The Journal of Cell Biology, Vol 89, 54-61, Copyright © 1981 by The Rockefeller University Press
Cell assembly patterns of embryonic mouse cerebellar cells on carbohydrate-derivatized polylysine culture substrata
ME Hatten
Four carbohydrate derivatives of poly-D-lysine have been synthesized and
assayed as substrates for the tissue culture of embryonic mouse cerebellar
cells. On poly-beta-(D-glucopyranosyl)-poly-D-lysine and on
poly-beta-(N-acetyl-D-glucosaminyl)-poly-D-lysine, dissociated cerebellar
cells formed a monolayer. On poly-beta-(D-galactopyranosyl)- poly-D-lysine,
cellular aggregates were formed and cables of processes were extended
between the aggregates. On poly-beta-(L-fucosyl)-poly-D- lysine, cerebellar
cells failed to attach and died within 24 h. On poly-
(N-acetyl)-poly-D-lysine, cell attachment was identical to that on poly-
D-lysine. At low concentrations of underivatized poly-D-lysine (0.5-2.0
microgram/ml) dissociated embryonic cerebellar cells formed cellular
aggregates, whereas at higher concentrations of poly-D-lysine monolayering
was extensive.
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