The Journal of Cell Biology, Vol 98, 347-351, Copyright © 1984 by The Rockefeller University Press

ARTICLES

Immunocytochemical demonstration of alpha-tubulin modification during axonal maturation in the cerebellar cortex

R Cumming, RD Burgoyne and NA Lytton

Previous light microscopic immunocytochemical studies using two monoclonal antibodies that recognise alpha-tubulin (YOL/34 and YL1/2) but differ in their isotypic specificity have shown that the unmyelinated parallel fiber axons in the cerebellar cortex are labeled with only one of the antibodies (YOL/34). We now show that at 10 d postnatally the parallel fibers are labeled with both antibodies, and that during development YL1/2 (but not YOL/34) immunoreactivity disappears progressively from parallel fibers in the lower regions of the molecular layer upwards towards the external germinal layer. By approximately 28 d postnatally, the differential staining pattern of parallel fibers by the antibodies is established throughout the molecular layer. The time course, light microscopic, and ultrastructural staining distribution corresponds to a progressive change in alpha-tubulin immunoreactivity as the parallel fibers form synaptic contacts. This modification of alpha-tubulin (which was not observed in Purkinje cell dendrites or Bergmann glia) may be related to the formation of a basic isotype of alpha-tubulin within parallel fiber axons at maturation.
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This article has been cited by other articles:

• Paturle-Lafanechere, L, Manier, M, Trigault, N, Pirollet, F, Mazarguil, H, Job, D (1994). Accumulation of delta 2-tubulin, a major tubulin variant that cannot be tyrosinated, in neuronal tissues and in stable microtubule assemblies. J. Cell Sci. 107: 1529-1543 [Abstract]  
• Reichardt, L. (1984). Immunological approaches to the nervous system. Science 225: 1294-1299 [Abstract]  

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