Taxol induces postmitotic myoblasts to assemble interdigitating microtubule-myosin arrays that exclude actin filaments

doi:10.1083/jcb.90.2.300

This Article

	Full Text (PDF, 1183K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Antin, P. B.
	Articles by Holtzer, H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Antin, P. B.
	Articles by Holtzer, H.


Social Bookmarking

	What's this?

ARTICLES

Taxol induces postmitotic myoblasts to assemble interdigitating microtubule-myosin arrays that exclude actin filaments

PB Antin, S Forry-Schaudies, TM Friedman, SJ Tapscott and H Holtzer

Taxol has the following effects on myogenic cultures: (a) it blocks cell replication of presumptive myoblasts and fibroblasts. (b) It induces the aggregation of microtubules into sheets or massive cables in presumptive myoblasts and fibroblasts, but not in postmitotic, mononucleated myoblasts. (c) It induces normally elongated postmitotic myoblasts to form stubby, star-shaped cells. (d) It reversibly blocks the fusion of the star-shaped myoblasts into multinucleated myotubes. (e) It augments the number of microtubules in postmitotic myoblasts, and these are assembled into interdigitating arrays of microtubules and myosin filaments. (f) Actin filaments are largely excluded from these interdigitating microtubule-myosin complexes. (g) The myosin filaments in the interdigitating microtubule-myosin arrays are aligned laterally, forming A-bands approximately 1.5 micrometers long.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Bartolini, F., Gundersen, G. G. (2006). Generation of noncentrosomal microtubule arrays. J. Cell Sci. 119: 4155-4163 [Abstract] [Full Text]
Swailes, N. T., Colegrave, M., Knight, P. J., Peckham, M. (2006). Non-muscle myosins 2A and 2B drive changes in cell morphology that occur as myoblasts align and fuse. J. Cell Sci. 119: 3561-3570 [Abstract] [Full Text]
McElhinny, A. S., Perry, C. N., Witt, C. C., Labeit, S., Gregorio, C. C. (2004). Muscle-specific RING finger-2 (MURF-2) is important for microtubule, intermediate filament and sarcomeric M-line maintenance in striated muscle development. J. Cell Sci. 117: 3175-3188 [Abstract] [Full Text]
McElhinny, A. S., Kakinuma, K., Sorimachi, H., Labeit, S., Gregorio, C. C. (2002). Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1. JCB 157: 125-136 [Abstract] [Full Text]
Pizon, V., Iakovenko, A., van der Ven, P. F. M., Kelly, R., Fatu, C., Furst, D. O., Karsenti, E., Gautel, M. (2002). Transient association of titin and myosin with microtubules in nascent myofibrils directed by the MURF2 RING-finger protein. J. Cell Sci. 115: 4469-4482 [Abstract] [Full Text]
Ginkel, L. M., Wordeman, L. (2000). Expression and Partial Characterization of Kinesin-related Proteins in Differentiating and Adult Skeletal Muscle. Mol. Biol. Cell 11: 4143-4158 [Abstract] [Full Text]
Spencer, J. A., Eliazer, S., Ilaria, R. L. Jr., Richardson, J. A., Olson, E. N. (2000). Regulation of Microtubule Dynamics and Myogenic Differentiation by Murf, a Striated Muscle Ring-Finger Protein. JCB 150: 771-784 [Abstract] [Full Text]
Webster, D. R., Patrick, D. L. (2000). Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset. Am. J. Physiol. Heart Circ. Physiol. 278: H1653-H1661 [Abstract] [Full Text]
Linke, W. A., Rudy, D. E., Centner, T., Gautel, M., Witt, C., Labeit, S., Gregorio, C. C. (1999). I-Band Titin in Cardiac Muscle Is a Three-Element Molecular Spring and Is Critical for Maintaining Thin Filament Structure. JCB 146: 631-644 [Abstract] [Full Text]
Kaufmann, U, Kirsch, J, Irintchev, A, Wernig, A, Starzinski-Powitz, A (1999). The M-cadherin catenin complex interacts with microtubules in skeletal muscle cells: implications for the fusion of myoblasts. J. Cell Sci. 112: 55-68 [Abstract]
Mangan, M., Olmsted, J. (1996). A muscle-specific variant of microtubule-associated protein 4 (MAP4) is required in myogenesis. Development 122: 771-781 [Abstract]
Epstein, H., Fischman, D. (1991). Molecular analysis of protein assembly in muscle development. Science 251: 1039-1044 [Abstract]
Verdoorn, C., de Lavalette, V. W. R., Dalma-Weizhausz, J., Orr, G. M., Sorgente, N., Ryan, S. J. (1986). Cellular Migration, Proliferation, and Contraction: An In Vitro Approach to a Clinical Problem Proliferative Vitreoretinopathy. Arch Ophthalmol 104: 1216-1219 [Abstract]
McElhinny, A. S., Kakinuma, K., Sorimachi, H., Labeit, S., Gregorio, C. C. (2002). Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1. JCB 157: 125-136 [Abstract] [Full Text]