The Journal of Cell Biology, Vol 76, 223-228, Copyright © 1978 by The Rockefeller University Press

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Alterations in number of protofilaments in microtubules assembled in vitro

GB Pierson, PR Burton and RH Himes

Tubulin from bovine brain was polymerized in vitro using a variety of assembly conditions. Many of the formed microtubules were shown to contain 14 wall protofilaments. The number of microtubules containing 14 protofilaments increased with consecutive repetitions of cold- dissociation followed by reassembly in vitro.
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