Relationship of actin and tubulin distribution to bud growth in wild- type and morphogenetic-mutant Saccharomyces cerevisiae

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Relationship of actin and tubulin distribution to bud growth in wild- type and morphogenetic-mutant Saccharomyces cerevisiae

AE Adams and JR Pringle

The distribution of actin in wild-type cells and in morphogenetic mutants of the budding yeast Saccharomyces cerevisiae was explored by staining cells with fluorochrome-labeled phallotoxins after fixing and permeabilizing the cells by several methods. The actin appeared to be localized in a set of cortical spots or patches, as well as in a network of cytoplasmic fibers. Bundles of filaments that may possibly correspond to the fibers visualized by fluorescence were observed with the electron microscope. The putative actin spots were concentrated in small and medium-sized buds and at what were apparently the sites of incipient bud formation on unbudded cells, whereas the putative actin fibers were generally oriented along the long axes of the mother-bud pairs. In several morphogenetic mutants that form multiple, abnormally elongated buds, the actin patches were conspicuously clustered at the tips of most buds, and actin fibers were clearly oriented along the long axes of the buds. There was a strong correlation between the occurrence of active growth at particular bud tips and clustering of actin spots at those same tips. Near the end of the cell cycle in wild- type cells, actin appeared to concentrate (as a cluster of spots or a band) in the neck region connecting the mother cell to its bud. Observations made using indirect immunofluorescence with a monoclonal anti-yeast-tubulin antibody on the morphogenetic mutant cdc4 (which forms multiple, abnormally elongated buds while the nuclear cycle is arrested) revealed the surprising occurrence of multiple bundles of cytoplasmic microtubules emanating from the one duplicated spindle-pole body per cell. It seems that most or all of the buds contain one or more of these bundles of microtubules, which often can be seen to extend to the very tips of the buds. These observations are consistent with the hypotheses that actin, tubulin, or both may be involved in the polarization of growth and localization of cell-wall deposition that occurs during the yeast cell cycle.
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Martin, S. W., Konopka, J. B. (2004). SUMO Modification of Septin-interacting Proteins in Candida albicans. J. Biol. Chem. 279: 40861-40867 [Abstract] [Full Text]
Young, M. E., Cooper, J. A., Bridgman, P. C. (2004). Yeast actin patches are networks of branched actin filaments. JCB 166: 629-635 [Abstract] [Full Text]
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Huang, B., Zeng, G., Ng, A. Y.J., Cai, M. (2003). Identification of Novel Recognition Motifs and Regulatory Targets for the Yeast Actin-regulating Kinase Prk1p. Mol. Biol. Cell 14: 4871-4884 [Abstract] [Full Text]
Nekrasov, V. S., Smith, M. A., Peak-Chew, S., Kilmartin, J. V. (2003). Interactions between Centromere Complexes in Saccharomyces cerevisiae. Mol. Biol. Cell 14: 4931-4946 [Abstract] [Full Text]
deHart, A. K.A., Schnell, J. D., Allen, D. A., Tsai, J.-Y., Hicke, L. (2003). Receptor Internalization in Yeast Requires the Tor2-Rho1 Signaling Pathway. Mol. Biol. Cell 14: 4676-4684 [Abstract] [Full Text]
Alic, N., Higgins, V. J., Pichova, A., Breitenbach, M., Dawes, I. W. (2003). Lipid Hydroperoxides Activate the Mitogen-activated Protein Kinase Mpk1p in Saccharomyces cerevisiae. J. Biol. Chem. 278: 41849-41855 [Abstract] [Full Text]
Caviston, J. P., Longtine, M., Pringle, J. R., Bi, E. (2003). The Role of Cdc42p GTPase-activating Proteins in Assembly of the Septin Ring in Yeast. Mol. Biol. Cell 14: 4051-4066 [Abstract] [Full Text]
Warenda, A. J., Kauffman, S., Sherrill, T. P., Becker, J. M., Konopka, J. B. (2003). Candida albicans Septin Mutants Are Defective for Invasive Growth and Virulence. Infect. Immun. 71: 4045-4051 [Abstract] [Full Text]
Scott, K. L., Plon, S. E. (2003). Loss of Sin3/Rpd3 Histone Deacetylase Restores the DNA Damage Response in Checkpoint-Deficient Strains of Saccharomyces cerevisiae. Mol. Cell. Biol. 23: 4522-4531 [Abstract] [Full Text]
Dong, Y., Pruyne, D., Bretscher, A. (2003). Formin-dependent actin assembly is regulated by distinct modes of Rho signaling in yeast. JCB 161: 1081-1092 [Abstract] [Full Text]
Gourlay, C. W., Dewar, H., Warren, D. T., Costa, R., Satish, N., Ayscough, K. R. (2003). An interaction between Sla1p and Sla2p plays a role in regulating actin dynamics and endocytosis in budding yeast. J. Cell Sci. 116: 2551-2564 [Abstract] [Full Text]
Toi, H., Fujimura-Kamada, K., Irie, K., Takai, Y., Todo, S., Tanaka, K. (2003). She4p/Dim1p Interacts with the Motor Domain of Unconventional Myosins in the Budding Yeast, Saccharomyces cerevisiae. Mol. Biol. Cell 14: 2237-2249 [Abstract] [Full Text]
Bretscher, A. (2003). Polarized growth and organelle segregation in yeast: the tracks, motors, and receptors. JCB 160: 811-816 [Abstract] [Full Text]
McCleland, M. L., Gardner, R. D., Kallio, M. J., Daum, J. R., Gorbsky, G. J., Burke, D. J., Stukenberg, P. T. (2003). The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 17: 101-114 [Abstract] [Full Text]
Liu, J.-L., Sung, L.-Y., Tian, X. C., Yang, X. (2002). Hypertonicity-Induced Projections Reflect Cell Polarity in Mouse Metaphase II Oocytes: Involvement of Microtubules, Microfilaments, and Chromosomes. Biol. Reprod. 67: 1853-1863 [Abstract] [Full Text]
Lee, P. R., Song, S., Ro, H.-S., Park, C. J., Lippincott, J., Li, R., Pringle, J. R., De Virgilio, C., Longtine, M. S., Lee, K. S. (2002). Bni5p, a Septin-Interacting Protein, Is Required for Normal Septin Function and Cytokinesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 6906-6920 [Abstract] [Full Text]
Henry, K. R., D'Hondt, K., Chang, J., Newpher, T., Huang, K., Hudson, R. T., Riezman, H., Lemmon, S. K. (2002). Scd5p and Clathrin Function Are Important for Cortical Actin Organization, Endocytosis, and Localization of Sla2p in Yeast. Mol. Biol. Cell 13: 2607-2625 [Abstract] [Full Text]
Warenda, A. J., Konopka, J. B. (2002). Septin Function in Candida albicans Morphogenesis. Mol. Biol. Cell 13: 2732-2746 [Abstract] [Full Text]
Pruyne, D., Evangelista, M., Yang, C., Bi, E., Zigmond, S., Bretscher, A., Boone, C. (2002). Role of Formins in Actin Assembly: Nucleation and Barbed-End Association. Science 297: 612-615 [Abstract] [Full Text]
Guertin, D. A., Trautmann, S., McCollum, D. (2002). Cytokinesis in Eukaryotes. Microbiol. Mol. Biol. Rev. 66: 155-178 [Abstract] [Full Text]
Chai, B., Hsu, J.-m., Du, J., Laurent, B. C. (2002). Yeast RSC Function Is Required for Organization of the Cellular Cytoskeleton via an Alternative PKC1 Pathway. Genetics 161: 575-584 [Abstract] [Full Text]
Warren, D. T., Andrews, P. D., Gourlay, C. W., Ayscough, K. R. (2002). Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics. J. Cell Sci. 115: 1703-1715 [Abstract] [Full Text]
Oberholzer, U., Marcil, A., Leberer, E., Thomas, D. Y., Whiteway, M. (2002). Myosin I Is Required for Hypha Formation in Candidaalbicans. Eukaryot Cell 1: 213-228 [Abstract] [Full Text]
Zhu, W., Rainville, I. R., Ding, M., Bolus, M., Heintz, N. H., Pederson, D. S. (2002). Evidence That the pre-mRNA Splicing Factor Clf1p Plays a Role in DNA Replication in Saccharomyces cerevisiae. Genetics 160: 1319-1333 [Abstract] [Full Text]
Mochida, J., Yamamoto, T., Fujimura-Kamada, K., Tanaka, K. (2002). The Novel Adaptor Protein, Mti1p, and Vrp1p, a Homolog of Wiskott-Aldrich Syndrome Protein-Interacting Protein (WIP), May Antagonistically Regulate Type I Myosins in Saccharomyces cerevisiae. Genetics 160: 923-934 [Abstract] [Full Text]
Cooper, K. F., Strich, R. (2002). Saccharomycescerevisiae C-Type Cyclin Ume3p/Srb11p Is Required for Efficient Induction and Execution of Meiotic Development. Eukaryot Cell 1: 66-74 [Abstract] [Full Text]
Yang, H.-C., Pon, L. A. (2002). Actin cable dynamics in budding yeast. Proc. Natl. Acad. Sci. USA 99: 751-756 [Abstract] [Full Text]
Schmitz, H.-P., Huppert, S., Lorberg, A., Heinisch, J. J. (2002). Rho5p downregulates the yeast cell integrity pathway. J. Cell Sci. 115: 3139-3148 [Abstract] [Full Text]
Hoepfner, D., van den Berg, M., Philippsen, P., Tabak, H. F., Hettema, E. H. (2001). A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae. JCB 155: 979-990 [Abstract] [Full Text]
Zeng, G., Yu, X., Cai, M. (2001). Regulation of Yeast Actin Cytoskeleton-Regulatory Complex Pan1p/Sla1p/End3p by Serine/Threonine Kinase Prk1p. Mol. Biol. Cell 12: 3759-3772 [Abstract] [Full Text]
Beach, D. L., Bloom, K. (2001). ASH1 mRNA Localization in Three Acts. Mol. Biol. Cell 12: 2567-2577 [Abstract] [Full Text]
Harkins, H. A., Page, N., Schenkman, L. R., De Virgilio, C., Shaw, S., Bussey, H., Pringle, J. R. (2001). Bud8p and Bud9p, Proteins That May Mark the Sites for Bipolar Budding in Yeast. Mol. Biol. Cell 12: 2497-2518 [Abstract] [Full Text]
Ni, L., Snyder, M. (2001). A Genomic Study of the Bipolar Bud Site Selection Pattern in Saccharomyces cerevisiae. Mol. Biol. Cell 12: 2147-2170 [Abstract] [Full Text]
Ho, J., Bretscher, A. (2001). Ras Regulates the Polarity of the Yeast Actin Cytoskeleton through the Stress Response Pathway. Mol. Biol. Cell 12: 1541-1555 [Abstract] [Full Text]
Gladfelter, A. S., Moskow, J. J., Zyla, T. R., Lew, D. J. (2001). Isolation and Characterization of Effector-Loop Mutants of CDC42 in Yeast. Mol. Biol. Cell 12: 1239-1255 [Abstract] [Full Text]
Motegi, F., Arai, R., Mabuchi, I. (2001). Identification of Two Type V Myosins in Fission Yeast, One of Which Functions in Polarized Cell Growth and Moves Rapidly in the Cell. Mol. Biol. Cell 12: 1367-1380 [Abstract] [Full Text]
Gardner, R. D., Poddar, A., Yellman, C., Tavormina, P. A., Monteagudo, M. C., Burke, D. J. (2001). The Spindle Checkpoint of the Yeast Saccharomyces cerevisiae Requires Kinetochore Function and Maps to the CBF3 Domain. Genetics 157: 1493-1502 [Abstract] [Full Text]
Boldogh, I. R., Yang, H.-C., Nowakowski, W. D., Karmon, S. L., Hays, L. G., Yates, J. R. III, Pon, L. A. (2001). Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast. Proc. Natl. Acad. Sci. USA 98: 3162-3167 [Abstract] [Full Text]
Asleson, C. M., Bensen, E. S., Gale, C. A., Melms, A.-S., Kurischko, C., Berman, J. (2001). Candida albicans INT1-Induced Filamentation in Saccharomyces cerevisiae Depends on Sla2p. Mol. Cell. Biol. 21: 1272-1284 [Abstract] [Full Text]
Ozaki-Kuroda, K., Yamamoto, Y., Nohara, H., Kinoshita, M., Fujiwara, T., Irie, K., Takai, Y. (2001). Dynamic Localization and Function of Bni1p at the Sites of Directed Growth in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 827-839 [Abstract] [Full Text]
Ooms, L. M., McColl, B. K., Wiradjaja, F., Wijayaratnam, A. P. W., Gleeson, P., Gething, M. J., Sambrook, J., Mitchell, C. A. (2000). The Yeast Inositol Polyphosphate 5-Phosphatases Inp52p and Inp53p Translocate to Actin Patches following Hyperosmotic Stress: Mechanism for Regulating Phosphatidylinositol 4,5-Bisphosphate at Plasma Membrane Invaginations. Mol. Cell. Biol. 20: 9376-9390 [Abstract] [Full Text]
Sheu, Y.-J., Barral, Y., Snyder, M. (2000). Polarized Growth Controls Cell Shape and Bipolar Bud Site Selection in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 5235-5247 [Abstract] [Full Text]
Bachewich, C, Heath, I. (2000). Radial F-actin arrays precede new hypha formation in Saprolegnia: implications for establishing polar growth and regulating tip morphogenesis. J. Cell Sci. 111: 2005-2016 [Abstract]
Longtine, M. S., Theesfeld, C. L., McMillan, J. N., Weaver, E., Pringle, J. R., Lew, D. J. (2000). Septin-Dependent Assembly of a Cell Cycle-Regulatory Module in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 4049-4061 [Abstract] [Full Text]
Hurtado, C. A. R., Beckerich, J.-M., Gaillardin, C., Rachubinski, R. A. (2000). A Rac Homolog Is Required for Induction of Hyphal Growth in the Dimorphic Yeast Yarrowia lipolytica. J. Bacteriol. 182: 2376-2386 [Abstract] [Full Text]
Velmurugan, S., Yang, X.-M., Chan, C. S.-M., Dobson, M., Jayaram, M. (2000). Partitioning of the 2-{micro}m Circle Plasmid of Saccharomyces cerevisiae: Functional Coordination with Chromosome Segregation and Plasmid-Encoded Rep Protein Distribution. JCB 149: 553-566 [Abstract] [Full Text]
Karpova, T. S., Reck-Peterson, S. L., Elkind, N. B., Mooseker, M. S., Novick, P. J., Cooper, J. A. (2000). Role of Actin and Myo2p in Polarized Secretion and Growth of Saccharomyces cerevisiae. Mol. Biol. Cell 11: 1727-1737 [Abstract] [Full Text]
Elkind, N. B., Walch-Solimena, C., Novick, P. J. (2000). The Role of the Cooh Terminus of Sec2p in the Transport of Post-Golgi Vesicles. JCB 149: 95-110 [Abstract] [Full Text]
Bloecher, A., Tatchell, K. (2000). Dynamic Localization of Protein Phosphatase Type 1 in the Mitotic Cell Cycle of Saccharomyces cerevisiae. JCB 149: 125-140 [Abstract] [Full Text]
Schaerer-Brodbeck, C., Riezman, H. (2000). Functional Interactions between the p35 Subunit of the Arp2/3 Complex and Calmodulin in Yeast. Mol. Biol. Cell 11: 1113-1127 [Abstract] [Full Text]
Bi, E., Chiavetta, J. B., Chen, H., Chen, G.-C., Chan, C. S. M., Pringle, J. R. (2000). Identification of Novel, Evolutionarily Conserved Cdc42p-interacting Proteins and of Redundant Pathways Linking Cdc24p and Cdc42p to Actin Polarization in Yeast. Mol. Biol. Cell 11: 773-793 [Abstract] [Full Text]
Schaerer-Brodbeck, C, Riezman, H (2000). Saccharomyces cerevisiae Arc35p works through two genetically separable calmodulin functions to regulate the actin and tubulin cytoskeletons. J. Cell Sci. 113: 521-532 [Abstract]
Tang, H.-Y., Xu, J., Cai, M. (2000). Pan1p, End3p, and Sla1p, Three Yeast Proteins Required for Normal Cortical Actin Cytoskeleton Organization, Associate with Each Other and Play Essential Roles in Cell Wall Morphogenesis. Mol. Cell. Biol. 20: 12-25 [Abstract] [Full Text]
Kozminski, K. G., Chen, A. J., Rodal, A. A., Drubin, D. G. (2000). Functions and Functional Domains of the GTPase Cdc42p. Mol. Biol. Cell 11: 339-354 [Abstract] [Full Text]
Schott, D., Ho, J., Pruyne, D., Bretscher, A. (1999). The Cooh-Terminal Domain of Myo2p, a Yeast Myosin V, Has a Direct Role in Secretory Vesicle Targeting. JCB 147: 791-808 [Abstract] [Full Text]
Delley, P.-A., Hall, M. N. (1999). Cell Wall Stress Depolarizes Cell Growth via Hyperactivation of Rho1. JCB 147: 163-174 [Abstract] [Full Text]
McMillan, J. N., Longtine, M. S., Sia, R. A. L., Theesfeld, C. L., Bardes, E. S. G., Pringle, J. R., Lew, D. J. (1999). The Morphogenesis Checkpoint in Saccharomyces cerevisiae: Cell Cycle Control of Swe1p Degradation by Hsl1p and Hsl7p. Mol. Cell. Biol. 19: 6929-6939 [Abstract] [Full Text]
Theesfeld, C. L., Irazoqui, J. E., Bloom, K., Lew, D. J. (1999). The Role of Actin in Spindle Orientation Changes during the Saccharomyces cerevisiae Cell Cycle. JCB 146: 1019-1032 [Abstract] [Full Text]
Rodal, A. A., Tetreault, J. W., Lappalainen, P., Drubin, D. G., Amberg, D. C. (1999). Aip1p Interacts with Cofilin to Disassemble Actin Filaments. JCB 145: 1251-1264 [Abstract] [Full Text]
Sanders, S. L., Gentzsch, M., Tanner, W., Herskowitz, I. (1999). O-Glycosylation of Axl2/Bud10p by Pmt4p Is Required for Its Stability, Localization, and Function in Daughter Cells. JCB 145: 1177-1188 [Abstract] [Full Text]