Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization

doi:10.1083/jcb.92.3.665

This Article

Full Text (PDF, 1064K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Lane, E. B.

Search for Related Content

PubMed

PubMed Citation

Articles by Lane, E. B.

Pubmed/NCBI databases

Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization

EB Lane

The tonofilament-associated protein antigens recognized in epithelial cells by a group of six monoclonal antibodies have been studied by immunofluorescence and gel immunoautoradiography. The monoclonal antibodies were generated against detergent insoluble cytoskeleton extracts from a cultured simple epithelium derived cell line, Ptk1 cells. They show various tissue specificities, and while they all recognize components at the low end of the molecular weight range for intermediate filament proteins, they confirm that single antibody species can react with multiple polypeptides of different molecular weights in the tonofilament complex. The monoclonal antibodies described here demonstrate the presence of a simple epithelium antigenic determinant associated with intermediate filaments that is not detectable in the specialized cells of squamous and keratinizing epithelia but can reappear in such cells after transformation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Villadsen, R., Fridriksdottir, A. J., Ronnov-Jessen, L., Gudjonsson, T., Rank, F., LaBarge, M. A., Bissell, M. J., Petersen, O. W. (2007). Evidence for a stem cell hierarchy in the adult human breast. JCB 177: 87-101 [Abstract] [Full Text]
Long, H. A., Boczonadi, V., McInroy, L., Goldberg, M., Maatta, A. (2006). Periplakin-dependent re-organisation of keratin cytoskeleton and loss of collective migration in keratin-8-downregulated epithelial sheets. J. Cell Sci. 119: 5147-5159 [Abstract] [Full Text]
Perez, A., Barco, R., Fernandez, I., Price-Schiavi, S. A., Carraway, K. L. (2003). PEA3 Transactivates the Muc4/Sialomucin Complex Promoter in Mammary Epithelial and Tumor Cells. J. Biol. Chem. 278: 36942-36952 [Abstract] [Full Text]
Karashima, T., Watt, F. M. (2003). Interaction of periplakin and envoplakin with intermediate filaments. J. Cell Sci. 115: 5027-5037 [Abstract] [Full Text]
Hudson, D. L., Guy, A. T., Fry, P., O'Hare, M. J., Watt, F. M., Masters, J. R.W. (2001). Epithelial Cell Differentiation Pathways in the Human Prostate: Identification of Intermediate Phenotypes by Keratin Expression. J. Histochem. Cytochem. 49: 271-278 [Abstract] [Full Text]
Illingworth, I. M., Kiszka, I., Bagley, S., Ireland, G. W., Garrod, D. R., Kimber, S. J. (2000). Desmosomes Are Reduced in the Mouse Uterine Luminal Epithelium During the Preimplantation Period of Pregnancy: A Mechanism for Facilitation of Implantation. Biol. Reprod. 63: 1764-1773 [Abstract] [Full Text]
DiColandrea, T., Karashima, T., Maatta, A., Watt, F. M. (2000). Subcellular Distribution of Envoplakin and Periplakin: Insights into Their Role as Precursors of the Epidermal Cornified Envelope. JCB 151: 573-586 [Abstract] [Full Text]
Smalley, M. J., Titley, J., Paterson, H., Perusinghe, N., Clarke, C., O'Hare, M. J. (1999). Differentiation of Separated Mouse Mammary Luminal Epithelial and Myoepithelial Cells Cultured on EHS Matrix Analyzed by Indirect Immunofluorescence of Cytoskeletal Antigens. J. Histochem. Cytochem. 47: 1513-1524 [Abstract] [Full Text]
Whitehouse, C., Burchell, J., Gschmeissner, S., Brockhausen, I., Lloyd, K. O., Taylor-Papadimitriou, J. (1997). A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2-based O-Glycans. JCB 137: 1229-1241 [Abstract] [Full Text]
Paramio, J., Casanova, M., Alonso, A, Jorcano, J. (1997). Keratin intermediate filament dynamics in cell heterokaryons reveals diverse behaviour of different keratins. J. Cell Sci. 110: 1099-1111 [Abstract]
Rugg, E L, McLean, W H, Lane, E B, Pitera, R, McMillan, J R, Dopping-Hepenstal, P J, Navsaria, H A, Leigh, I M, Eady, R A (1994). A functional "knockout" of human keratin 14.. Genes Dev. 8: 2563-2573 [Abstract]
Atherton, A., O'Hare, M., Buluwela, L, Titley, J, Monaghan, P, Paterson, H., Warburton, M., Gusterson, B. (1994). Ectoenzyme regulation by phenotypically distinct fibroblast sub-populations isolated from the human mammary gland. J. Cell Sci. 107: 2931-2939 [Abstract]
Dale, B. A., Salonen, J., Jones, A. H. (1990). New Approaches And Concepts in The Study of Differentiation of Oral Epithelia. CROBM 1: 167-190 [Abstract] [Full Text]
Tazawa, T., Ito, M., Fujiwara, H., Shimizu, N., Sato, Y. (1988). Immunologic Characteristics of Keratins in Extramammary Paget's Disease. Arch Dermatol 124: 1063-1068 [Abstract]