Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text (PDF, 42K) Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                            What's this?

© The Rockefeller University Press, 0021-9525/1999//205 $5.00
The Journal of Cell Biology, Volume 147, Number 1, , 1999 205

© 1999 The Rockefeller University Press

The cDNA sequence coding for SFT (stimulator of Fe transport) published in the article by Gutierrez et al. contains several errors. Corrected sequence data are available from GenBank under accession number AF020761. These changes indicate that the open reading frame originally predicted to encode the SFT protein is incorrect; several potential but smaller open reading frames are present in the corrected nucleotide sequence. The functional activity associated with SFT cDNA, i.e., stimulation of Fe transport in transfected cells, has been reconfirmed as described in this article and resequencing of the pGL2-SFT construct used for these functional studies confirms the sequence changes except for an AT conversion at position 725 and a nucleotide deletion at position 883. Recent BLAST analysis of the nonredundant GenBank/EMBL/DDBJ/PDB sequences indicates a 46–base pair stretch of identity between nucleotides 530–573 of human SFT and nucleotides 414–457 of human ubiquitin-conjugating enzyme E2131 (UBE2D1, accession number X78140) and a search of human EST sequences identifies at least two GenBank entries, accession numbers AA188127 and AA146924, that overlap with both UBE2D1 and SFT. Our laboratory is currently determining the true open reading frame responsible for SFT function and evaluating the potential relationship between UBE2D1 and SFT. We wish to apologize for any additional work that the sequencing errors may have caused other investigators.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Gehrke, S. G., Riedel, H.-D., Herrmann, T., Hadaschik, B., Bents, K., Veltkamp, C., Stremmel, W. (2003). UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood 101: 3288-3293 [Abstract] [Full Text]  

This Article Full Text (PDF, 42K) Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                            What's this?