The Journal of Cell Biology, Vol 87, 802-808, Copyright © 1980 by The Rockefeller University Press

ARTICLES

Widespread distribution of a 210,000 mol wt microtubule-associated protein in cells and tissues of primates

JC Bulinski and GG Borisy

Antisera prepared against a 210,000 mol wt microtubule-associated protein (210k MAP) isolated from the human cell line, HeLa, were used to survey a variety of cells and tissues for the presence of immunologically related proteins. The antisera were employed to test extracts of the cells and tissues, using a sensitive indirect immunofluorescence technique applied to polyacrylamide gels. Cross- reactive material of 210,000 mol wt was found in 10 kinds of cells and tissues derived from humans and four lines of cells from monkeys. Indirect immunofluorescent staining was also carried out on fixed cells and showed that the cross-reactive material was localized to interphase and mitotic microtubules as assayed in nine human and seven monkey cell lines. No protein that cross-reacted with 210k MAP antisera was detected in cells and tissues derived from two rodents, an ungulate, a marsupial, or a chicken. Therefore, the 210k MAP isolated from HeLa cells is present in a wide variety of cells and tissues of humans and other primates but is antigenically distinct from MAPs present in lower organisms.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Mandelkow, E.-M., Thies, E., Trinczek, B., Biernat, J., Mandelkow, E. (2004). MARK/PAR1 kinase is a regulator of microtubule-dependent transport in axons. JCB 167: 99-110 [Abstract] [Full Text]  
• Stein, P. A., Toret, C. P., Salic, A. N., Rolls, M. M., Rapoport, T. A. (2002). A novel centrosome-associated protein with affinity for microtubules. J. Cell Sci. 115: 3389-3402 [Abstract] [Full Text]  
• Roobol, A., Sahyoun, Z. P., Carden, M. J. (1999). Selected Subunits of the Cytosolic Chaperonin Associate with Microtubules Assembled in Vitro. J. Biol. Chem. 274: 2408-2415 [Abstract] [Full Text]  
• Nguyen, H., Gruber, D, Bulinski, J. (1999). Microtubule-associated protein 4 (MAP4) regulates assembly, protomer-polymer partitioning and synthesis of tubulin in cultured cells. J. Cell Sci. 112: 1813-1824 [Abstract]  
• Elliott, G., O'Hare, P. (1998). Herpes Simplex Virus Type 1 Tegument Protein VP22 Induces the Stabilization and Hyperacetylation of Microtubules. J. Virol. 72: 6448-6455 [Abstract] [Full Text]  
• Bulinski, J., McGraw, T., Gruber, D, Nguyen, H., Sheetz, M. (1997). Overexpression of MAP4 inhibits organelle motility and trafficking in vivo. J. Cell Sci. 110: 3055-3064 [Abstract]  
• Nguyen, H., Chari, S, Gruber, D, Lue, C., Chapin, S., Bulinski, J. (1997). Overexpression of full- or partial-length MAP4 stabilizes microtubules and alters cell growth. J. Cell Sci. 110: 281-294 [Abstract]  
• Illenberger, S., Drewes, G., Trinczek, B., Biernat, J., Meyer, H. E., Olmsted, J. B., Mandelkow, E.-M., Mandelkow, E. (1996). Phosphorylation of Microtubule-associated Proteins MAP2 and MAP4 by the Protein Kinase p110[IMAGE]. J. Biol. Chem. 271: 10834-10843 [Abstract] [Full Text]  
• Goedert, M, Baur, C., Ahringer, J, Jakes, R, Hasegawa, M, Spillantini, M., Smith, M., Hill, F (1996). PTL-1, a microtubule-associated protein with tau-like repeats from the nematode Caenorhabditis elegans. J. Cell Sci. 109: 2661-2672 [Abstract]  
• Mangan, M., Olmsted, J. (1996). A muscle-specific variant of microtubule-associated protein 4 (MAP4) is required in myogenesis. Development 122: 771-781 [Abstract]  
• Bulinski, J., Bossler, A (1994). Purification and characterization of ensconsin, a novel microtubule stabilizing protein. J. Cell Sci. 107: 2839-2849 [Abstract]  
• Draberova, E, Draber, P (1993). A microtubule-interacting protein involved in coalignment of vimentin intermediate filaments with microtubules. J. Cell Sci. 106: 1263-1273 [Abstract]  
• Solomon, F. (1982). Organizing the Cytoplasm for Motility. Cold Spring Harb Symp Quant Biol 46: 17-22 [Abstract]  
• Wiche, G., Herrmann, H., Leichtfried, F., Pytela, R. (1982). Plectin: A High-molecular-weight Cytoskeletal Polypeptide Component That Copurifies with Intermediate Filaments of the Vimentin Type. Cold Spring Harb Symp Quant Biol 46: 475-482 [Abstract]  
• Brinkley, B. R. (1982). Summary: Organization of the Cytoplasm. Cold Spring Harb Symp Quant Biol 46: 1029-1040 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents