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The Journal of Cell Biology, Vol 87, 833-836, Copyright © 1980 by The Rockefeller University Press

ARTICLES

Visualization of nucleosomes in thin sections by stereo electron microscopy

AL Olins, DE Olins, H Zentgraf and WW Franke

Nucleosomes (approximately diameter) were clearly visualized in thin sections (approximately 0.1 micrometer thick) of isolated chicken erythrocytes. The cells were lysed and fixed in low ionic strength buffers that maintained the chromatin as dispersed filaments and prevented the reformation of supranucleosomal structures. Stereo electron micrographs at high magnification demonstrate the stability of nucleosome structure in the dispersed chromatin state during fixation, dehydration, and embedding.
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