Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression

doi:10.1083/jcb.98.4.1388

This Article

	Full Text (PDF, 1630K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Eichner, R.
	Articles by Sun, T. T.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Eichner, R.
	Articles by Sun, T. T.


Social Bookmarking

	What's this?

ARTICLES

Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression

R Eichner, P Bonitz and TT Sun

Human epidermal keratinocytes express under various growth conditions a total of at least nine keratins that can be divided into two subfamilies. Subfamily A comprises 40-, 46-, 48-, 50-/50'-, and 56.5- kilodalton (kd) keratins which are relatively acidic (pI less than 5.5) and, with the exception of 46-kd keratin, are recognized by AE1 monoclonal antibody. Subfamily B comprises 52-, 56-, 58-, and 65-67-kd keratins which are relatively basic (pI greater than 6) and are recognized by AE3 monoclonal antibody. Within each keratin subfamily, there is a constant member (50-/50'- and 58-kd keratins of the subfamilies A and B, respectively) that is always expressed. The other seven keratins of both subfamilies are variable members whose expression depends upon the cellular differentiated state, which is in turn modulated by the growth environment. The 56.5-kd keratin (subfamily A) and the 65-67-kd keratins (subfamily B) are coordinately expressed during keratinization. In contrast, the 40-, 46-, and 48-kd keratins (subfamily A) and the 52- and 56-kd keratins (subfamily B) are characteristic of cultured epidermal cells forming nonkeratinized colonies. These results demonstrate that human epidermal keratins can be classified according to their reactivity with monoclonal antikeratin antibodies, isoelectric point, and mode of expression. The classification of keratins into various subgroups may have important implications for the mechanisms of epidermal differentiation, the evolution of keratin heterogeneity, and the use of keratin markers for tumor diagnosis.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Sassi, F., Sarli, G., Brunetti, B., Morandi, F., Benazzi, C. (2008). Immunohistochemical characterization of mammary squamous cell carcinoma of the dog. jvdi 20: 766-773 [Abstract] [Full Text]
Sekiyama, E., Nakamura, T., Cooper, L. J., Kawasaki, S., Hamuro, J., Fullwood, N. J., Kinoshita, S. (2006). Unique distribution of thrombospondin-1 in human ocular surface epithelium.. IOVS 47: 1352-1358 [Abstract] [Full Text]
Liang, F.-X., Bosland, M. C., Huang, H., Romih, R., Baptiste, S., Deng, F.-M., Wu, X.-R., Shapiro, E., Sun, T.-T. (2005). Cellular basis of urothelial squamous metaplasia: roles of lineage heterogeneity and cell replacement. JCB 171: 835-844 [Abstract] [Full Text]
Wu, R., Abramson, A. L., Shikowitz, M. J., Dannenberg, A. J., Steinberg, B. M. (2005). Epidermal Growth Factor-Induced Cyclooxygenase-2 Expression Is Mediated through Phosphatidylinositol-3 Kinase, Not Mitogen-Activated Protein/Extracellular Signal-Regulated Kinase Kinase, in Recurrent Respiratory Papillomas. Clin. Cancer Res. 11: 6155-6161 [Abstract] [Full Text]
Nakamura, T., Endo, K.-I., Cooper, L. J., Fullwood, N. J., Tanifuji, N., Tsuzuki, M., Koizumi, N., Inatomi, T., Sano, Y., Kinoshita, S. (2003). The Successful Culture and Autologous Transplantation of Rabbit Oral Mucosal Epithelial Cells on Amniotic Membrane. IOVS 44: 106-116 [Abstract] [Full Text]
Nakamura, T., Nishida, K., Dota, A., Kinoshita, S. (2002). Changes in Conjunctival Clusterin Expression in Severe Ocular Surface Disease. IOVS 43: 1702-1707 [Abstract] [Full Text]
Nakamura, T., Nishida, K., Dota, A., Matsuki, M., Yamanishi, K., Kinoshita, S. (2001). Elevated Expression of Transglutaminase 1 and Keratinization-Related Proteins in Conjunctiva in Severe Ocular Surface Disease. IOVS 42: 549-556 [Abstract] [Full Text]
Wildi, S., Kleeff, J., Maruyama, H., Maurer, C. A., Friess, H., Buchler, M. W., Lander, A. D., Korc, M. (1999). Characterization of Cytokeratin 20 Expression in Pancreatic and Colorectal Cancer. Clin. Cancer Res. 5: 2840-2847 [Abstract] [Full Text]
Johnston, D., Hall, H., DiLorenzo, T. P., Steinberg, B. M. (1999). Elevation of the Epidermal Growth Factor Receptor and Dependent Signaling in Human Papillomavirus-infected Laryngeal Papillomas. Cancer Res. 59: 968-974 [Abstract] [Full Text]
Manabe, M., Lim, H. W., Winzer, M., Loomis, C. A. (1999). Architectural Organization of Filiform Papillae in Normal and Black Hairy Tongue Epithelium: Dissection of Differentiation Pathways in a Complex Human Epithelium According to Their Patterns of Keratin Expression. Arch Dermatol 135: 177-181 [Abstract] [Full Text]
Porter, R. M., Hutcheson, A. M., Rugg, E. L., Quinlan, R. A., Lane, E. B. (1998). cDNA Cloning, Expression, and Assembly Characteristics of Mouse Keratin 16. J. Biol. Chem. 273: 32265-32272 [Abstract] [Full Text]
Pankov, R, Simcha, I, Zoller, M, Oshima, R., Ben-Ze'ev, A (1997). Contrasting effects of K8 and K18 on stabilizing K19 expression, cell motility and tumorigenicity in the BSp73 adenocarcinoma. J. Cell Sci. 110: 965-974 [Abstract]
Vanderhooft, S. L., Francis, J. S., Holbrook, K. A., Dale, B. A., Fleckman, P. (1995). Familial Pityriasis Rubra Pilaris. Arch Dermatol 131: 448-453 [Abstract]
Nirunsuksiri, W., Presland, R. B., Brumbaugh, S. G., Dale, B. A., Fleckman, P. (1995). Decreased Profilaggrin Expression in Ichthyosis Vulgaris Is a Result of Selectively Impaired Posttranscriptional Control. J. Biol. Chem. 270: 871-876 [Abstract] [Full Text]
Baricault, L, de Nechaud, B, Sapin, C, Codogno, P, Denoulet, P, Trugnan, G (1994). The network organization and the phosphorylation of cytokeratins are concomitantly modified by forskolin in the enterocyte-like differentiated Caco-2 cell line. J. Cell Sci. 107: 2909-2918 [Abstract]
Castro-Munozledo, F (1994). Development of a spontaneous permanent cell line of rabbit corneal epithelial cells that undergoes sequential stages of differentiation in cell culture. J. Cell Sci. 107: 2343-2351 [Abstract]
Wu, R., Galvin, S, Wu, S., Xu, C, Blumenberg, M, Sun, T. (1993). A 300 bp 5'-upstream sequence of a differentiation-dependent rabbit K3 keratin gene can serve as a keratinocyte-specific promoter. J. Cell Sci. 105: 303-316 [Abstract]
Pang, Y., Schermer, A, Yu, J, Sun, T. (1993). Suprabasal change and subsequent formation of disulfide-stabilized homo- and hetero-dimers of keratins during esophageal epithelial differentiation. J. Cell Sci. 104: 727-740 [Abstract]
Tang, T., Hong, T., Lin, C., Lai, M., Liu, C., Lo, H., Wang, M., Chen, L., Chen, W., Ip, W, et, al. (1993). Nuclear proteins of the bovine esophageal epithelium. I. Monoclonal antibody W2 specifically reacts with condensed nuclei of differentiated superficial cells. J. Cell Sci. 104: 237-247 [Abstract]
Steinberg, B. M., Meade, R., Kalinowski, S., Abramson, A. L. (1990). Abnormal Differentiation of Human Papillomavirus-Induced Laryngeal Papillomas. Arch Otolaryngol Head Neck Surg 116: 1167-1171 [Abstract]
Vennix, P. P. C. A., Kuijpers, W., Tonnaer, E. L. C. M., Peters, T. A., Ramaekers, F. C. S. (1990). Cytokeratins in Induced Epidermoid Formations and Cholesteatoma Lesions. Arch Otolaryngol Head Neck Surg 116: 560-565 [Abstract]
Dale, B. A., Salonen, J., Jones, A. H. (1990). New Approaches And Concepts in The Study of Differentiation of Oral Epithelia. CROBM 1: 167-190 [Abstract] [Full Text]
Kopan, R, Fuchs, E (1989). A new look into an old problem: keratins as tools to investigate determination, morphogenesis, and differentiation in skin.. Genes Dev. 3: 1-15 [Abstract]