A tumor promoter induces rapid and coordinated reorganization of actin and vinculin in cultured cells

doi:10.1083/jcb.99.3.1045

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12-O-TETRADECANOYLPHORBOL-13-ACETATE

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A tumor promoter induces rapid and coordinated reorganization of actin and vinculin in cultured cells

M Schliwa, T Nakamura, KR Porter and U Euteneuer

Treatment of epithelial African green monkey kidney (BSC-1) cells with the potent tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a rapid and reversible redistribution of actin and vinculin that is detectable after only 2 min of treatment. Within 20-40 min, stress fibers disappear, while at the same time large actin-containing ribbons resembling ruffles develop both at the cell periphery and in more central regions. Vinculin is associated with these actin ribbons or bands in a punctate or patchy staining pattern. Adhesion to the substratum is changed from predominantly focal contacts associated with stress fiber ends in untreated cells to broad zones of close contact after TPA treatment. High voltage electron microscopic observations disclose the ribbons to consist of highly cross-linked actin filament networks. Thus, association of vinculin with filament networks, rather than (the ends of) filament bundles, is demonstrated. The integrity of microtubules and vimentin filaments is not affected by TPA treatment, but their distribution is altered to conform with the highly distorted cell shape. The response to TPA is neither prevented nor modified by nocodazole-induced depolymerization or taxol-induced stabilization of microtubules. An intact intermediate filament network seems not required either since colcemid-induced collapse of vimentin filaments towards the nucleus does not affect the cell's response to TPA. Rapid redistribution of actin and vinculin also takes place in enucleated cells and in the presence of cycloheximide, but is prevented by dinitrophenol or oligomycin. TPA-induced cytoskeletal alterations are independent of fibronectin expression and not mimicked, modified, or prevented by calmodulin inhibitors or experimentally elevated levels of calcium and cyclic AMP. Thus the morphological response to TPA involves rapid redistribution of actin and vinculin independent of transcription and translation, fluctuations in the levels of calcium or cyclic AMP, or changes in the organization of microtubules, intermediate filaments, and fibronectin.
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Ballestrem, C., Wehrle-Haller, B., Hinz, B., Imhof, B. A. (2000). Actin-dependent Lamellipodia Formation and Microtubule-dependent Tail Retraction Control-directed Cell Migration. Mol. Biol. Cell 11: 2999-3012 [Abstract] [Full Text]
Krendel, M., Gloushankova, N. A., Bonder, E. M., Feder, H. H., Vasiliev, J. M., Gelfand, I. M. (1999). Myosin-dependent contractile activity of the actin cytoskeleton modulates the spatial organization of cell-cell contacts in cultured epitheliocytes. Proc. Natl. Acad. Sci. USA 96: 9666-9670 [Abstract] [Full Text]
Philpott, D. J., McKay, D. M., Mak, W., Perdue, M. H., Sherman, P. M. (1998). Signal Transduction Pathways Involved in Enterohemorrhagic Escherichia coli-Induced Alterations in T84 Epithelial Permeability. Infect. Immun. 66: 1680-1687 [Abstract] [Full Text]
Greenwood, J. A., Pallero, M. A., Theibert, A. B., Murphy-Ullrich, J. E. (1998). Thrombospondin Signaling of Focal Adhesion Disassembly Requires Activation of Phosphoinositide 3-Kinase. J. Biol. Chem. 273: 1755-1763 [Abstract] [Full Text]
Ribeiro, C. M.�P., Reece, J., Putney Jr., J. W. (1997). Role of the Cytoskeleton in Calcium Signaling in NIH 3T3 Cells. AN INTACT CYTOSKELETON IS REQUIRED FOR AGONIST-INDUCED [Ca2+]i SIGNALING, BUT NOT FOR CAPACITATIVE CALCIUM ENTRY. J. Biol. Chem. 272: 26555-26561 [Abstract] [Full Text]
Hartwig, J. H., Kung, S., Kovacsovics, T., Janmey, P. A., Cantley, L. C., Stossel, T. P., Toker, A. (1996). D3 Phosphoinositides and Outside-in integrin Signaling by Glycoprotein IIb-IIIa Mediate Platelet Actin Assembly and Filopodial Extension Induced by Phorbol 12-Myristate 13-Acetate. J. Biol. Chem. 271: 32986-32993 [Abstract] [Full Text]
Yamakita, Y., Ono, S., Matsumura, F., Yamashiro, S. (1996). Phosphorylation of Human Fascin Inhibits Its Actin Binding and Bundling Activities. J. Biol. Chem. 271: 12632-12638 [Abstract] [Full Text]
Murphy-Ullrich, J., Pallero, M., Boerth, N, Greenwood, J., Lincoln, T., Cornwell, T. (1996). Cyclic GMP-dependent protein kinase is required for thrombospondin and tenascin mediated focal adhesion disassembly. J. Cell Sci. 109: 2499-2508 [Abstract]
Goodnight, J., Mischak, H., Kolch, W., Mushinski, J. F. (1995). Immunocytochemical Localization of Eight Protein Kinase C Isozymes Overexpressed in NIH 3T3 Fibroblasts. J. Biol. Chem. 270: 9991-10001 [Abstract] [Full Text]
Citi, S, Denisenko, N (1995). Phosphorylation of the tight junction protein cingulin and the effects of protein kinase inhibitors and activators in MDCK epithelial cells. J. Cell Sci. 108: 2917-2926 [Abstract]
Turner, C., Pietras, K., Taylor, D., Molloy, C. (1995). Angiotensin II stimulation of rapid paxillin tyrosine phosphorylation correlates with the formation of focal adhesions in rat aortic smooth muscle cells. J. Cell Sci. 108: 333-342 [Abstract]
Cross, D, Vial, C, Maccioni, R. (1993). A tau-like protein interacts with stress fibers and microtubules in human and rodent cultured cell lines. J. Cell Sci. 105: 51-60 [Abstract]
Davis, S, Lu, M., Lo, S., Lin, S, Butler, J., Druker, B., Roberts, T., An, Q, Chen, L. (1991). Presence of an SH2 domain in the actin-binding protein tensin. Science 252: 712-715 [Abstract]