-Activated Protein Kinase Pathway. J. Neurosci. 20: 2238-2246 [Abstract] [Full Text]
El Idrissi, A., Trenkner, E. (1999). Growth Factors and Taurine Protect against Excitotoxicity by Stabilizing Calcium Homeostasis and Energy Metabolism. J. Neurosci. 19: 9459-9468 [Abstract] [Full Text]
Jensen, P., Surmeier, D. J., Goldowitz, D. (1999). Rescue of Cerebellar Granule Cells from Death in weaver NR1 Double Mutants. J. Neurosci. 19: 7991-7998 [Abstract] [Full Text]
Komuro, H., Rakic, P. (1998). Distinct Modes of Neuronal Migration in Different Domains of Developing Cerebellar Cortex. J. Neurosci. 18: 1478-1490 [Abstract] [Full Text]
van Echten-Deckert, G., Giannis, A., Schwarz, A., Futerman, A. H., Sandhoff, K. (1998). 1-Methylthiodihydroceramide, a Novel Analog of Dihydroceramide, Stimulates Sphinganine Degradation Resulting in Decreased de Novo Sphingolipid Biosynthesis. J. Biol. Chem. 273: 1184-1191 [Abstract] [Full Text]
van Echten-Deckert, G., Zschoche, A., Bar, T., Schmidt, R. R., Raths, A., Heinemann, T., Sandhoff, K. (1997). cis-4-Methylsphingosine Decreases Sphingolipid Biosynthesis by Specifically Interfering with Serine Palmitoyltransferase Activity in Primary Cultured Neurons. J. Biol. Chem. 272: 15825-15833 [Abstract] [Full Text]
Kiselyov, V. V., Berezin, V., Maar, T. E., Soroka, V., Edvardsen, K., Schousboe, A., Bock, E. (1997). The First Immunoglobulin-like Neural Cell Adhesion Molecule (NCAM) Domain Is Involved in Double-reciprocal Interaction with the Second Immunoglobulin-like NCAM Domain and in Heparin Binding. J. Biol. Chem. 272: 10125-10134 [Abstract] [Full Text]
Schwarzmann, G�n., Hofmann, P., P�tz, U., Albrecht, B. (1995). Demonstration of Direct Glycosylation of Nondegradable Glucosylceramide Analogs in Cultured Cells. J. Biol. Chem. 270: 21271-21276 [Abstract] [Full Text]
Liem, R. K. H., Keith, C. H., Leterrier, J. F., Trenkner, E., Shelanski, M. L. (1982). Chemistry and Biology of Neuronal and Glial Intermediate Filaments. Cold Spring Harb Symp Quant Biol 46: 341-350 [Abstract]