The Journal of Cell Biology, Vol 97, 1950-1953, Copyright © 1983 by The Rockefeller University Press

ARTICLES

In vitro induction of cartilage-specific macromolecules by a bone extract

SM Seyedin, AY Thompson, DM Rosen and KA Piez

An in vitro system has been developed to study the onset of chondrogenesis. Embryonic rat muscle mesenchymal cells, when treated in suspension culture with an extract of bovine bone matrix, synthesized cartilage-specific proteoglycan and type II collagen. The synthesis of these two macromolecules was assayed by the enzyme-linked immunosorbent assay inhibition technique. Further evidence of chondrogenesis was demonstrated by morphological changes of treated cells when cultured in firm agarose and stained for metachromatic matrix. Even with crude bone matrix extracts, the assay was sensitive at the microgram level and significant differences in cartilage macromolecules compared with controls were observed in 2-3 d. In vivo the same extract induced first cartilage and then bone.
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This article has been cited by other articles:

• Harada, K., Oida, S., Sasaki, S., Enomoto, S. (1990). Chondrocyte-like Colony Formation of Mesenchymal Cells by Dentin Extracts in Agarose Gel Culture. JDR 69: 1555-1559 [Abstract]  
• Kawai, T., Urist, M.R. (1989). Bovine Tooth-derived Bone Morphogenetic Protein. JDR 68: 1069-1074 [Abstract]  
• Inoue, T., Deporter, D.A., Melcher, A.H. (1986). Basic Biological Sciences Induction of Chondrogenesis in Muscle, Skin, Bone Marrow, and Periodontal Ligament by Demineralized Dentin and Bone Matrix in vivo and in vitro. JDR 65: 12-22 [Abstract]  

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