Mitotic architecture of the cell: the filament networks of the nucleus and cytoplasm

doi:10.1083/jcb.96.3.896

This Article

	Full Text (PDF, 5590K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Capco, D. G.
	Articles by Penman, S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Capco, D. G.
	Articles by Penman, S.


Social Bookmarking

	What's this?

ARTICLES

Mitotic architecture of the cell: the filament networks of the nucleus and cytoplasm

DG Capco and S Penman

The skeletal framework of cells at the various stages of mitosis are prepared by extraction with nonionic detergent and examined by stereoscopic whole mount electron microscopy. The insoluble filament network remaining after the detergent-extraction and the depolymerization of microtubules is shown. The nonchromatin filament network of the nucleus, or nuclear matrix, becomes visible as the chromatin condenses at prophase. Filaments are associated with the chromosomes throughout mitosis. Parts of the chromosomes are associated with or are near the nuclear lamina at early stages. The nuclear lamina disappears at metaphase while chromosomes remain associated with filaments now continuous with the cytoplasmic network. Microtubules appear to be unnecessary for maintaining the chromosome position in these preparations since comparison of cells with and without microtubules shows no gross change in chromosome arrangement. The cellular filament network at metaphase and anaphase appears continuous from the plasma lamina to the chromosomes. The filament networks visualized here may be responsible for the prometaphase chromosome movement and participate in the formation of the midbody.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Javed, A., Barnes, G. L., Pratap, J., Antkowiak, T., Gerstenfeld, L. C., van Wijnen, A. J., Stein, J. L., Lian, J. B., Stein, G. S. (2005). Impaired intranuclear trafficking of Runx2 (AML3/CBFA1) transcription factors in breast cancer cells inhibits osteolysis in vivo. Proc. Natl. Acad. Sci. USA 102: 1454-1459 [Abstract] [Full Text]
Zaidi, S. K., Young, D. W., Pockwinse, S. M., Javed, A., Lian, J. B., Stein, J. L., van Wijnen, A. J., Stein, G. S. (2003). Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells. Proc. Natl. Acad. Sci. USA 100: 14852-14857 [Abstract] [Full Text]
Doornaert, B., Leblond, V., Galiacy, S., Gras, G., Planus, E., Laurent, V., Isabey, D., Lafuma, C. (2003). Negative impact of DEP exposure on human airway epithelial cell adhesion, stiffness, and repair. Am. J. Physiol. Lung Cell. Mol. Physiol. 284: L119-L132 [Abstract] [Full Text]
Goldman, R. D., Gruenbaum, Y., Moir, R. D., Shumaker, D. K., Spann, T. P. (2002). Nuclear lamins: building blocks of nuclear architecture. Genes Dev. 16: 533-547 [Full Text]
Nievers, M., Schaapveld, R., Oomen, L., Fontao, L, Geerts, D, Sonnenberg, A (1998). Ligand-independent role of the beta 4 integrin subunit in the formation of hemidesmosomes. J. Cell Sci. 111: 1659-1672 [Abstract]
Nievers, M., Birge, R., Greulich, H, Verkleij, A., Hanafusa, H, van Bergen en Henegouwen, P. (1997). v-Crk-induced cell transformation: changes in focal adhesion composition and signaling. J. Cell Sci. 110: 389-399 [Abstract]