Protein loss during nuclear isolation

doi:10.1083/jcb.97.4.1240

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Protein loss during nuclear isolation

PL Paine, CF Austerberry, LJ Desjarlais and SB Horowitz

Cryomicrodissection makes possible the measurement of the entire in vivo protein content of the amphibian oocyte nucleus and provides a heretofore missing baseline for estimating protein loss during nuclear isolation by other methods. When oocyte nuclei are isolated into an aqueous medium, they lose 95% of their protein with a half-time of 250 s. This result implies an even more rapid loss of protein from aqueously isolated nuclei of ordinary-size cells.
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