Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution

doi:10.1083/jcb.79.2.546

This Article

	Full Text (PDF, 4931K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Gerace, L.
	Articles by Blobel, G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Gerace, L.
	Articles by Blobel, G.


Social Bookmarking

	What's this?

ARTICLES

Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution

L Gerace, A Blum and G Blobel

This laboratory has previously isolated a fraction from rat liver nuclei consisting of nuclear pore complexes associated with the proteinaceous lamina which underlies the inner nuclear membrane. Using protein eluted from sodium dodecyl sulfate (SDS) gels, we have prepared antibodies in chickens to each of the three predominant pore complex- lamina bands. Ouchterlony double diffusion analysis shows that each of these individual bands cross-reacts strongly with all three antisera. In immunofluorescence localization performed on tissue culture cells with these antibodies, we obtain a pattern of intense staining at the periphery of the interphase nucleus, with little or no cytoplasmic reaction. Electron microscope immunoperoxidase staining of rat liver nuclei with these antibodies labels exclusively the nuclear periphery. Furthermore, reaction occurs in areas which contain the lamina, but not at the pore complexes. While our isolation procedure extracts the internal contents of nuclei completely, semiquantitative Ouchterlony analysis shows that it releases negligible amounts of these lamina antigens. Considered together, our results indicate that these three bands represent major components of a peripheral nuclear lamina, and are not structural elements of an internal "nuclear protein matrix." Fluorescence microscopy shows that the perinuclear interphase localization of these lamina proteins undergoes dramatic changes during mitosis. Concomitant with nuclear envelope disassembly in prophase, these antigens assume a diffuse localization throughout the cell. This distribution persists until telophase, when the antigens become progressively and completely localized at the surface of the daughter chromosome masses. We propose that the lamina is a biological polymer which can undergo reversible disassembly during mitosis.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Gambineri, A, Semple, R K, Forlani, G, Genghini, S, Grassi, I, Hyden, C S S, Pagotto, U, O'Rahilly, S, Pasquali, R (2008). Monogenic polycystic ovary syndrome due to a mutation in the lamin A/C gene is sensitive to thiazolidinediones but not to metformin. Eur J Endocrinol 159: 347-353 [Abstract] [Full Text]
Schermelleh, L., Carlton, P. M., Haase, S., Shao, L., Winoto, L., Kner, P., Burke, B., Cardoso, M. C., Agard, D. A., Gustafsson, M. G. L., Leonhardt, H., Sedat, J. W. (2008). Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy. Science 320: 1332-1336 [Abstract] [Full Text]
Dechat, T., Pfleghaar, K., Sengupta, K., Shimi, T., Shumaker, D. K., Solimando, L., Goldman, R. D. (2008). Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin. Genes Dev. 22: 832-853 [Abstract] [Full Text]
Liu, Q., Pante, N., Misteli, T., Elsagga, M., Crisp, M., Hodzic, D., Burke, B., Roux, K. J. (2007). Functional association of Sun1 with nuclear pore complexes. JCB 178: 785-798 [Abstract] [Full Text]
Broers, J. L. V., Ramaekers, F. C. S., Bonne, G., Yaou, R. B., Hutchison, C. J. (2006). Nuclear lamins: laminopathies and their role in premature ageing.. Physiol. Rev. 86: 967-1008 [Abstract] [Full Text]
Boguslavsky, R. L., Stewart, C. L., Worman, H. J. (2006). Nuclear lamin A inhibits adipocyte differentiation: implications for Dunnigan-type familial partial lipodystrophy. Hum Mol Genet 15: 653-663 [Abstract] [Full Text]
Crisp, M., Liu, Q., Roux, K., Rattner, J.B., Shanahan, C., Burke, B., Stahl, P. D., Hodzic, D. (2006). Coupling of the nucleus and cytoplasm: role of the LINC complex. JCB 172: 41-53 [Abstract] [Full Text]
Cohen, S., Pante, N. (2005). Pushing the envelope: microinjection of Minute virus of mice into Xenopus oocytes causes damage to the nuclear envelope. J. Gen. Virol. 86: 3243-3252 [Abstract] [Full Text]
Leslie, M. (2005). The isolation of the nuclear lamina. JCB 170: 865-865 [Full Text]
Senda, T., Iizuka-Kogo, A., Shimomura, A. (2005). Visualization of the Nuclear Lamina in Mouse Anterior Pituitary Cells and Immunocytochemical Detection of Lamin A/C by Quick-freeze Freeze-substitution Electron Microscopy. J. Histochem. Cytochem. 53: 497-507 [Abstract] [Full Text]
Sanfins, A., Plancha, C. E., Overstrom, E. W., Albertini, D. F. (2004). Meiotic spindle morphogenesis in in vivo and in vitro matured mouse oocytes: insights into the relationship between nuclear and cytoplasmic quality. Hum Reprod 19: 2889-2899 [Abstract] [Full Text]
Ralle, T., Grund, C., Franke, W. W., Stick, R. (2004). Intranuclear membrane structure formations by CaaX-containing nuclear proteins. J. Cell Sci. 117: 6095-6104 [Abstract] [Full Text]
Carrasco, S., Merida, I. (2004). Diacylglycerol-dependent Binding Recruits PKC{theta} and RasGRP1 C1 Domains to Specific Subcellular Localizations in Living T Lymphocytes. Mol. Biol. Cell 15: 2932-2942 [Abstract] [Full Text]
Dreuillet, C., Tillit, J., Kress, M., Ernoult-Lange, M. (2002). In vivo and in vitro interaction between human transcription factor MOK2 and nuclear lamin A/C. Nucleic Acids Res 30: 4634-4642 [Abstract] [Full Text]
Schmitt, I., Gerace, L. (2001). In Vitro Analysis of Nuclear Transport Mediated by the C-terminal Shuttle Domain of Tap. J. Biol. Chem. 276: 42355-42363 [Abstract] [Full Text]
Lyman, S. K., Gerace, L. (2001). Nuclear pore complexes: dynamics in unexpected places. JCB 154: 17-20 [Abstract] [Full Text]
Lopez-Soler, R. I., Moir, R. D., Spann, T. P., Stick, R., Goldman, R. D. (2001). A role for nuclear lamins in nuclear envelope assembly. JCB 154: 61-70 [Abstract] [Full Text]
Kennedy, B. K., Barbie, D. A., Classon, M., Dyson, N., Harlow, E. (2000). Nuclear organization of DNA replication in primary mammalian cells. Genes Dev. 14: 2855-2868 [Abstract] [Full Text]
Lee, K. K., Gruenbaum, Y., Spann, P., Liu, J., Wilson, K. L. (2000). C. elegans Nuclear Envelope Proteins Emerin, MAN1, Lamin, and Nucleoporins Reveal Unique Timing of Nuclear Envelope Breakdown during Mitosis. Mol. Biol. Cell 11: 3089-3099 [Abstract] [Full Text]
Moir, R. D., Spann, T. P., Herrmann, H., Goldman, R. D. (2000). Disruption of Nuclear Lamin Organization Blocks the Elongation Phase of DNA Replication. JCB 149: 1179-1192 [Abstract] [Full Text]
Croft, J. A., Bridger, J. M., Boyle, S., Perry, P., Teague, P., Bickmore, W. A. (1999). Differences in the Localization and Morphology of Chromosomes in the Human Nucleus. JCB 145: 1119-1131 [Abstract] [Full Text]
Kehlenbach, R. H., Dickmanns, A., Kehlenbach, A., Guan, T., Gerace, L. (1999). A Role for RanBP1 in the Release of CRM1 from the Nuclear Pore Complex in a Terminal Step of Nuclear Export. JCB 145: 645-657 [Abstract] [Full Text]
Strambio-de-Castillia, C., Blobel, G., Rout, M. P. (1999). Proteins Connecting the Nuclear Pore Complex with the Nuclear Interior. JCB 144: 839-855 [Abstract] [Full Text]
Jagatheesan, G, Thanumalayan, S, Muralikrishna, B, Rangaraj, N, Karande, A., Parnaik, V. (1999). Colocalization of intranuclear lamin foci with RNA splicing factors. J. Cell Sci. 112: 4651-4661 [Abstract]
Lenz-Bohme, B., Wismar, J., Fuchs, S., Reifegerste, R., Buchner, E., Betz, H., Schmitt, B. (1997). Insertional Mutation of the Drosophila Nuclear Lamin Dm0 Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae. JCB 137: 1001-1016 [Abstract] [Full Text]
Nikolakaki, E., Meier, J., Simos, G., Georgatos, S. D., Giannakouros, T. (1997). Mitotic Phosphorylation of the Lamin B Receptor by a Serine/Arginine Kinase and p34cdc2. J. Biol. Chem. 272: 6208-6213 [Abstract] [Full Text]
Pugh, G., Coates, P., Lane, E., Raymond, Y, Quinlan, R. (1997). Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells. J. Cell Sci. 110: 2483-2493 [Abstract]
Zhang, C, Jenkins, H, Goldberg, M., Allen, T., Hutchison, C. (1996). Nuclear lamina and nuclear matrix organization in sperm pronuclei assembled in Xenopus egg extract. J. Cell Sci. 109: 2275-2286 [Abstract]
Hozak, P, Sasseville, A., Raymond, Y, Cook, P. (1995). Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells. J. Cell Sci. 108: 635-644 [Abstract]
Matsuoka, Y, Takechi, S, Nakayama, T, Yoneda, Y (1994). Exogenous histone H1 injection into mitotic cells disrupts synchronous progression of mitotic events by delaying chromosome decondensation. J. Cell Sci. 107: 693-701 [Abstract]
Kaneda, Y, Kinoshita, K, Sato, M, Tanaka, K, Kaneda, Y (1993). The analysis of 40 kDa nuclear protein, p40, in interphase cells and mitotic cells. J. Cell Sci. 106: 741-748 [Abstract]
Warren, S. L., Landolfi, A. S., Curtis, C., Morrow, J. S. (1992). Cytostellin: a novel, highly conserved protein that undergoes continuous redistribution during the cell cycle. J. Cell Sci. 103: 381-388 [Abstract]
Lin, H., Song, K., Hutcheson, G.A., Goutte, C.E., Wolfner, M.F. (1991). A Maternally Encoded Nuclear Envelope Protein Required for Embryonic Mitosis in Drosophila. Cold Spring Harb Symp Quant Biol 56: 719-727 [Abstract]
LASSOUED, K., GUILLY, M.-N., DANON, F., ANDRE, C., DHUMEAUX, D., CLAUVEL, J.-P., BROUET, J.-C., SELIGMANN, M., COURVALIN, J.-C. (1988). Antinuclear Autoantibodies Specific for Lamins: Characterization and Clinical Significance. ANN INTERN MED 108: 829-833 [Abstract]
Gerace, L., Blobel, G. (1982). Nuclear Lamina and the Structural Organization of the Nuclear Envelope. Cold Spring Harb Symp Quant Biol 46: 967-978 [Abstract]
Anglana, M., Debatisse, M. (2001). Dual Control of Replication Timing. STOCHASTIC ONSET BUT PROGRAMMED COMPLETION OF MAMMALIAN CHROMOSOME DUPLICATION. J. Biol. Chem. 276: 36639-36646 [Abstract] [Full Text]