In vivo dissection of the chromosome condensation machinery: reversibility of condensation distinguishes contributions of condensin and cohesin

doi:10.1083/jcb.200109056

Published online 25 February 2002. doi:10.1083/jcb.200109056
The Rockefeller University Press, 0021-9525 $8.00
The Journal of Cell Biology

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Article

In vivo dissection of the chromosome condensation machinery

: reversibility of condensation distinguishes contributions of condensin and cohesin

Brigitte D. Lavoie¹, Eileen Hogan² and Douglas Koshland²

¹ Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON M5S 1A8, Canada
² Department of Embryology, Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, MD 21210

Address correspondence to Brigitte D. Lavoie, Department of Medical Genetics and Microbiology, University of Toronto, Medical Sciences Building, Room 4278, Toronto, ON M5S 1A8, Canada. Tel.: (416) 978-6123. Fax: (416) 978-6885. E-mail: brigitte.lavoie{at}utoronto.ca

The machinery mediating chromosome condensation is poorly understood.To begin to dissect the in vivo function(s) of individual components,we monitored mitotic chromosome structure in mutants of condensin,cohesin, histone H3, and topoisomerase II (topo II). In buddingyeast, both condensation establishment and maintenance requireall of the condensin subunits, but not topo II activity or phospho-histoneH3. Structural maintenance of chromosome (SMC) protein 2, aswell as each of the three non-SMC proteins (Ycg1p, Ycs4p, andBrn1p), was required for chromatin binding of the condensincomplex in vivo. Using reversible condensin alleles, we showthat chromosome condensation does not involve an irreversiblemodification of condensin or chromosomes. Finally, we providethe first evidence of a mechanistic link between condensin andcohesin function. A model discussing the functional interplaybetween cohesin and condensin is presented.

Key Words: sister chromatid cohesion; mitotic chromosome structure; SMC proteins; chromosome dynamics; MCD1/SCC1

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