Monoclonal antibody characterization of the C proteins of heterogeneous nuclear ribonucleoprotein complexes in vertebrate cells

doi:10.1083/jcb.99.6.1997

This Article

	Full Text (PDF, 2194K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Choi, Y. D.
	Articles by Dreyfuss, G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Choi, Y. D.
	Articles by Dreyfuss, G.

Social Bookmarking

	What's this?

ARTICLES

Monoclonal antibody characterization of the C proteins of heterogeneous nuclear ribonucleoprotein complexes in vertebrate cells

YD Choi and G Dreyfuss

The C proteins (C1 and C2) are major constituents of the 40S subparticle of heterogeneous nuclear ribonucleoprotein complexes (hnRNPs) (Beyer, A.L., M.E. Christensen, B.W. Walker, and W.M. LeStourgeon, 1977, Cell, 11:127-138) and are two of the most prominent proteins that become cross-linked by ultraviolet light to heterogeneous nuclear RNA (hnRNA) in vivo. Studies are described here on the characterization of the C proteins in vertebrate cells using monoclonal and polyclonal antibodies. Monoclonal antibodies to genuine RNP proteins, including the C proteins, were obtained by immunizing mice with purified complexes of poly(A)+ hnRNA and poly(A)+ mRNA with their contacting proteins in vivo obtained by ultraviolet cross-linking the complexes in intact cells (Dreyfuss, G., Y.D. Choi, and S.A. Adam, 1984, Mol. Cell. Biol., 4:1104-1114). One of the monoclonal antibodies identified the C proteins in widely divergent species ranging from human to lizard. In all species examined, there were two C proteins in the molecular weight range of from 39,000 to 42,000 for C1, and from 40,000 to 45,000 for C2. The two C proteins were found to be highly related to each other; they were recognized by the same monoclonal antibodies and antibodies raised against purified C1 reacted also with C2. In avian, rodent, and human cells the C proteins were phosphorylated and were in contact with hnRNA in vivo. Immunofluorescence microscopy demonstrated that the C proteins are segregated to the nucleus. Within the nucleus the C proteins were not found in nucleoli and were not associated with chromatin as seen in cells in prophase. These findings demonstrate that C proteins with similar characteristics to those in humans are ubiquitous components of hnRNPs in vertebrates.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Winton, M. J., Igaz, L. M., Wong, M. M., Kwong, L. K., Trojanowski, J. Q., Lee, V. M.-Y. (2008). Disturbance of Nuclear and Cytoplasmic TAR DNA-binding Protein (TDP-43) Induces Disease-like Redistribution, Sequestration, and Aggregate Formation. J. Biol. Chem. 283: 13302-13309 [Abstract] [Full Text]
Piazzon, N., Rage, F., Schlotter, F., Moine, H., Branlant, C., Massenet, S. (2008). In Vitro and in Cellulo Evidences for Association of the Survival of Motor Neuron Complex with the Fragile X Mental Retardation Protein. J. Biol. Chem. 283: 5598-5610 [Abstract] [Full Text]
Nelson, P. T., De Planell-Saguer, M., Lamprinaki, S., Kiriakidou, M., Zhang, P., O'Doherty, U., Mourelatos, Z. (2007). A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells. RNA 13: 1787-1792 [Abstract] [Full Text]
TAVANEZ, J. P., CALADO, P., BRAGA, J., LAFARGA, M., CARMO-FONSECA, M. (2005). In vivo aggregation properties of the nuclear poly(A)-binding protein PABPN1. RNA 11: 752-762 [Abstract] [Full Text]
Mili, S., Pinol-Roma, S. (2003). LRP130, a Pentatricopeptide Motif Protein with a Noncanonical RNA-Binding Domain, Is Bound In Vivo to Mitochondrial and Nuclear RNAs. Mol. Cell. Biol. 23: 4972-4982 [Abstract] [Full Text]
Desterro, J. M. P., Keegan, L. P., Lafarga, M., Berciano, M. T., O'Connell, M., Carmo-Fonseca, M. (2003). Dynamic association of RNA-editing enzymes with the nucleolus. J. Cell Sci. 116: 1805-1818 [Abstract] [Full Text]
Kim, J. H., Paek, K. Y., Choi, K., Kim, T.-D., Hahm, B., Kim, K.-T., Jang, S. K. (2003). Heterogeneous Nuclear Ribonucleoprotein C Modulates Translation of c-myc mRNA in a Cell Cycle Phase-Dependent Manner. Mol. Cell. Biol. 23: 708-720 [Abstract] [Full Text]
Calapez, A., Pereira, H. M., Calado, A., Braga, J., Rino, J., Carvalho, C., Tavanez, J. P., Wahle, E., Rosa, A. C., Carmo-Fonseca, M. (2002). The intranuclear mobility of messenger RNA binding proteins is ATP dependent and temperature sensitive. JCB 159: 795-805 [Abstract] [Full Text]
Massenet, S., Pellizzoni, L., Paushkin, S., Mattaj, I. W., Dreyfuss, G. (2002). The SMN Complex Is Associated with snRNPs throughout Their Cytoplasmic Assembly Pathway. Mol. Cell. Biol. 22: 6533-6541 [Abstract] [Full Text]
Gustin, K. E., Sarnow, P. (2002). Inhibition of Nuclear Import and Alteration of Nuclear Pore Complex Composition by Rhinovirus. J. Virol. 76: 8787-8796 [Abstract] [Full Text]
Friesen, W. J., Wyce, A., Paushkin, S., Abel, L., Rappsilber, J., Mann, M., Dreyfuss, G. (2002). A Novel WD Repeat Protein Component of the Methylosome Binds Sm Proteins. J. Biol. Chem. 277: 8243-8247 [Abstract] [Full Text]
Caputi, M., Zahler, A. M. (2001). Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J. Biol. Chem. 276: 43850-43859 [Abstract] [Full Text]
Mili, S., Shu, H. J., Zhao, Y., Pinol-Roma, S. (2001). Distinct RNP Complexes of Shuttling hnRNP Proteins with Pre-mRNA and mRNA: Candidate Intermediates in Formation and Export of mRNA. Mol. Cell. Biol. 21: 7307-7319 [Abstract] [Full Text]
Vautier, D, Chesne, P, Cunha, C, Calado, A, Renard, J., Carmo-Fonseca, M (2001). Transcription-dependent nucleocytoplasmic distribution of hnRNP A1 protein in early mouse embryos. J. Cell Sci. 114: 1521-1531 [Abstract]
Ford, L. P., Suh, J. M., Wright, W. E., Shay, J. W. (2000). Heterogeneous Nuclear Ribonucleoproteins C1 and C2 Associate with the RNA Component of Human Telomerase. Mol. Cell. Biol. 20: 9084-9091 [Abstract] [Full Text]
Wan, L., Dockendorff, T. C., Jongens, T. A., Dreyfuss, G. (2000). Characterization of dFMR1, a Drosophila melanogaster Homolog of the Fragile X Mental Retardation Protein. Mol. Cell. Biol. 20: 8536-8547 [Abstract] [Full Text]
COOVERT, D. D, LE, T. T, MORRIS, G. E, MAN, N. T., KRALEWSKI, M., SENDTNER, M., BURGHES, A. H M (2000). Does the survival motor neuron protein (SMN) interact with Bcl-2?. J. Med. Genet. 37: 536-539 [Full Text]
Brackenridge, S., Proudfoot, N. J. (2000). Recruitment of a Basal Polyadenylation Factor by the Upstream Sequence Element of the Human Lamin B2 Polyadenylation Signal. Mol. Cell. Biol. 20: 2660-2669 [Abstract] [Full Text]
Charroux, B., Pellizzoni, L., Perkinson, R. A., Yong, J., Shevchenko, A., Mann, M., Dreyfuss, G. (2000). Gemin4: A Novel Component of the Smn Complex That Is Found in Both Gems and Nucleoli. JCB 148: 1177-1186 [Abstract] [Full Text]
Wang, W., Furneaux, H., Cheng, H., Caldwell, M. C., Hutter, D., Liu, Y., Holbrook, N., Gorospe, M. (2000). HuR Regulates p21 mRNA Stabilization by UV Light. Mol. Cell. Biol. 20: 760-769 [Abstract] [Full Text]
Melcak, I., Koberna, K., Malínský, J. (2000). Nuclear pre-mRNA Compartmentalization: Trafficking of Released Transcripts to Splicing Factor Reservoirs. Mol. Biol. Cell 11: 497-510 [Abstract] [Full Text]
Charroux, B., Pellizzoni, L., Perkinson, R. A., Shevchenko, A., Mann, M., Dreyfuss, G. (1999). Gemin3: A Novel Dead Box Protein That Interacts with Smn, the Spinal Muscular Atrophy Gene Product, and Is a Component of Gems. JCB 147: 1181-1194 [Abstract] [Full Text]
Piñol-Roma, S. (1999). Association of Nonribosomal Nucleolar Proteins in Ribonucleoprotein Complexes during Interphase and Mitosis. Mol. Biol. Cell 10: 77-90 [Abstract] [Full Text]
Chen, H., Hu, B., Gacad, M. A., Adams, J. S. (1998). Cloning and Expression of a Novel Dominant-Negative-acting Estrogen Response Element-binding Protein in the Heterogeneous Nuclear Ribonucleoprotein Family. J. Biol. Chem. 273: 31352-31357 [Abstract] [Full Text]
Eckmann, C. R., Jantsch, M. F. (1997). Xlrbpa, a Double-stranded RNA-binding Protein Associated with Ribosomes and Heterogeneous Nuclear RNPs. JCB 138: 239-253 [Abstract] [Full Text]
Fung, P.�A., Labrecque, R., Pederson, T. (1997). RNA-dependent phosphorylation of a nuclear RNA binding�protein. Proc. Natl. Acad. Sci. USA 94: 1064-1068 [Abstract] [Full Text]
Chagnovich, D., Fayos, B. E., Cohn, S. L. (1996). Differential Activity of ELAV-like RNA-binding Proteins in Human Neuroblastoma. J. Biol. Chem. 271: 33587-33591 [Abstract] [Full Text]
Waterhouse, N., Kumar, S., Song, Q., Strike, P., Sparrow, L., Dreyfuss, G., Alnemri, E. S., Litwack, G., Lavin, M., Watters, D. (1996). Heteronuclear Ribonucleoproteins C1 and C2, Components of the Spliceosome, Are Specific Targets of Interleukin 1beta -converting Enzyme-like Proteases in Apoptosis. J. Biol. Chem. 271: 29335-29341 [Abstract] [Full Text]
Temsamani, J., Pederson, T. (1996). The C-group Heterogeneous Nuclear Ribonucleoprotein Proteins Bind to the 5' Stem-Loop of the U2 Small Nuclear Ribonucleoprotein Particle. J. Biol. Chem. 271: 24922-24926 [Abstract] [Full Text]
Hozak, P, Sasseville, A., Raymond, Y, Cook, P. (1995). Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells. J. Cell Sci. 108: 635-644 [Abstract]
Ferreira, J, Carmo-Fonseca, M (1995). The biogenesis of the coiled body during early mouse development. Development 121: 601-612 [Abstract]
Buchenau, P, Arndt-Jovin, D., Saumweber, H (1993). In vivo observation of the puff-specific protein no-on transient A (NONA) in nuclei of Drosophila embryos. J. Cell Sci. 106: 189-199 [Abstract]
Pinol-Roma, S, Dreyfuss, G (1991). Transcription-dependent and transcription-independent nuclear transport of hnRNP proteins. Science 253: 312-314 [Abstract]
Takagaki, Y, Manley, J L, MacDonald, C C, Wilusz, J, Shenk, T (1990). A multisubunit factor, CstF, is required for polyadenylation of mammalian pre-mRNAs.. Genes Dev. 4: 2112-2120 [Abstract]
Garcia-Blanco, M A, Jamison, S F, Sharp, P A (1989). Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.. Genes Dev. 3: 1874-1886 [Abstract]
Pinol-Roma, S, Choi, Y D, Matunis, M J, Dreyfuss, G (1988). Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins.. Genes Dev. 2: 215-227 [Abstract]
Choi, Y., Grabowski, P., Sharp, P., Dreyfuss, G (1986). Heterogeneous nuclear ribonucleoproteins: role in RNA splicing. Science 231: 1534-1539 [Abstract]
Sullivan, N.F., Watt, R.A., Delannoy, M.R., Green, C.L., Spector, D.L. (1986). Colocalization of the myc Oncogene Protein and Small Nuclear Ribonucleoprotein Particles. Cold Spring Harb Symp Quant Biol 51: 943-947 [Abstract]
Paushkin, S., Charroux, B., Abel, L., Perkinson, R. A., Pellizzoni, L., Dreyfuss, G. (2000). The Survival Motor Neuron Protein of Schizosacharomyces pombe. CONSERVATION OF SURVIVAL MOTOR NEURON INTERACTION DOMAINS IN DIVERGENT ORGANISMS. J. Biol. Chem. 275: 23841-23846 [Abstract] [Full Text]
Gama-Carvalho, M., Carvalho, M. P., Kehlenbach, A., Valcarcel, J., Carmo-Fonseca, M. (2001). Nucleocytoplasmic Shuttling of Heterodimeric Splicing Factor U2AF. J. Biol. Chem. 276: 13104-13112 [Abstract] [Full Text]
Wan, L., Kim, J.-K., Pollard, V. W., Dreyfuss, G. (2001). Mutational Definition of RNA-binding and Protein-Protein Interaction Domains of Heterogeneous Nuclear RNP C1. J. Biol. Chem. 276: 7681-7688 [Abstract] [Full Text]