Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria

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Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria

A Quaroni, J Wands, RL Trelstad and KJ Isselbacher

Rat small intestinal epithelial cell lines have been established in vitro and subcultured serially for periods up to 6 mo. These cells have an epithelioid morphology, grow as monolayers of closely opposed polygonal cells, and during the logarithmic phase of growth have a population doubling time of 19--22 h. Ultrastructural studies revealed the presence of microvilli, tight junctions, an extensive Golgi complex, and the presence of extracellular amorphous material similar in appearance to isolated basement membrane. These cells exhibit a number of features characteristic of normal cells in culture; namely, a normal rat diploid karyotype, strong density inhibition of growth, lack of growth in soft agar, and a low plating efficiency when seeded at low density. They did not produce tumors when injected in syngeneic animals. Immunochemical studies were performed to determine their origin using antisera prepared against rat small intestinal crypt cell plasma membrane, brush border membrane of villus cells and isolated sucrase-isomaltase complex. Antigenic determinants specific for small intestinal epithelial (crypt and villus) cells were demonstrated on the surface of the epithelioid cells, but they lacked immunological determinants specific for differentiated villus cells. An antiserum specifically staining extracellular material surrounding the cells cultured in vitro demonstrated cross-reactivity to basement membrane in rat intestinal frozen sections. It is concluded that the cultured epithelioid cells have features of undifferentiated small intestinal crypt cells.
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Ray, R. M., McCormack, S. A., Johnson, L. R. (2001). Polyamine depletion arrests growth of IEC-6 and Caco-2 cells by different mechanisms. Am. J. Physiol. Gastrointest. Liver Physiol. 281: G37-G43 [Abstract] [Full Text]
Li, L., Rao, J. N., Bass, B. L., Wang, J.-Y. (2001). NF-{kappa}B activation and susceptibility to apoptosis after polyamine depletion in intestinal epithelial cells. Am. J. Physiol. Gastrointest. Liver Physiol. 280: G992-G1004 [Abstract] [Full Text]
Chiu, T., Rozengurt, E. (2001). PKD in intestinal epithelial cells: rapid activation by phorbol esters, LPA, and angiotensin through PKC. Am. J. Physiol. Cell Physiol. 280: C929-C942 [Abstract] [Full Text]
Rao, J. N., Li, L., Golovina, V. A., Platoshyn, O., Strauch, E. D., Yuan, J. X.-J., Wang, J.-Y. (2001). Ca2+-RhoA signaling pathway required for polyamine-dependent intestinal epithelial cell migration. Am. J. Physiol. Cell Physiol. 280: C993-C1007 [Abstract] [Full Text]
Langer, R. C., Schaefer, D. A., Riggs, M. W. (2001). Characterization of an Intestinal Epithelial Cell Receptor Recognized by the Cryptosporidium parvum Sporozoite Ligand CSL. Infect. Immun. 69: 1661-1670 [Abstract] [Full Text]
Nikawa, T., Ikemoto, M., Tokuoka, K., Teshima, S., Alpers, D. H., Masui, Y., Kishi, K., Rokutan, K. (2001). Interleukin-1{beta} enhances retinoic acid-mediated expression of bone-type alkaline phosphatase in rat IEC-6 cells. Am. J. Physiol. Gastrointest. Liver Physiol. 280: G510-G517 [Abstract] [Full Text]
Naishiro, Y., Yamada, T., Takaoka, A. S., Hayashi, R., Hasegawa, F., Imai, K., Hirohashi, S. (2001). Restoration of Epithelial Cell Polarity in a Colorectal Cancer Cell Line by Suppression of {beta}-catenin/T-Cell Factor 4-mediated Gene Transactivation. Cancer Res. 61: 2751-2758 [Abstract] [Full Text]
Yuan, Q., Ray, R. M., Viar, M. J., Johnson, L. R. (2001). Polyamine regulation of ornithine decarboxylase and its antizyme in intestinal epithelial cells. Am. J. Physiol. Gastrointest. Liver Physiol. 280: G130-G138 [Abstract] [Full Text]
Varedi, M., Chinery, R., Greeley, G. H. Jr., Herndon, D. N., Englander, E. W. (2001). Thermal injury effects on intestinal crypt cell proliferation and death are cell position dependent. Am. J. Physiol. Gastrointest. Liver Physiol. 280: G157-G163 [Abstract] [Full Text]
Rao, J. N., Li, L., Bass, B. L., Wang, J.-Y. (2000). Expression of the TGF-beta receptor gene and sensitivity to growth inhibition following polyamine depletion. Am. J. Physiol. Cell Physiol. 279: C1034-C1044 [Abstract] [Full Text]
Hoffman, R. A. (2000). Intraepithelial lymphocytes coinduce nitric oxide synthase in intestinal epithelial cells. Am. J. Physiol. Gastrointest. Liver Physiol. 278: G886-G894 [Abstract] [Full Text]
Ray, R. M., Viar, M. J., Yuan, Q., Johnson, L. R. (2000). Polyamine depletion delays apoptosis of rat intestinal epithelial cells. Am. J. Physiol. Cell Physiol. 278: C480-C489 [Abstract] [Full Text]
Lynch, J., Suh, E.-R., Silberg, D. G., Rulyak, S., Blanchard, N., Traber, P. G. (2000). The Caudal-related Homeodomain Protein Cdx1 Inhibits Proliferation of Intestinal Epithelial Cells by Down-regulation of D-type Cyclins. J. Biol. Chem. 275: 4499-4506 [Abstract] [Full Text]
Wang, J.-Y., Wang, J., Golovina, V. A., Li, L., Platoshyn, O., Yuan, J. X.-J. (2000). Role of K+ channel expression in polyamine-dependent intestinal epithelial cell migration. Am. J. Physiol. Cell Physiol. 278: C303-C314 [Abstract] [Full Text]
Levy, E., Beaulieu, J.-F., Delvin, E., Seidman, E., Yotov, W., Basque, J.-R., Ménard, D. (2000). Human crypt intestinal epithelial cells are capable of lipid production, apolipoprotein synthesis, and lipoprotein assembly. J. Lipid Res. 41: 12-22 [Abstract] [Full Text]
Yuan, Q., Viar, M. J., Ray, R. M., Johnson, L. R. (2000). Putrescine does not support the migration and growth of IEC-6 cells. Am. J. Physiol. Gastrointest. Liver Physiol. 278: G49-G56 [Abstract] [Full Text]
Rao, J. N., Li, J., Li, L., Bass, B. L., Wang, J.-Y. (1999). Differentiated intestinal epithelial cells exhibit increased migration through polyamines and myosin II. Am. J. Physiol. Gastrointest. Liver Physiol. 277: G1149-G1158 [Abstract] [Full Text]
Quaroni, A., Tian, J. Q., Goke, M., Podolsky, D. K. (1999). Glucocorticoids have pleiotropic effects on small intestinal crypt cells. Am. J. Physiol. Gastrointest. Liver Physiol. 277: G1027-G1040 [Abstract] [Full Text]
Rivard, N., Boucher, M.-J., Asselin, C., L'Allemain, G. (1999). MAP kinase cascade is required for p27 downregulation and S phase entry in fibroblasts and epithelial cells. Am. J. Physiol. Cell Physiol. 277: C652-C664 [Abstract] [Full Text]