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The Journal of Cell Biology, Vol 91, 410-413, Copyright © 1981 by The Rockefeller University Press


ARTICLES

Cholera toxin can catalyze ADP-ribosylation of cytoskeletal proteins

HR Kaslow, VE Groppi, ME Abood and HR Bourne

Cholera toxin catalyzes transfer of radiolabel from [32P]NAD+ to several peptides in particulate preparations of human foreskin fibroblasts. Resolution of these peptides by two-dimensional gel electrophoresis allowed identification of two peptides of Mr = 42,000 and 52,000 as peptide subunits of a regulatory component of adenylate cyclase. The radiolabeling of another group of peptides (Mr = 50,000 to 65,000) suggested that cholera toxin could catalyze ADP-ribosylation of cytoskeletal proteins. This suggestion was confirmed by showing that incubation with cholera toxin and [32P]NAD+ caused radiolabeling of purified microtubule and intermediate filament proteins.
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