Immunocytochemical localization of galactosyltransferase in HeLa cells: codistribution with thiamine pyrophosphatase in trans-Golgi cisternae

doi:10.1083/jcb.93.1.223

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Immunocytochemical localization of galactosyltransferase in HeLa cells: codistribution with thiamine pyrophosphatase in trans-Golgi cisternae

J Roth and EG Berger

An affinity-purified, monospecific rabbit antibody against soluble human milk galactosyltransferase was used to localize the enzyme in HeLa cells by immunofluorescence and by the protein A-gold technique at the electron microscope level. Specific immunofluorescence was observed in a juxtanuclear cytoplasmic region which was identified, on immunostained thin sections of low-temperature Lowicryl K4M-embedded HeLa cells, as Golgi apparatus. Label by gold particles was limited to two to three trans cisternae of the Golgi apparatus, indicating a compartmentalization of galactosyltransferase in the cisternal stack. Combination of preembedding thiamine pyrophosphatase cytochemistry, with postembedding immunostaining for galactosyltransferase proved codistribution of the two enzymes. However, the acid phosphatase- positive, trans-most cisterna was negative for galactosyltransferase. The close topological association of both galactosyltransferase and thiamine pyrophosphatase (or nucleoside diphosphatase) suggests a concerted action of both enzymes in glycosylation.
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Guo, S., Sato, T., Shirane, K., Furukawa, K. (2001). Galactosylation of N-linked oligosaccharides by human {beta}-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells. Glycobiology 11: 813-820 [Abstract] [Full Text]
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Johannes, L., Tenza, D., Antony, C., Goud, B. (1997). Retrograde Transport of KDEL-bearing B-fragment of Shiga Toxin. J. Biol. Chem. 272: 19554-19561 [Abstract] [Full Text]
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Ivessa, N. E., De Lemos-Chiarandini, C., Gravotta, D., Sabatini, D. D., Kreibich, G. (1995). The Brefeldin A-induced Retrograde Transport from the Golgi Apparatus to the Endoplasmic Reticulum Depends on Calcium Sequestered to Intracellular Stores. J. Biol. Chem. 270: 25960-25967 [Abstract] [Full Text]
Rabouille, C, Hui, N, Hunte, F, Kieckbusch, R, Berger, E., Warren, G, Nilsson, T (1995). Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides. J. Cell Sci. 108: 1617-1627 [Abstract]
Conibear, E, Pearse, B. (1994). A chimera of the cytoplasmic tail of the mannose 6-phosphate/IGF-II receptor and lysozyme localizes to the TGN rather than prelysosomes where the bulk of the endogenous receptor is found. J. Cell Sci. 107: 923-932 [Abstract]
Thyberg, J., Moskalewski, S. (1992). Disorganization of the Golgi complex and the cytoplasmic microtubule system in CHO cells exposed to okadaic acid. J. Cell Sci. 103: 1167-1175 [Abstract]
Cluett, E. B., Brown, W. J. (1992). Adhesion of Golgi cisternae by proteinaceous interactions: intercisternal bridges as putative adhesive structures. J. Cell Sci. 103: 773-784 [Abstract]