Serial propagation of human endothelial cells in vitro

doi:10.1083/jcb.91.2.420

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Serial propagation of human endothelial cells in vitro

T Maciag, GA Hoover, MB Stemerman and R Weinstein

Human umbilical vein (HUV) endothelial cells were grown for 15 to 21 passages at a split ratio of 1:5 (at least 27 population doublings) on a human fibronectin (HFN) matrix in Medium 199 supplemented with fetal bovine serum (FBS) and endothelial-cell growth factor (ECGF). This system also permitted the growth of HUV endothelial cells at cell densities as low as 1.25 cells/cm2. In addition to delaying the premature senescence of HUV endothelial cells, ECGF also reduced the serum requirement for low-density HUV endothelial-cell growth; 2.5% serum and ECGF yields half-maximum growth as compared to high serum controls. Significant HUV endothelial-cell growth was also observed in medium supplemented with either ovine hypophysectomized (HYPOX) serum, plasma-derived serum (PDS), or HYPOX-PDS in the presence of ECGF, suggesting that neither the pituitary nor the platelet contributes to HUV endothelial-cell growth.
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MacNeill, B. D, Pomerantseva, I., Lowe, H. C, Oesterle, S. N, Vacanti, J. P (2002). Toward a new blood vessel. Vasc Med 7: 241-246 [Abstract]
Gerli, R., Gresele, P., Bistoni, O., Paolucci, C., Lanfrancone, L., Fiorucci, S., Muscat, C., Costantini, V. (2001). Salicylates Inhibit T Cell Adhesion on Endothelium Under Nonstatic Conditions: Induction of L-Selectin Shedding by a Tyrosine Kinase-Dependent Mechanism. J. Immunol. 166: 832-840 [Abstract] [Full Text]
Gerli, R., Pitzalis, C., Bistoni, O., Falini, B., Costantini, V., Russano, A., Lunardi, C. (2000). CD30+ T Cells in Rheumatoid Synovitis: Mechanisms of Recruitment and Functional Role. J. Immunol. 164: 4399-4407 [Abstract] [Full Text]
Korff, T., Augustin, H. G. (1998). Integration of Endothelial Cells in Multicellular Spheroids Prevents Apoptosis and Induces Differentiation. JCB 143: 1341-1352 [Abstract] [Full Text]
Gerli, R., Paolucci, C., Gresele, P., Bistoni, O., Fiorucci, S., Muscat, C., Belia, S., Bertotto, A., Costantini, V. (1998). Salicylates Inhibit Adhesion and Transmigration of T Lymphocytes by Preventing Integrin Activation Induced by Contact With Endothelial Cells. Blood 92: 2389-2398 [Abstract] [Full Text]
McMahon, G. A., Garfinkel, S., Prudovsky, I., Hu, X., Maciag, T. (1997). Intracellular Precursor Interleukin (IL)-1alpha , but Not Mature IL-1alpha , Is Able to Regulate Human Endothelial Cell Migration in Vitro. J. Biol. Chem. 272: 28202-28205 [Abstract] [Full Text]
Watanabe, K., Jaffe, E.A. (1997). Lipopolysaccharide suppresses thrombin- and histamine-stimulated rises in intracellular calcium concentration in human endothelial cells. Innate Immunity 4: 279-284 [Abstract]
Collard, C. D., Vakeva, A., Bukusoglu, C., Zund, G., Sperati, C. J., Colgan, S. P., Stahl, G. L. (1997). Reoxygenation of Hypoxic Human Umbilical Vein Endothelial Cells Activates the Classic Complement Pathway. Circulation 96: 326-333 [Abstract] [Full Text]
Lee, M.-J., Evans, M., Hla, T. (1996). The Inducible G Protein-coupled Receptor edg-1 Signals via the G(i)/Mitogen-activated Protein Kinase Pathway. J. Biol. Chem. 271: 11272-11279 [Abstract] [Full Text]
Maier, J., Voulalas, P, Roeder, D, Maciag, T (1990). Extension of the life-span of human endothelial cells by an interleukin-1 alpha antisense oligomer. Science 249: 1570-1574 [Abstract]
Vaccaro, P. S., Joseph, L. B., Titterington, L., Stephens, R. E. (1987). Methods for the Initiation and Maintenance of Human Endothelial Cell Culture. VASC ENDOVASCULAR SURG 21: 391-400 [Abstract]
Rothenberg, M., Owen, W. Jr, Silberstein, D., Soberman, R., Austen, K., Stevens, R. (1987). Eosinophils cocultured with endothelial cells have increased survival and functional properties. Science 237: 645-647 [Abstract]
Ginsburg, D, Handin, R., Bonthron, D., Donlon, T., Bruns, G., Latt, S., Orkin, S. (1985). Human von Willebrand factor (vWF): isolation of complementary DNA (cDNA) clones and chromosomal localization. Science 228: 1401-1406 [Abstract]
Jaye, M, McConathy, E, Drohan, W, Tong, B, Deuel, T, Maciag, T (1985). Modulation of the sis gene transcript during endothelial cell differentiation in vitro. Science 228: 882-885 [Abstract]
Niwa, Y., Sakane, T., Shingu, M., Yanagida, I., Komura, J., Miyachi, Y. (1985). Neutrophil-Generated Active Oxygens in Linear IgA Bullous Dermatosis. Arch Dermatol 121: 73-78 [Abstract]
Maciag, T, Mehlman, T, Friesel, R, Schreiber, A. (1984). Heparin binds endothelial cell growth factor, the principal endothelial cell mitogen in bovine brain. Science 225: 932-935 [Abstract]
Thornton, S., Mueller, S., Levine, E. (1983). Human endothelial cells: use of heparin in cloning and long-term serial cultivation. Science 222: 623-625 [Abstract]