Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells

doi:10.1083/jcb.95.1.333

This Article

Full Text (PDF, 3487K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Greenburg, G.

Articles by Hay, E. D.

Search for Related Content

PubMed

PubMed Citation

Articles by Greenburg, G.

Articles by Hay, E. D.

Pubmed/NCBI databases

Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells

G Greenburg and ED Hay

This study of epithelial-mesenchymal transformation and epithelial cell polarity in vitro reveals that environmental conditions can have a profound effect on the epithelial phenotype, cell shape, and polarity as expressed by the presence of apical and basal surfaces. A number of different adult and embryonic epithelia were suspended within native collagen gels. Under these conditions, cells elongate, detach from the explants, and migrate as individual cells within the three-dimensional lattice, a previously unknown property of well-differentiated epithelia. Epithelial cells from adult and embryonic anterior lens were studied in detail. Elongated cells derived from the apical surface develop pseudopodia and filopodia characteristic of migratory cells and acquire a morphology and ultrastructure virtually indistinguishable from that of mesenchymal cells in vivo. It is concluded from these experiments that the three-dimensional collagen gel can promote dissociation, migration, and acquisition of secretory organelles by differentiated epithelial cells, and can abolish the apical-basal cell polarity characteristic of the original epithelium.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Ogawa, Y., Shimmura, S., Kawakita, T., Yoshida, S., Kawakami, Y., Tsubota, K. (2009). Epithelial Mesenchymal Transition in Human Ocular Chronic Graft-Versus-Host Disease. Am. J. Pathol. 175: 2372-2381 [Abstract] [Full Text]
Kolosionek, E., Savai, R., Ghofrani, H. A., Weissmann, N., Guenther, A., Grimminger, F., Seeger, W., Banat, G. A., Schermuly, R. T., Pullamsetti, S. S. (2009). Expression and Activity of Phosphodiesterase Isoforms during Epithelial Mesenchymal Transition: The Role of Phosphodiesterase 4. Mol. Biol. Cell 20: 4751-4765 [Abstract] [Full Text]
Hackett, T.-L., Warner, S. M., Stefanowicz, D., Shaheen, F., Pechkovsky, D. V., Murray, L. A., Argentieri, R., Kicic, A., Stick, S. M., Bai, T. R., Knight, D. A. (2009). Induction of Epithelial-Mesenchymal Transition in Primary Airway Epithelial Cells from Patients with Asthma by Transforming Growth Factor-{beta}1. Am. J. Respir. Crit. Care Med. 180: 122-133 [Abstract] [Full Text]
Aldehni, F., Spitzner, M., Martins, J. R., Barro-Soria, R., Schreiber, R., Kunzelmann, K. (2009). Bestrophin 1 Promotes Epithelial-to-mesenchymal Transition of Renal Collecting Duct Cells. J. Am. Soc. Nephrol. 20: 1556-1564 [Abstract] [Full Text]
Ahlstrom, J. D., Erickson, C. A. (2009). The neural crest epithelial-mesenchymal transition in 4D: a `tail' of multiple non-obligatory cellular mechanisms. Development 136: 1801-1812 [Abstract] [Full Text]
Guan, F., Handa, K., Hakomori, S.-i. (2009). Specific glycosphingolipids mediate epithelial-to-mesenchymal transition of human and mouse epithelial cell lines. Proc. Natl. Acad. Sci. USA 106: 7461-7466 [Abstract] [Full Text]
Yu, W., Ruest, L.-B., Svoboda, K. K. H. (2009). Regulation of Epithelial-Mesenchymal Transition in Palatal Fusion. Exp. Biol. Med. 234: 483-491 [Abstract] [Full Text]
Klymkowsky, M. W., Savagner, P. (2009). Epithelial-Mesenchymal Transition: A Cancer Researcher's Conceptual Friend and Foe. Am. J. Pathol. 174: 1588-1593 [Abstract] [Full Text]
Palmer, M. B., Majumder, P., Cooper, J. C., Yoon, H., Wade, P. A., Boss, J. M. (2009). Yin Yang 1 Regulates the Expression of Snail through a Distal Enhancer. Mol Cancer Res 7: 221-229 [Abstract] [Full Text]
Soltermann, A., Tischler, V., Arbogast, S., Braun, J., Probst-Hensch, N., Weder, W., Moch, H., Kristiansen, G. (2008). Prognostic Significance of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transition Protein Expression in Non-Small Cell Lung Cancer. Clin. Cancer Res. 14: 7430-7437 [Abstract] [Full Text]
Puglisi, F, Puppin, C, Pegolo, E, Andreetta, C, Pascoletti, G, D'Aurizio, F, Pandolfi, M, Fasola, G, Piga, A, Damante, G, Di Loreto, C (2008). Expression of periostin in human breast cancer. J. Clin. Pathol. 61: 494-498 [Abstract] [Full Text]
Dhasarathy, A., Kajita, M., Wade, P. A. (2007). The Transcription Factor Snail Mediates Epithelial to Mesenchymal Transitions by Repression of Estrogen Receptor-{alpha}. Mol. Endocrinol. 21: 2907-2918 [Abstract] [Full Text]
Willis, B. C., Borok, Z. (2007). TGF-beta-induced EMT: mechanisms and implications for fibrotic lung disease. Am. J. Physiol. Lung Cell. Mol. Physiol. 293: L525-L534 [Abstract] [Full Text]
Moss, L. G., Rhodes, C. J. (2007). {beta}-Cell Regeneration: Epithelial Mesenchymal Transition Pre-EMTpted by Lineage Tracing?. Diabetes 56: 281-282 [Full Text]
Strutz, F., Zeisberg, M. (2006). Renal Fibroblasts and Myofibroblasts in Chronic Kidney Disease. J. Am. Soc. Nephrol. 17: 2992-2998 [Full Text]
Wu, X., Chen, H., Parker, B., Rubin, E., Zhu, T., Lee, J. S., Argani, P., Sukumar, S. (2006). HOXB7, a Homeodomain Protein, Is Overexpressed in Breast Cancer and Confers Epithelial-Mesenchymal Transition. Cancer Res. 66: 9527-9534 [Abstract] [Full Text]
Kang, P., Svoboda, K.K.H. (2005). Epithelial-Mesenchymal Transformation during Craniofacial Development. JDR 84: 678-690 [Abstract] [Full Text]
Beningo, K. A., Dembo, M., Wang, Y.-l. (2004). Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors. Proc. Natl. Acad. Sci. USA 101: 18024-18029 [Abstract] [Full Text]
Casaroli-Marano, R. P., Pagan, R., Vilaro, S. (1999). Epithelial-Mesenchymal Transition in Proliferative Vitreoretinopathy: Intermediate Filament Protein Expression in Retinal Pigment Epithelial Cells. IOVS 40: 2062-2072 [Abstract] [Full Text]
Shuler, C. F. (1995). Programmed Cell Death and Cell Transformation in Craniofacial Development. CROBM 6: 202-217 [Abstract] [Full Text]
Aplin, J. D., Sattar, A., Mould, A. P. (1992). Variant choriocarcinoma (BeWo) cells that differ in adhesion and migration on fibronectin display conserved patterns of integrin expression. J. Cell Sci. 103: 435-444 [Abstract]
Diewert, V. M., Wang, K.-Y. (1992). Recent Advances in Primary Palate and Midface Morphogenesis Research. CROBM 4: 111-130 [Abstract] [Full Text]
Vidaurri-Leal, J., Hohman, R., Glaser, B. M. (1984). Effect of Vitreous on Morphologic Characteristics of Retinal Pigment Epithelial Cells: A New Approach to the Study of Proliferative Vitreoretinopathy. Arch Ophthalmol 102: 1220-1223 [Abstract]