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doi:10.1083/jcb.200904118
The Journal of Cell Biology
The Rockefeller University Press, 0021-9525 $30.00
© Hayashi et al.
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Drosophila glypicans regulate the germline stem cell niche



Yoshiki Hayashi1,2, Satoru Kobayashi2, and Hiroshi Nakato1

1 Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
2 Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki 444-8787, Japan

Correspondence to Hiroshi Nakato: nakat003{at}umn.edu

Stem cells are maintained in vivo by short-range signaling systems in specialized microenvironments called niches, but the molecular mechanisms controlling the physical space of the stem cell niche are poorly understood. In this study, we report that heparan sulfate (HS) proteoglycans (HSPGs) are essential regulators of the germline stem cell (GSC) niches in the Drosophila melanogaster gonads. GSCs were lost in both male and female gonads of mutants deficient for HS biosynthesis. dally, a Drosophila glypican, is expressed in the female GSC niche cells and is responsible for maintaining the GSC niche. Ectopic expression of dally in the ovary expanded the niche area, showing that dally is required for restriction of the GSC niche space. Interestingly, the other glypican, dally-like, plays a major role in regulating male GSC niche maintenance. We propose that HSPGs define the physical space of the niche by serving as trans coreceptors, mediating short-range signaling by secreted factors.

Abbreviations used in this paper: Bam, bag of marbles; BMP, bone morphogenetic protein; Dlp, Dally-like; Dpp, decapentaplegic; E-Cad, epithelial cadherin; FasIII, fasciclin III; Gbb, Glass-bottom boat; GSC, germline stem cell; HS, heparan sulfate; HSPG, HS proteoglycan; Hts, Hu-li tai shao; JAK/STAT, Janus kinase/signal transducers and activators of transcription; PGC, primordial germ cell; sfl, sulfateless; ttv, tout-velu; UAS, upstream activation sequence; Upd, Unpaired; Vas, Vasa.

© 2009 Hayashi et al.
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