Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum

doi:10.1083/jcb.93.1.97

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Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum

Y Fujiki, AL Hubbard, S Fowler and PB Lazarow

A rapid and simple method for the isolation of membranes from subcellular organelles is described. The procedure consists of diluting the organelles in ice-cold 100 mM Na2CO3 followed by centrifugation to pellet the membranes. Closed vesicles are converted to open membrane sheets, and content proteins and peripheral membrane proteins are released in soluble form. Here we document the method by applying it to various subfractions of a rat liver microsomal fraction, prepared by continuous density gradient centrifugation according to Beaufay et al. (1974, J. Cell Biol. 61:213-231). The results confirm and extend those of previous investigators on the distribution of enzymes and proteins among the membranes of the smooth and rough endoplasmic reticulum. In the accompanying paper (1982, J. Cell Biol. 93:103-110) the procedure is applied to peroxisomes and mitochondria.
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Curran, S. P., Leverich, E. P., Koehler, C. M., Larsen, P. L. (2004). Defective Mitochondrial Protein Translocation Precludes Normal Caenorhabditis elegans Development. J. Biol. Chem. 279: 54655-54662 [Abstract] [Full Text]
Brasaemle, D. L., Dolios, G., Shapiro, L., Wang, R. (2004). Proteomic Analysis of Proteins Associated with Lipid Droplets of Basal and Lipolytically Stimulated 3T3-L1 Adipocytes. J. Biol. Chem. 279: 46835-46842 [Abstract] [Full Text]
Ogino, T., Fukuda, H., Imajoh-Ohmi, S., Kohara, M., Nomoto, A. (2004). Membrane Binding Properties and Terminal Residues of the Mature Hepatitis C Virus Capsid Protein in Insect Cells. J. Virol. 78: 11766-11777 [Abstract] [Full Text]
Subramanian, V., Garcia, A., Sekowski, A., Brasaemle, D. L. (2004). Hydrophobic sequences target and anchor perilipin A to lipid droplets. J. Lipid Res. 45: 1983-1991 [Abstract] [Full Text]
Dunkley, T. P. J., Watson, R., Griffin, J. L., Dupree, P., Lilley, K. S. (2004). Localization of Organelle Proteins by Isotope Tagging (LOPIT). Mol. Cell. Proteomics 3: 1128-1134 [Abstract] [Full Text]
Youker, R. T., Walsh, P., Beilharz, T., Lithgow, T., Brodsky, J. L. (2004). Distinct Roles for the Hsp40 and Hsp90 Molecular Chaperones during Cystic Fibrosis Transmembrane Conductance Regulator Degradation in Yeast. Mol. Biol. Cell 15: 4787-4797 [Abstract] [Full Text]
Hood, J. L., Brooks, W. H., Roszman, T. L. (2004). Differential Compartmentalization of the Calpain/Calpastatin Network with the Endoplasmic Reticulum and Golgi Apparatus. J. Biol. Chem. 279: 43126-43135 [Abstract] [Full Text]
Doolman, R., Leichner, G. S., Avner, R., Roitelman, J. (2004). Ubiquitin Is Conjugated by Membrane Ubiquitin Ligase to Three Sites, including the N Terminus, in Transmembrane Region of Mammalian 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase: IMPLICATIONS FOR STEROL-REGULATED ENZYME DEGRADATION. J. Biol. Chem. 279: 38184-38193 [Abstract] [Full Text]
Leary, S. C., Kaufman, B. A., Pellecchia, G., Guercin, G.-H., Mattman, A., Jaksch, M., Shoubridge, E. A. (2004). Human SCO1 and SCO2 have independent, cooperative functions in copper delivery to cytochrome c oxidase. Hum Mol Genet 13: 1839-1848 [Abstract] [Full Text]
Schwer, B., Ren, S., Pietschmann, T., Kartenbeck, J., Kaehlcke, K., Bartenschlager, R., Yen, T. S. B., Ott, M. (2004). Targeting of Hepatitis C Virus Core Protein to Mitochondria through a Novel C-Terminal Localization Motif. J. Virol. 78: 7958-7968 [Abstract] [Full Text]