Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas

doi:10.1083/jcb.86.2.590

This Article

	Full Text (PDF, 1132K)
	Alert me when this article is cited

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Taylor, D. L.
	Articles by Heiple, J. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Taylor, D. L.
	Articles by Heiple, J. M.


Social Bookmarking

	What's this?

ARTICLES

Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas

DL Taylor, YL Wang and JM Heiple

The technique of molecular cytochemistry has been used to follow the distribution of fluorescently labeled actin in living Chaos carolinensis and Amoeba proteus during ameboid movement and various cellular processes. The distribution of 5-iodoacetamidofluorescein- labeled actin was compared with that of Lissamine rhodamine B sulfonyl chloride-labeled ovalbumin microinjected into the same cell and recorded with an image intensification microscope system. Actively motile cells demonstrated a rather uniform distribution of actin throughout most of the cytoplasm, except in the tail ectoplasm and in plasma gel sheets, where distinct actin structures were observed. In addition, actin-containing structures were induced in the cortex during wound healing, concanavalin A capping, pinocytosis, and contractions elicited by phalloidin injections. The formation of distinct fluorescent actin structures has been correlated with contractile activities.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Furukawa, R., Maselli, A., Thomson, S. A. M., Lim, R. W. L., Stokes, J. V., Fechheimer, M. (2003). Calcium regulation of actin crosslinking is important for function of the actin cytoskeleton in Dictyostelium. J. Cell Sci. 116: 187-196 [Abstract] [Full Text]
Burton, K., Park, J. H., Taylor, D. L. (1999). Keratocytes Generate Traction Forces in Two Phases. Mol. Biol. Cell 10: 3745-3769 [Abstract] [Full Text]
Terasaki, M., Miyake, K., McNeil, P. L. (1997). Large Plasma Membrane Disruptions Are Rapidly Resealed by Ca2+-dependent Vesicle-Vesicle Fusion Events. JCB 139: 63-74 [Abstract] [Full Text]
Taylor, D. L., Heiple, J., Wang, Y.-L., Luna, E. J., Tanasugarn, L., Brier, J., Swanson, J., Fechheimer, M., Amato, P., Rockwell, M., Daley, G. (1982). Cellular and Molecular Aspects of Amoeboid Movement. Cold Spring Harb Symp Quant Biol 46: 101-111 [Abstract]