Slow components of axonal transport: two cytoskeletal networks

doi:10.1083/jcb.86.2.616

This Article

	Full Text (PDF, 976K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Black, M. M.
	Articles by Lasek, R. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Black, M. M.
	Articles by Lasek, R. J.


Social Bookmarking

	What's this?

ARTICLES

Slow components of axonal transport: two cytoskeletal networks

MM Black and RJ Lasek

We have identified two slowly moving groups of axonally transported proteins in guinea pig retinal ganglion cell axons (4). The slowest group of proteins, designated slow component a (SCa), has a transport rate of 0.25 mm/d and consists of tubulin and neurofilament protein. The other slowly transported group of proteins, designated slow components b (SCb), has a transport rate of 2-3 mm/d and consists of many polypeptides, one of which is actin (4). Our analyses of the transport kinetics of the individual polypeptides of SCa and SCb indicate that (a) the polypeptides of SCa are transported coherently in the optic axons, (b) the polypeptides of SCb are also transported coherently but completely separately from the SCa polypeptides, and (c) the polypeptides of SCa differ completely from those comprising SCb. We relate these results to our general hypothesis that slow axonal transport represents the movements of structural complexes of proteins. Furthermore, it is proposed that SCa corresponds to the microtubule- neurofilament network, and that SCb represents the transport of the microfilament network together with the proteins complexed with microfilaments.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Roy, S., Winton, M. J., Black, M. M., Trojanowski, J. Q., Lee, V. M.-Y. (2008). Cytoskeletal Requirements in Axonal Transport of Slow Component-b. J. Neurosci. 28: 5248-5256 [Abstract] [Full Text]
Giuditta, A., Tai Chun, J., Eyman, M., Cefaliello, C., Bruno, A. P., Crispino, M. (2008). Local Gene Expression in Axons and Nerve Endings: The Glia-Neuron Unit. Physiol. Rev. 88: 515-555 [Abstract] [Full Text]
Roy, S., Winton, M. J., Black, M. M., Trojanowski, J. Q., Lee, V. M.-Y. (2007). Rapid and Intermittent Cotransport of Slow Component-b Proteins. J. Neurosci. 27: 3131-3138 [Abstract] [Full Text]
He, Y., Francis, F., Myers, K. A., Yu, W., Black, M. M., Baas, P. W. (2005). Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments. JCB 168: 697-703 [Abstract] [Full Text]
Verma, P., Chierzi, S., Codd, A. M., Campbell, D. S., Meyer, R. L., Holt, C. E., Fawcett, J. W. (2005). Axonal Protein Synthesis and Degradation Are Necessary for Efficient Growth Cone Regeneration. J. Neurosci. 25: 331-342 [Abstract] [Full Text]
Rao, M. V., Campbell, J., Yuan, A., Kumar, A., Gotow, T., Uchiyama, Y., Nixon, R. A. (2003). The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate. JCB 163: 1021-1031 [Abstract] [Full Text]
Rao, M. V., Garcia, M. L., Miyazaki, Y., Gotow, T., Yuan, A., Mattina, S., Ward, C. M., Calcutt, N. A., Uchiyama, Y., Nixon, R. A., Cleveland, D. W. (2002). Gene replacement in mice reveals that the heavily phosphorylated tail of neurofilament heavy subunit does not affect axonal caliber or the transit of cargoes in slow axonal transport. JCB 158: 681-693 [Abstract] [Full Text]
Zheng, J.-Q., Kelly, T. K., Chang, B., Ryazantsev, S., Rajasekaran, A. K., Martin, K. C., Twiss, J. L. (2001). A Functional Role for Intra-Axonal Protein Synthesis during Axonal Regeneration from Adult Sensory Neurons. J. Neurosci. 21: 9291-9303 [Abstract] [Full Text]
Koenig, E., Martin, R., Titmus, M., Sotelo-Silveira, J. R. (2000). Cryptic Peripheral Ribosomal Domains Distributed Intermittently along Mammalian Myelinated Axons. J. Neurosci. 20: 8390-8400 [Abstract] [Full Text]
Roy, S., Coffee, P., Smith, G., Liem, R. K. H., Brady, S. T., Black, M. M. (2000). Neurofilaments Are Transported Rapidly But Intermittently in Axons: Implications for Slow Axonal Transport. J. Neurosci. 20: 6849-6861 [Abstract] [Full Text]
Ma, D., Himes, B. T., Shea, T. B., Fischer, I. (2000). Axonal Transport of Microtubule-Associated Protein 1B (MAP1B) in the Sciatic Nerve of Adult Rat: Distinct Transport Rates of Different Isoforms. J. Neurosci. 20: 2112-2120 [Abstract] [Full Text]
Galbraith, J. A., Reese, T. S., Schlief, M. L., Gallant, P. E. (1999). Slow transport of unpolymerized tubulin and polymerized neurofilament in the squid giant axon. Proc. Natl. Acad. Sci. USA 96: 11589-11594 [Abstract] [Full Text]
Chang, S., Rodionov, V. I., Borisy, G. G., Popov, S. V. (1998). Transport and Turnover of Microtubules in Frog Neurons Depend on the Pattern of Axonal Growth. J. Neurosci. 18: 821-829 [Abstract] [Full Text]
Dillman III, J. F., Dabney, L. P., Karki, S., Paschal, B. M., Holzbaur, E. L.�F., Pfister, K. K. (1996). Functional Analysis of Dynactin and Cytoplasmic Dynein in Slow Axonal Transport. J. Neurosci. 16: 6742-6752 [Abstract] [Full Text]
Ahmad, F., Baas, P. (1995). Microtubules released from the neuronal centrosome are transported into the axon. J. Cell Sci. 108: 2761-2769 [Abstract]
Jiang, Y. Q., Oblinger, M. M. (1992). Differential regulation of beta III and other tubulin genes during peripheral and central neuron development. J. Cell Sci. 103: 643-651 [Abstract]
DAHLIN, L. B., LUNDBORG, G. (1990). The Neurone and Its Response to Peripheral Nerve Compression. J Hand Surg Eur Vol 15: 5-10 [Abstract]
Weisenberg, R., Flynn, J, Gao, B., Awodi, S, Skee, F, Goodman, SR, Riederer, B. (1987). Microtubule gelation-contraction: essential components and relation to slow axonal transport. Science 238: 1119-1122 [Abstract]
Keith, C. (1987). Slow transport of tubulin in the neurites of differentiated PC12 cells. Science 235: 337-339 [Abstract]
Lasek, R.J., Oblinger, M.M., Drake, P.F. (1983). Molecular Biology of Neuronal Geometry: Expression of Neurofilament Genes Influences Axonal Diameter. Cold Spring Harb Symp Quant Biol 48: 731-744 [Abstract]
Lasek, R. J., Brady, S. T. (1982). The Axon: A Prototype for Studying Expressional Cytoplasm. Cold Spring Harb Symp Quant Biol 46: 113-124 [Abstract]
Runge, M. S., Williams, R. C. Jr. (1982). Formation of an ATP-dependent Microtubule-Neurofilament Complex In Vitro. Cold Spring Harb Symp Quant Biol 46: 483-493 [Abstract]
Tytell, M, Black, M., Garner, J., Lasek, R. (1981). Axonal transport: each major rate component reflects the movement of distinct macromolecular complexes. Science 214: 179-181 [Abstract]