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© The Rockefeller University Press,
0021-9525/1998//147 $5.00
The Journal of Cell Biology, Volume 143, Number 1,
, 1998 147-157
Regular Articles |
Motile Properties of Vimentin Intermediate Filament Networks in Living Cells
The motile properties of intermediate filament (IF) networks have been studied in living cells expressing vimentin tagged with green fluorescent protein (GFP-vimentin). In interphase and mitotic cells, GFP-vimentin is incorporated into the endogenous IF network, and accurately reports the behavior of IF. Time-lapse observations of interphase arrays of vimentin fibrils demonstrate that they are constantly changing their configurations in the absence of alterations in cell shape. Intersecting points of vimentin fibrils, or foci, frequently move towards or away from each other, indicating that the fibrils can lengthen or shorten. Fluorescence recovery after photobleaching shows that bleach zones across fibrils rapidly recover their fluorescence. During this recovery, bleached zones frequently move, indicating translocation of fibrils. Intriguingly, neighboring fibrils within a cell can exhibit different rates and directions of movement, and they often appear to extend or elongate into the peripheral regions of the cytoplasm. In these same regions, short filamentous structures are also seen actively translocating. All of these motile properties require energy, and the majority appear to be mediated by interactions of IF with microtubules and microfilaments.
Key Words: intermediate filaments • vimentin • microtubules • microfilaments • green fluorescent protein
Abbreviations used in this paper: GFP, green fluorescent protein; IF, intermediate filaments; MT, microtubules; MF, microfilaments; FRAP, fluorescence recovery after photobleaching; t1/2, recovery half-time.
INTERMEDIATE filaments (IF)1 are major cytoskeletal components of mammalian cells that appear to be relatively stable structures because of their insolubility in vitro and the lack of biochemical evidence for the existence of significant pools of soluble subunits in vivo (Steinert et al., 1976; Zackroff and Goldman, 1979; Soellner et al., 1985). However, there is increasing evidence that intermediate filaments are dynamic structures in vivo. For example, they form juxtanuclear aggregates in response to microinjection of IF antibodies and heat shock (Klymkowsky, 1981; Collier et al., 1993). In mitosis, partial or complete disassembly of IF networks takes place, followed by their reassembly in daughter cells (Franke et al., 1982; Rosevear et al., 1990). Furthermore, the state of IF polymerization is regulated by subunit exchange in vivo as suggested by the findings that microinjected vimentin and keratin, as well as newly synthesized IF proteins, become incorporated into preexisting IF networks (Vikstrom et al., 1989; Albers and Fuch, 1989; Ngai et al., 1990; Miller et al., 1993). The most direct evidence supporting an equilibrium between unpolymerized IF subunits and polymerized IF in vivo has been obtained from fluorescence recovery after photobleaching (FRAP) analyses (Vikstrom et al., 1992; Okabe et al., 1993).
Interactions of IF with IF-associated proteins (IFAPs), microtubules (MT), and microfilaments (MF) are thought to regulate IF organization in vivo (Chou et al., 1997). Structural evidence for IF–MT interactions comes from the observation of close parallel arrays of IF and MT, as well as the reorganization of IF to form perinuclear caps in response to MT depolymerization (Goldman and Knipe, 1973). Furthermore, MAP-2 (Hirokawa et al., 1988) and the IFAP plectin (Svitkina et al., 1996) have been shown to form cross-bridges between IF and MT. Evidence supporting IF–MF interactions comes from ultrastructural observations demonstrating close associations between IF and MF (Schliwa and van Blerkom, 1981), and from the finding that colcemid-induced movement of vimentin IF into perinuclear caps is inhibited by cytochalasin D (Hollenbeck et al., 1989). Candidates for cross-bridging elements between IF and MF include plectin and dystonin/ BPAG1n (Brown et al., 1995; McLean et al., 1996; Yang et al., 1996).
Very little is known about the motile properties of IF due primarily to the difficulties inherent in visualizing IF in vivo. Recently, many of these difficulties have been alleviated by expression of proteins tagged with the green fluorescent protein (GFP; Cubitt et al., 1995). Direct observations of GFP-tagged cytoskeletal proteins in vivo have provided insights into the dynamic properties of MT, MAP-4, actin, and IF (Olson et al., 1995; Ludin and Matus, 1998; Fischer et al., 1998; Ho et al., 1998).
In this study we describe in detail the motile properties of vimentin IF using GFP fused with vimentin (GFP-vimentin). After transfection with GFP-vimentin, time-lapse and FRAP analyses show that IF networks constantly change their configurations in vivo. GFP-vimentin fibrils are motile; they can translocate, alter their shapes, and apparently extend and shorten over relatively short time intervals. Time-lapse imaging of GFP-vimentin at the cell periphery reveals unexpected movement of short filamentous structures (termed squiggles). The effects of nocodazole and cytochalasin B indicate that many, but not all of these motile properties of IF are dependent on MT and/or MF.
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Cell Cultures and Transient Transfection
Baby hamster kidney (BHK-21) fibroblasts were grown in DME supplemented with 10% tryptose phosphate broth (Difco Laboratories Inc., Detroit, MI), 10% calf serum, and antibiotics (100 U/ml penicillin and streptomycin). SW13 vim– cells (a gift of Dr. Robert Evans, University of Colorado) were grown in DME with 10% FCS and antibiotics. Bovine pulmonary arterial endothelial cells (CPAE; American Type Culture Collection, Rockville, MD) were grown in DME with 2 mM glutamine, 10% FCS, and antibiotics. HeLa and PtK2 cells were grown in MEM with 10% FCS, 0.1 mM nonessential amino acid solution (GIBCO-BRL, Gaithersburg, MD), and antibiotics.
For transfection, LipofectamineTM (GIBCO-BRL) was used according to the manufacturer's protocol. Cells grown on coverslips were incubated with a mixture of 1 µg of CsCl-purified DNA and 6 µl of LipofectamineTM in serum-free medium for 3 h, after which the mixture was replaced with standard culture medium.
Preparation of IF-enriched Cytoskeletons and Immunoprecipitation
IF-enriched cytoskeletal preparations were made from confluent BHK-21 cell cultures (Zackroff and Goldman, 1979), and the final pellet was solubilized and sonicated in 10 mM Tris-HCl, pH 6.8, 5 mM EDTA, 2% SDS, 10% glycerol, and CompleteTM protease inhibitors (Boehringer Mannheim Corp., Indianapolis, IN). The resulting solution was diluted 1:20 in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP-40, 5 mM EDTA, and CompleteTM protease inhibitors, and was used for immunoprecipitation experiments.
Immunoprecipitation of GFP-vimentin was carried out with Protein A-sepharose (Sigma Chemical Co., St. Louis, MO) and 9E10, a mouse monoclonal anti-human c-myc antibody (Evan et al., 1985; American Type Culture Collection) as described elsewhere (Chou et al., 1990). The immunoprecipitates were separated by SDS-PAGE (Laemmli, 1970). Immunoblotting was carried out according to Towbin et al. (1979). The primary antibodies used were 9E10 and a mouse monoclonal anti-vimentin (Sigma Chemical Co.). The secondary antibody used was peroxidase-conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories Inc., West Grove, PA).
Indirect Immunofluorescence
Cells grown on coverslips were fixed and processed for indirect immunofluorescence as described by Yang et al. (1985). The primary antibodies used were 9E10, a rabbit polyclonal anti-BHK IF (Yang et al., 1985), a rabbit polyclonal anti-bovine tongue keratin antibody (Jones et al., 1988) and a mouse monoclonal anti-β-tubulin (Amersham Life Science, Inc., Arlington Heights, IL). The secondary antibodies used were fluorescein-conjugated goat anti–mouse IgG and Lissamine rhodamine (LRSC)-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, Inc.).
Microscopy
For live cell studies, transfected cells were trypsinized 48 h after transfection, and were plated onto coverslips to achieve 70% confluence in standard culture medium containing 10 mM Hepes, pH 7.0. The coverslips were placed on small glass feet (to prevent compression) on slides, sealed with a mixture of Vaseline, beeswax, and lanolin (1:1:1), and maintained at 37°C with an air stream stage incubator (Model ASI 400; NEVTEK, Burnsville, VA). In some cases, these coverslips were treated with nocodazole (Sigma Chemical Co.) at final concentrations of either 100 or 600 nM. In other cases, cytochalasin B (Sigma Chemical Co.) was added at final concentrations of either 2 or 20 µM. For metabolic inhibition studies, final concentrations of both 50 mM 2-deoxy-D-glucose (Sigma Chemical Co.) and 0.05% sodium azide (Sigma Chemical Co.) were added. For cell-cycle studies, chromosomes were stained with 2 µg/ml of Hoechst 33258 (Molecular Probes Inc., Eugene, OR).
Time-lapse observations were made with an LSM 410 confocal microscope (Carl Zeiss, Inc., Thornwood, NY) equipped with a 100x, 1.4 NA oil immersion objective. Phase-contrast images of cells were taken before and after time-lapse observations to ensure that no significant change in cell shape or position occurred during the periods of observation. Digitized images were collected every 30 s for 30–40 min using a fluorescein filter set (excitation at 488 nm and emission at 515–560 nm). To test the stability of the focal plane in the confocal microscope, we made time-lapse observations of MT patterns for 30 min in fixed BHK cells after staining with anti-β-tubulin. No obvious change in focus was detected during this time interval. This minimized problems due to focal plane drift during the periods of observation.
In some cases, indirect immunofluorescence observations were made using a Zeiss Axiophot with a CCD camera (Photometrics, Tucson, AZ) controlled by Metamorph imaging software (Universal Imaging Corp., West Chester, PA).
Fluorescence Recovery After Photobleaching (FRAP)
FRAP was carried out with the LSM 410 confocal microscope (Carl Zeiss, Inc., Thornwood, NY). Phase-contrast images of cells were taken before and immediately after FRAP to ensure that there were no significant changes in cell shape or position. Bar-shaped regions were bleached using the line-scan function at 488 nm (100% power, 1% attenuation), and recovery of fluorescence was monitored (15–30% power, 10% attenuation) using the time-series function at 2-min intervals for up to 1 h.
Image Analyses
Position, length, and intensity measurements were made on digitized confocal images using Metamorph image analysis software. Pixel values were converted to distance using confocal calibration bars. Analyses of the dynamic properties of GFP-vimentin fibrils were restricted to cells that showed no obvious shape changes for 30–40 min. If the average displacement of a cell's margins was more than 2 µm in 40 min (0.05 µm/min) as indicated by phase contrast, it was excluded from the analyses. Rates of translocation of vimentin fibrils were determined by monitoring distance vs. time. If vimentin fibrils or foci moved at least 0.25 µm in 5 min (0.05 µm/min), the data were used to calculate the rates of movements.
FRAP half-times (t1/2) were calculated by monitoring the gray-scale pixel values (intensity measurements) across the bleached vimentin fibrils using the line-scan function of Metamorph at each time point after adjustment for sample fading during time-lapse observations (see Vikstrom et al., 1992). All data are reported as mean ± SD.
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30% of the BHK-21 cells 72 h after transfection. The majority of these cells displayed typical cytoplasmic IF networks (Fig. 1, A and B), indistinguishable from those seen by immunofluorescence (Yang et al., 1985). GFP-vimentin colocalized with endogenous vimentin as demonstrated by double-label immunofluorescence (see Fig. 6, B, and E–F). GFP-vimentin also formed typical IF networks in CPAE endothelial cells (see Fig. 5). In HeLa (data not shown) and PtK2 cells that have two IF networks, GFP-vimentin was incorporated only into the vimentin and not the keratin IF network (Fig. 1, C and D). GFP-vimentin does not appear to alter the properties of interphase IF networks as indicated by observations such as their reorganization into a perinuclear cap after the disassembly of MT (Fig. 1, E and F; see Yang et al., 1992).
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4–5% of the cells expressed GFP-vimentin. However, only 1% of these displayed typical IF patterns, while the remainder displayed only aggregates of vimentin (data not shown; see Ho et al., 1998). This low level of expression of typical IF networks may be related to the leaky expression of vimentin that we detected in 1% of SW13 vim– cells. Furthermore, when these vim– cells were cotransfected with wild-type vimentin (Chou et al., 1996) and GFP-vimentin, 20% of the cells displayed typical IF networks (data not shown). These results indicate that GFP-vimentin is capable of assembling into IF only in the presence of a wild-type background.
GFP-vimentin with the c-myc tag was tested at the biochemical level for its ability to incorporate into endogenous IF networks in BHK-21 cells. 72 h after transfection, IF-enriched cytoskeletons were prepared from cultures with 30% transfected cells. SDS-PAGE analyses indicated the presence of vimentin, other IF-associated proteins, and a minor 82-kD band corresponding to the molecular weight of GFP-vimentin (Fig. 3 A). Immunoblot analyses of these preparations showed that the 82-kD band reacted with c-myc antibody (Fig. 3 B). These cytoskeletal preparations were also solubilized, immunoprecipitated with c-myc antibody, and analyzed by SDS-PAGE and immunoblotting. The results showed a single band of 82 kD recognized by c-myc antibody (Fig. 3 C), and both the 82-kD fusion protein and 55-kD untagged vimentin with vimentin antibody (Fig. 3 D). These results provide further support that GFP-vimentin is incorporated into the endogenous IF network.
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The fluorescent IF networks were characterized by extensive arrays of fibrils. In some cases, fibrils appeared to be interconnected at diffusely fluorescent focal points (foci). The majority of the vimentin fibrils and foci displayed movements during the periods of observation. These foci were useful indicators of motility as they frequently moved towards or away from each other (Fig. 4, A–C). The average rate of translocation of the foci and therefore changes in the length of the interconnecting fibrils was 0.42 ± 0.16 µm/min (n = 21). In no case did we observe a change in the direction of the movement of foci. However, this might be due to the limited time intervals of observation.
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FRAP Analyses
In our earlier FRAP studies, after microinjecting rhodamine-labeled vimentin, we showed that there is an equilibrium between unpolymerized vimentin IF subunits and polymerized vimentin IF in vivo. The main drawback of our previous study was our inability to estimate the average recovery half-time (t1/2) due to relatively rapid background photobleaching (Vikstrom et al., 1992). A major advantage of using GFP fusion proteins is that fluorescence intensity does not decrease significantly during image acquisition over relatively long periods (Cubitt et al., 1995). Since our observations suggested that many fibrils were motile (Figs. 4, 5), FRAP analyses also provided another means of monitoring IF translocation during recovery, especially in regions of the cytoplasm containing parallel arrays of fibrils without a significant number of interconnecting foci.
To ensure that photobleaching did not damage vimentin IF in vivo, BHK cells expressing GFP-vimentin-myc were fixed 1 min after bleaching, and were processed for double indirect immunofluorescence (Fig. 6). The continuous vimentin-staining patterns across the bleach zones indicated that photobleaching did not disrupt the integrity of the vimentin fibrils.
Time-lapse observations were made at 2-min intervals for 60 min after photobleaching. In some cytoplasmic regions, bleach zones were relatively stationary during recovery (Fig. 7, A–D). FRAP analyses of such stationary fibrils indicated that the fluorescence intensities along bleached vimentin fibrils increased with time, and that no preferential recovery from either side could be detected (Fig. 8). The average time for the bleach zones to completely recover their fluorescence was 23 ± 12 min with an average t1/2 of 5 ± 3 min (n = 25).
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Motile Properties of Short Vimentin Fibrils
In addition to the typical networks of interconnecting fibrils seen in transfected cells, short filamentous structures termed vimentin squiggles were frequently visible. These were most apparent in the thin peripheral regions of BHK-21 cells (Fig. 9, A–F). Vimentin squiggles were also seen in fixed untransfected BHK-21 and PtK2 cells (data not shown).
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Nocodazole Treatment Affects the Motile Properties of IF
Low concentrations of nocodazole (e.g., 100 nM) are known to interfere with MT dynamics without significantly affecting the number or distribution of MT (Liao et al., 1995; Vasquez et al., 1997). The overall distributions of MT and IF in BHK-21 cells treated with 100 nM nocodazole for 30–60 min were similar to those of untreated cells as determined by fixation and double indirect immunofluorescence (data not shown). To determine whether the motile properties of GFP-vimentin fibrils in vivo were dependent on MT dynamics, we carried out time-lapse observations and FRAP analyses after adding 100 nM nocodazole for 30 min. Vimentin-rich foci continued to move relative to each other, and the fibrils interconnecting them appeared to shorten or lengthen at an average rate of 0.18 ± 0.06 µm/min (n = 16), representing a decrease of 58% compared with control cells. Fibrils also continued to change their shapes under these experimental conditions. FRAP analyses indicated that fibrils recovered their fluorescence more slowly at a t1/2 of 8 ± 5 min (n = 16). During recovery, some bleach zones moved at an average rate of 0.14 ± 0.09 µm/min (n = 22), a 43% decrease relative to control cells. At the cell periphery, 93% (296/318) of the squiggles translocated at an average rate of 3.4 ± 1.6 µm/ min (n = 16), ranging from 1.5 to 9.9 µm/min, which was similar to controls. We also studied fibril extension at the edges of endothelial cells in this concentration of nocodazole and found the average rate was 1.6 ± 0.7 µm/min (n = 20), which was also similar to controls.
When BHK cells were treated with 600 nM nocodazole for 5 h, MT depolymerized (data not shown), and the majority of vimentin IF reorganized into perinuclear caps (Fig. 1, E–F). Under these conditions, some vimentin fibrils remained extended towards the cell periphery, and squiggles could be found near the cell surfaces; however, it was difficult to locate foci (Fig. 10 A; see Yang et al., 1992). Time-lapse observations showed that the extended fibrils continued to change their shapes (Fig. 10, B and C), and FRAP analyses showed that they recovered their fluorescence more slowly at a t1/2 of 8 ± 3 min (n = 12). During recovery, some bleach zones moved at a rate of 0.1 ± 0.07 µm/min (n = 7), representing a 60% decrease compared with controls. The majority of vimentin squiggles (381/400 = 95%) did not move during the periods of observation. However, 5% of the squiggles continued to move at a rate of 3 ± 1.9 µm/min (n = 19; range 0.4–8.9 µm/min), which was similar to controls.
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When transfected cells were exposed to 20 µM cytochalasin B for 30 min, BHK-21 cell margins retracted, and the overall morphology became arborized (Fig. 10 D; see Goldman and Knipe, 1973). FRAP analyses showed complete but slower recovery with a t1/2 of 11 ± 5 min (n = 12). Some bleach zones moved at 0.09 ± 0.04 µm/min (n = 7), a 60% decrease compared with controls, and squiggles continued to translocate (92/105 = 88%) at an average rate of 3.3 ± 2.4 µm/min (n = 17; range 0.4–6.4 µm/min; Fig. 10, D–F), again similar to controls. Shape changes of fibrils and squiggles were seen at both concentrations of cytochalasin B (data not shown).
The Motile Properties of IF Require Metabolic Energy
To determine whether ATP is required for the motile properties of IF, we made time-lapse observations of GFP-vimentin IF networks after treating BHK-21 cells with 50 mM 2-deoxy-D-glucose and 0.05% sodium azide for 30 min (Donaldson et al., 1990). None of the movements described above were detected over time periods of up to 40 min after 30 min of pretreatment with these inhibitors (Fig. 10, G–H). These results demonstrate that the motile properties of vimentin IF require metabolic energy.
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Discussion |
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The Motile Properties of Vimentin IF
Time-lapse observations of interphase cells expressing GFP-vimentin showed that fibrils exhibit numerous types of motility. For example, fibrils frequently appeared and disappeared in the same focal plane in the thin sheets of cytoplasm at the cell periphery. It is possible that these IF fibrils were moving in or out of the plane of focus. However, it is also possible that these observations represent local assembly or disassembly of IF (also see Prahlad et al., 1998). We also observed apparent fibril extension in the peripheral regions of cells. However, at the present time we cannot determine whether this extension of fibrils is due to active growth and elongation of fibrils or to the movement of preexisting fibrils due to sliding with respect to other cytoskeletal components such as MT and/or MF.
Vimentin fibrils also appeared to be capable of lengthening and shortening as evidenced by the movement of interconnected foci either towards or away from each other. Movements of parallel arrays (not interconnected by foci) of vimentin fibrils were also observed due to the movements of bleach zones. Interestingly, both of these types of IF motility are sensitive to nocodazole and cytochalasin B, indicating a dependence on both MT and MF network integrity. It should be noted that there is evidence that the movement of IF towards the nucleus is MF dependent (Hollenbeck et al., 1989), and that their movement towards the cell surface is MT-dependent. The latter dependency appears to be related to a kinesin motor activity (Gyoeva and Gelfand, 1991; Prahlad et al., 1998).
Short filamentous structures with two free ends, vimentin squiggles (also see Osborn, 1980; Prahlad et al., 1998), exhibited rapid movements, primarily towards the edge of cells at speeds up to 11 µm/min. Squiggles continued to translocate at the same rates as controls in the presence of cytochalasin B. However, only 5% of the squiggles moved at the same rates as controls in the absence of MT. These observations suggest that translocation of the vast majority of squiggles is dependent on MT, most likely through MT-associated motor molecules such as kinesin. The observation that a small number of squiggles continue to translocate in the absence of MT is intriguing, and suggests that the motility of a subpopulation may be dependent on either MF and their associated proteins or unknown factors. In support of the former possibility, IF have been shown to interact with MF, and proteins have been identified that can form cross-bridges between these two cytoskeletal systems (Brown et al., 1995; McLean et al., 1996; Yang et al., 1996).
It is also interesting to compare the rates of movement of bleach zones and photoactivated fluorescent marks along MT and MF with the rates obtained for IF in this study. For MT, analyses of mitotic and interphase MT bleach zones have revealed rates of 0.1–0.7 µm/min (Gorbsky and Borisy, 1989; Mitchison, 1989; Waterman-Storer and Salmon, 1997). Bleach zones made across microspikes containing fluorescent MF moved at 0.79 ± 0.31 µm/min (Wang, 1985), and in stress fibers the rates were 0.29 ± 0.1 µm/min (McKenna and Wang, 1986). Therefore, the rate of translocation of bleach zones on vimentin fibrils is similar to the rates recorded for MT and MF, irrespective of the differences in the mechanisms responsible for these movements (i.e., treadmilling for MT and MF). Intriguingly, adjacent vimentin fibrils move either towards or away from the cell center at different speeds while other fibrils in neighboring cytoplasmic regions remain stationary. These observations are different from the unidirectional movements of bleached zones toward the nucleus thus far reported for interphase MT and MF (McKenna and Wang, 1986; Waterman-Storer and Salmon, 1997).
Vimentin fibrils frequently changed their shapes as evidenced by bending or straightening movements within short time intervals. These movements continued in the presence of nocodazole and cytochalasin B, suggesting that they represent intrinsic motile properties of IF independent of MT and MF. Interestingly, metabolic inhibitors completely arrested these shape changes, demonstrating that these movements require energy.
Use of GFP-vimentin To Monitor Subunit Exchange In Vivo
Using GFP-vimentin in FRAP analyses has permitted us to calculate accurately a recovery half-time (t1/2) of 5 ± 3 min in BHK-21 cells. Similar analyses of interphase MT have indicated a t1/2 of 4.5 ± 1.2 min in PtK1 cells, and 3.3 ± 1.4 min in BSC cells (Saxton et al., 1984). In the case of actin in stress fibers, a t1/2 of 5–10 min has been reported (Kreis et al., 1982). Therefore, the recovery half-times for vimentin IF are very similar to those made for the other major cytoskeletal components. It is also interesting that the fluorescence recovery appeared to slow down relative to controls in the presence of MT and MF inhibitors. Therefore, the exchange mechanisms involved in the steady-state turnover of vimentin may depend to some unknown extent on either MT or MF. It is conceivable that in the case of MT, the movement of exchangeable vimentin subunits may be related to the kinesin-based motility of vimentin IF (Gyoeva and Gelfand, 1991) and the recently discovered protofilamentous aggregates of IF protein found in spreading fibroblasts (Prahlad et al., 1998).
Type III IF networks containing vimentin exhibit a remarkable diversity of motile properties that require energy. The majority of these properties appear to reflect interactions with MT, MF, and their associated proteins (see Chou et al., 1997). Only the shape changes exhibited by IF appear to be independent of the other cytoskeletal systems. The mechanism underlying the changes in shape may be related to posttranslational modifications such as phosphorylation/dephosphorylation, which are thought to be involved in regulating the assembly and disassembly of IF (Eriksson et al., 1992; Inagaki et al., 1996). In this regard, it has been hypothesized that the hierarchical structure of IF produces a thermodynamically unstable seam that spirals along their length (Stewart, 1993). It is likely that this seam contains the most readily exchangeable subunits within IF. Perhaps the differential removal or addition of subunits in the region of this seam relative to more rigid regions of the IF wall could account for the changes in the shape of vimentin fibrils in vivo.
In summary, GFP-vimentin fibrils can translocate, appear, or disappear within short time intervals, alter their shapes, and apparently alter their lengths. The significance of these motile properties of IF remains unknown. However, the surprisingly complex and continuous movements of intermediate filaments raise interesting new questions regarding their functions and their interactions with other cytoskeletal systems.
Submitted: 3 June 1998
Revised: 26 August 1998
We greatly appreciate the excellent technical assistance of Ms. Satya Khuon and Ms. Laura Davis for assistance in typing this manuscript.
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