The Journal of Cell Biology, Vol 98, 1090-1097, Copyright © 1984 by The Rockefeller University Press

ARTICLES

Studies on the expression of the microtubule-associated protein, tau, during mouse brain development, with newly isolated complementary DNA probes

DG Drubin, D Caput and MW Kirschner

Tau protein is a collection of closely related polypeptides that associate with microtubules in vivo and stimulate their assembly in vitro. Using an affinity-purified antiserum against bovine brain tau protein, we found that the number and amount of tau polypeptides changes dramatically during mouse brain development. The different forms appear to result from changes in tau mRNA since in vitro translation products reflect the qualitative and quantitative changes found in vivo. To study the mRNA and genomic complexity of tau protein, we used tau mRNA, purified from polysomes with tau antiserum, to isolate embryonic mouse tau complementary DNA clones. With these probes we have determined that embryonic tau protein is translated from a 6-kb mRNA that persists throughout brain development.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Dawson, H. N., Cantillana, V., Chen, L., Vitek, M. P. (2007). The Tau N279K Exon 10 Splicing Mutation Recapitulates Frontotemporal Dementia and Parkinsonism Linked to Chromosome 17 Tauopathy in a Mouse Model. J. Neurosci. 27: 9155-9168 [Abstract] [Full Text]  
• Tokuraku, K., Matsushima, K., Matui, T., Nakagawa, H., Katsuki, M., Majima, R., Kotani, S. (2003). The Number of Repeat Sequences in Microtubule-associated Protein 4 Affects the Microtubule Surface Properties. J. Biol. Chem. 278: 29609-29618 [Abstract] [Full Text]  
• Lee, G, Cowan, N, Kirschner, M (1988). The primary structure and heterogeneity of tau protein from mouse brain. Science 239: 285-288 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents