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Published online 30 May 2006. doi:10.1083/jcb.200511125
 The Rockefeller University Press, 0021-9525 $8.00
The Journal of Cell Biology
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ARTICLE

An intimate collaboration between peroxisomes and lipid bodies



Derk Binns1, Tom Januszewski2, Yue Chen3, Justin Hill1, Vladislav S. Markin4, Yingming Zhao3, Christopher Gilpin2, Kent D. Chapman5, Richard G.W. Anderson2, and Joel M. Goodman1

1 Department of Pharmacology, 2 Department of Cell Biology, 3 Department of Biochemistry, and 4 Department of Anesthesiology, University of Texas Southwestern Medical School, Dallas, TX 75390
5 Department of Biological Sciences, University of North Texas, Denton, TX 76203

Correspondence to Joel M. Goodman: Joel.Goodman{at}UTSouthwestern.edu

Although peroxisomes oxidize lipids, the metabolism of lipid bodies and peroxisomes is thought to be largely uncoupled from one another. In this study, using oleic acid–cultured Saccharomyces cerevisiae as a model system, we provide evidence that lipid bodies and peroxisomes have a close physiological relationship. Peroxisomes adhere stably to lipid bodies, and they can even extend processes into lipid body cores. Biochemical experiments and proteomic analysis of the purified lipid bodies suggest that these processes are limited to enzymes of fatty acid ß oxidation. Peroxisomes that are unable to oxidize fatty acids promote novel structures within lipid bodies ("gnarls"), which may be organized arrays of accumulated free fatty acids. However, gnarls are suppressed, and fatty acids are not accumulated in the absence of peroxisomal membranes. Our results suggest that the extensive physical contact between peroxisomes and lipid bodies promotes the coupling of lipolysis within lipid bodies with peroxisomal fatty acid oxidation.

Abbreviations used in this paper: PNS, postnuclear supernatant.


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