A dependent pathway of gene functions leading to chromosome segregation in Saccharomyces cerevisiae

doi:10.1083/jcb.94.3.718

This Article

	Full Text (PDF, 1077K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wood, J. S.
	Articles by Hartwell, L. H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wood, J. S.
	Articles by Hartwell, L. H.


Social Bookmarking

	What's this?

ARTICLES

A dependent pathway of gene functions leading to chromosome segregation in Saccharomyces cerevisiae

JS Wood and LH Hartwell

Methyl-benzimidazole-2-ylcarbamate (MBC) inhibits the mitotic cell cycle of Saccharomyces cerevisiae at a stage subsequent to DNA synthesis and before the completion of nuclear division (Quinlan, R. A., C. I. Pogson, and K, Gull, 1980, J Cell Sci., 46: 341-352). The step in the cell cycle that is sensitive to MBC inhibition was ordered to reciprocal shift experiments with respect to the step catalyzed by cdc gene products. Execution of the CDC7 step is required for the initiation of DNA synthesis and for completion of the MBC-sensitive step. Results obtained with mutants (cdc2, 6, 8, 9, and 21) defective in DNA replication and with an inhibitor of DNA replication (hydroxyurea) suggest that some DNA replication required for execution of the MBC-sensitive step but that the completion of replication is not. Of particular interest were mutants (cdc5, 13, 14, 15, 16, 17, and 23) that arrest cell division after DNA replication but before nuclear division since previous experiments had not been able to resolve the pathway of events in this part of the cell cycle. Execution of the CDC17 step was found to be a prerequisite for execution of the MBC- sensitive step; the CDC13, 16 and 23 steps are executed independently of the MBC-sensitive step; execution of the MBC-sensitive step is prerequisite for execution of the MBC-sensitive step; execution of the MBC-sensitive step is prerequisite for execution of the CDC14 and 23 steps. These results considerably extend the dependent pathway of events that constitute the cell cycle of S. cerevisiae.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Diamond, A. E., Park, J.-S., Inoue, I., Tachikawa, H., Neiman, A. M. (2009). The Anaphase Promoting Complex Targeting Subunit Ama1 Links Meiotic Exit to Cytokinesis during Sporulation in Saccharomyces cerevisiae. Mol. Biol. Cell 20: 134-145 [Abstract] [Full Text]
Jessberger, R. (2005). How To Divorce Engaged Chromosomes?. Mol. Cell. Biol. 25: 18-22 [Full Text]
Schollaert, K. L., Poisson, J. M., Searle, J. S., Schwanekamp, J. A., Tomlinson, C. R., Sanchez, Y. (2004). A Role for Saccharomyces cerevisiae Chk1p in the Response to Replication Blocks. Mol. Biol. Cell 15: 4051-4063 [Abstract] [Full Text]
Bartholomew, C. R., Woo, S. H., Chung, Y. S., Jones, C., Hardy, C. F. J. (2001). Cdc5 Interacts with the Wee1 Kinase in Budding Yeast. Mol. Cell. Biol. 21: 4949-4959 [Abstract] [Full Text]
Furstenthal, L., Kaiser, B. K., Swanson, C., Jackson, P. K. (2001). Cyclin E Uses Cdc6 as a Chromatin-Associated Receptor Required for DNA Replication. JCB 152: 1267-1278 [Abstract] [Full Text]
Khalfan, W., Ivanovska, I., Rose, M. D. (2000). Functional Interaction Between the PKC1 Pathway and CDC31 Network of SPB Duplication Genes. Genetics 155: 1543-1559 [Abstract] [Full Text]
Flattery-O'Brien, J. A., Dawes, I. W. (1998). Hydrogen Peroxide Causes RAD9-dependent Cell Cycle Arrest in G2 in Saccharomyces cerevisiae whereas Menadione Causes G1 Arrest Independent of RAD9 Function. J. Biol. Chem. 273: 8564-8571 [Abstract] [Full Text]
Taylor, G. S., Liu, Y., Baskerville, C., Charbonneau, H. (1997). The Activity of Cdc14p, an Oligomeric Dual Specificity Protein Phosphatase from Saccharomyces cerevisiae, Is Required for Cell Cycle Progression. J. Biol. Chem. 272: 24054-24063 [Abstract] [Full Text]
Ohkura, H, Hagan, I M, Glover, D M (1995). The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells.. Genes Dev. 9: 1059-1073 [Abstract]
Brown, M., Garvik, B., Hartwell, L., Kadyk, L., Seeley, T., Weinert, T. (1991). Fidelity of Mitotic Chromosome Transmission. Cold Spring Harb Symp Quant Biol 56: 359-365 [Abstract]
Weinert, T., Hartwell, L. (1988). The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae. Science 241: 317-322 [Abstract]