The Journal of Cell Biology, Vol 91, 689-694, Copyright © 1981 by The Rockefeller University Press

ARTICLES

Effects of antibodies against dynein and tubulin on the stiffness of flagellar axonemes

M Okuno, DJ Asai, K Ogawa and CJ Brokaw

Antidynein antibodies, previously shown to inhibit flagellar oscillation and active sliding of axonemal microtubules, increase the bending resistance of axonemes measured under relaxing conditions, but not the bending resistance of axonemes measured under rigor conditions. These observations suggest that antidynein antibodies can stabilize rigor cross-bridges between outer-doublet microtubules, by interfering with ATP-induced cross-bridge detachment. Stabilization of a small number of cross-bridge appears to be sufficient to cause substantial inhibition of the frequency of flagellar oscillation. Antitubulin antibodies, previously shown to inhibit flagellar oscillation without inhibiting active sliding of axonemal microtubules, do not increase the static bending resistance of axonemes. However, we observed a viscoelastic effect, corresponding to a large increase in the immediate bending resistance. This immediate bending resistance increase may be sufficient to explain inhibition of flagellar oscillation; but several alternative explanations cannot yet be excluded.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Gingras, D., White, D., Garin, J., Multigner, L., Job, D., Cosson, J., Huitorel, P., Zingg, H., Dumas, F., Gagnon, C. (1996). Purification, Cloning, and Sequence Analysis of a Mr = 30,000 Protein from Sea Urchin Axonemes That Is Important for Sperm Motility. RELATIONSHIP OF THE PROTEIN TO A DYNEIN LIGHT CHAIN. J. Biol. Chem. 271: 12807-12813 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents