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© The Rockefeller University Press,
0021-9525/1999//869 $5.00
The Journal of Cell Biology, Volume 147, Number 4,
, 1999 869-878
Original Article |
Skeletal Myogenic Progenitors Originating from Embryonic Dorsal Aorta Coexpress Endothelial and Myogenic Markers and Contribute to Postnatal Muscle Growth and Regeneration
cossu{at}axrma.uniroma1.it
Skeletal muscle in vertebrates is derived from somites, epithelial structures of the paraxial mesoderm, yet many unrelated reports describe the occasional appearance of myogenic cells from tissues of nonsomite origin, suggesting either transdifferentiation or the persistence of a multipotent progenitor. Here, we show that clonable skeletal myogenic cells are present in the embryonic dorsal aorta of mouse embryos. This finding is based on a detailed clonal analysis of different tissue anlagen at various developmental stages. In vitro, these myogenic cells show the same morphology as satellite cells derived from adult skeletal muscle, and express a number of myogenic and endothelial markers. Surprisingly, the latter are also expressed by adult satellite cells. Furthermore, it is possible to clone myogenic cells from limbs of mutant c-Met–/– embryos, which lack appendicular muscles, but have a normal vascular system. Upon transplantation, aorta-derived myogenic cells participate in postnatal muscle growth and regeneration, and fuse with resident satellite cells.
The potential of the vascular system to generate skeletal muscle cells may explain observations of nonsomite skeletal myogenesis and raises the possibility that a subset of satellite cells may derive from the vascular system.
Key Words: myogenesis • satellite cells • endothelial cells • multipotent progenitors • vascular–endothelial cadherin
© 1999 The Rockefeller University Press
IT is generally accepted that all skeletal muscle cells of the vertebrate body derive from the dorsal domain of somites, epithelial structures that form in a cranio–caudal sequence from the paraxial mesoderm, flanking the axial neural tube on both sides ( Christ and Ordhal 1995). This evidence largely derives from classic chick–quail transplantation experiments, and has been confirmed by in vitro explant culture in both birds and mammals (reviewed in Cossu et al. 1996a).
Skeletal myogenesis begins soon after onset of somitogenesis and continues throughout development and postnatal growth. Adult muscle retains proliferative potential due to the presence of quiescent satellite cells. Cells in the somites are first instructed to become myogenic by signals emanating from neighboring tissues, such as notochord, neural tube, and dorsal ectoderm ( Cossu et al. 1996a). Once committed, a subset of dorsal epithelial somite cells will begin migrating beneath the dermomyotome to form the myotome, the first skeletal muscle structure, initially composed of terminally differentiated postmitotic, mononucleate cells ( Denetclaw et al. 1997; Kahane et al. 1998). Cells of the dermomyotome, exposed to the same signals, enter other mesodermal lineages, such as dermis or endothelium, or remain undifferentiated as progenitors of successive generations of myogenic cells that will later give rise to primary and secondary fibers, as well as to satellite cells.
Most experiments addressing the origin of skeletal muscle have analyzed only the embryonic stages of development. It has been assumed that skeletal muscle cells that form during fetal and postnatal life share the same origin, although experimental proof for this is lacking.
However, much evidence has accumulated for in vivo or in vitro myogenic differentiation of cells of nonsomite origin during late fetal and postnatal development (reviewed in Cossu 1997), when satellite cells are considered to be the only remaining myogenic progenitors ( Bischoff 1994). Whether or not satellite cells originate exclusively from somites also remains an open question.
We recently reported that some myogenic cells that participate in skeletal muscle regeneration originate in the bone marrow and are delivered to the muscle through the blood circulation ( Ferrari et al. 1998). This prompted us to define the embryological origin of these myogenic cells, as well as of satellite cells, but lack of specific markers has hampered this work. Lineage studies for skeletal muscle are also complicated by the multinucleate nature of the differentiated tissue. However, it is possible to clone mammalian satellite cells that, in the mouse, show a typical round-shaped morphology during proliferation, clearly different from spindle-shaped myoblasts ( Cossu et al. 1987). Upon serum withdrawal, these cells will elongate and then fuse into multinucleate contracting myotubes.
By extending this clonal analysis to various early embryonic structures, we now report that the highest yield in myogenic clones with a morphology typical of satellite cells and expressing a number of myogenic and endothelial markers was obtained from dorsal aorta, a tissue not previously known to give rise to skeletal myogenic cells. These results have implications for current interpretations of fetal and postnatal myogenesis.
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Materials and Methods |
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Cell and Organ Cultures
Embryos were dissected and embryonic structures were isolated as described ( Cossu et al. 1996b). The tissues were then either separated into a single cell suspension by gentle pipetting or cultured as explants ( Cusella-De Angelis et al. 1994) for periods ranging from 3–7 d. At the end of the culture period, tissues were either stained for β-galactosidase activity, immunofluorescence, or used for reverse transcriptase (RT)1 -PCR. Cultures were grown as described ( Cossu et al. 1996b). Northern Blot analysis and RT-PCR were performed as described ( Ferrari et al. 1997). Oligos for Pax3 amplification were 5'-TGT GGA ATA GAC GTG GGC TGG TA-3' and 5'-AGG AGG CGG ATC TAG AAA GGA AG-3'; for MyoD: 5'- CAC TAC AGT GGC GAC TCA GAC GCG-3', nt 730–753, 5'-CCT GGA CTC GCG CAC CGC CTC ACT-3', nt 873–850.
Immunocytochemistry
The antibodies used against MyoD, myosin heavy chains, and c-Met have been described ( Bader et al. 1982; Tajbakhsh et al. 1994; Koishi et al. 1995; Maina et al. 1996). The antibodies against M-cadherin and MNF were donated by A. Starzyski-Powitz (Humangenetik fur Biologen, Goethe-Universitat, Frankfurt, Germany) and R. Bussel-Duby (University of Texas SW Medical Center, Dallas, TX), respectively ( Irintchev et al. 1994; Garry et al. 1997). The antibodies against vascular-endothelial (VE) -cadherin, CD34, and PECAM were donated by E. Dejana and C. Gherlanda (Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy). Antibodies against VEGR2, 
M-integrin (MAC-1), β3-integrin, and von Willebrand (Santa Cruz and Pharmingen) were donated by A. Stoppacciaro (Dipartimento di Medicinal Sperimentale e Patologia, Universita ‘La Sapienza,’ Rome, Italy). Immunocytochemistry on tissue sections and cultured cells was carried out as described ( Tajbakhsh et al. 1994).
Transplantation Studies
Genetically labeled cells were injected into the regenerating Tibialis anterior (TA) of SCID/bg mice as described ( Ferrari et al. 1998). Fetal limbs were isolated from E16–17 wild-type (wt) embryos and, after removal of the skin, were transplanted subcutaneously into newborn (P1–2) MLC3F-nlacZ mice ( Lagrand et al. 1997). Alternatively, freshly dissected dorsal aortas from E9 MLC3F-nlacZ embryos were transplanted into the TA of newborn (P4–5) SCID/bg mice. At different periods after transplantation, the mice were killed, the transplanted and the contralateral TA muscles or the transplanted fetal limb were recovered, and stained for β-galactosidase activity or cryostat-sectioned and processed for immunofluorescence.
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We next investigated whether the aorta would maintain the potential of generating clones of satellite cell-like cells during later developmental stages. Fig. 3 B shows that this potential declines during development, but at E17 is still approximately one third of the maximal value obtained at E9.5. Postnatal or adult aorta, as well as smaller vessels dissected from limb muscle, did not give rise to satellite cell-like clones, either directly or after explant culture (not shown). Forelimbs from the same stage were used as positive controls.
Endothelial Markers Are Expressed in Myogenic Cells from Aorta and in Satellite Cells
These novel results led us to compare the phenotype of these clonable satellite cell-like cells with that of adult satellite cells and fetal myoblasts. Table and Fig. 4 show that most known myogenic markers (MyoD, Myf-5, desmin, MNF, c-Met, and M-cadherin) were expressed by clones of dorsal aorta, as well as by postnatal muscle satellite cells and fetal myoblasts. Unexpectedly, vascular-endothelial markers, such as VE-cadherin, VEGF-R2, M-integrin, β3 integrin, P-selectin, smooth -actin, and PECAM were also expressed by the first two cell types, while fetal myoblasts did not express VE-cadherin, P-selectin and β3 integrin (all clones were negative for von Willebrand factor). Fig. 5 shows clones of aorta-derived myogenic cells ( Fig. 5 A) and of adult satellite cells ( Fig. 5 B) that all coexpress VE-cadherin on the surface and MyoD in the nucleus. Clones from dorsal aorta also expressed CD34, but only during the first two days in vitro. Muscle fibroblasts (used as controls) were negative for all of these markers. Thus, clones from the dorsal aorta were indistinguishable from clones of satellite cells in terms of differential expression of any of 15 different markers analyzed. Expression of VE-cadherin was confirmed by Northern blot analysis in satellite cells ( Fig. 6). It was unexpected, and is notable that satellite cells derived from adult muscle express a number of endothelial markers.
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50% of those obtained from heterozygous or wt siblings). This demonstrates the presence of clonogenic satellite cell-like cells that are not derived from somitic myoblasts. The clones obtained from mutant embryos were notably smaller (averaging 4 to 6 cells) than those derived from the heterozygous siblings (averaging 20 to 60 cells), and expressed high levels of MyoD ( Fig. 7F and Fig. G). With time in culture, some of these clones disappeared and occasionally fragmented chromatin was observed, but the surviving clones differentiated into myosin-positive mono- or binucleate muscle cells.
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50%) of those obtained from wt embryos. Similar results were also obtained using limb buds of Splotch embryos as a source of myogenic cells (not shown). Splotch mice are defective in the Pax-3 gene and their phenotype overlaps with the Met null in terms of lack of migration of somite-derived precursors in the limbs ( Bober et al. 1994; Goulding et al. 1994).
In Vivo Myogenic Potential of Cells from Dorsal Aorta
We tested the potential of transplanted aorta-derived satellite cell-like cells to participate in in vivo perinatal growth and regeneration of skeletal muscle, in three different sets of experiments.
In the first experiment, a cell suspension from cultured explants of E9.5 embryonic aorta of MLC3F-nLacZ transgenic mice, in which transgene expression is restricted to heart and skeletal muscle ( Kelly et al. 1995), was mixed with a tenfold excess of satellite cells from wt P10 mice. Part of this suspension was grown in culture. Fig. 8 A shows a myotube containing one β-galactosidase positive (β-gal+) nucleus which, because of the genetic label, must be derived from the dorsal aorta explant. The rest of the mixed cell suspension was injected into a regenerating TA muscle of SCID/bg mice, where they gave rise to small clusters of several β-gal+ nuclei within regenerating fibers, surrounded by a laminin-positive basal membrane ( Fig. 8B and Fig. C). Thus, cells derived from the dorsal aorta can fuse with satellite cells in vitro and participate in skeletal muscle regeneration in vivo. All these results show that myogenic cells derived from the aorta cannot be distinguished from bona fide satellite cells.
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In explant cultures, we found that dorsal aorta did not contain differentiated muscle cells, which typically appear in similar cultures of somitic or limb tissues. However, when dorsal aortas were first explant-cultured for a week, and then dissociated to single cell suspensions and cloned under conditions that favor expansion of adult satellite cells, many myogenic clones appeared, undistinguishable from bona fide satellite cells. The fact that such clones could not be obtained directly from the aorta suggests a requirement for growth factors provided by neighboring cells during the incubation as organ culture, and absent in our culture medium.
The myogenic clones express all the early myogenic markers considered, including M-cadherin, MNF, and desmin. Virtually 100% of these cells express MyoD, while only a minority of them express Myf-5. Although the majority of postnatal satellite cells, upon clonal expansion in vitro or activation in vivo, express both MyoD and Myf5 ( Cornelison and Wold 1997; Cooper et al. 1999), in the MyoD knockout mouse regeneration is impaired via an effect on the satellite cell population ( Megeney et al. 1996).
The unexpected coexpression of a number of endothelial and myogenic markers in cells derived from the dorsal aorta suggests that these myogenic cells may be derived from true endothelial cells or from a common precursor. It is interesting to note that endothelial cells appear to be the only cell type capable of generating immortal clones in early postimplantation mouse embryos ( Hatzopoulos et al. 1998) and that circulating endothelial progenitor cells can be isolated from adult human blood ( Asahara et al. 1997). These endothelial progenitors typically express CD34. Aorta-derived myogenic clones only express CD34 at the beginning of clonal expansion, suggesting early loss of this antigen upon entry in the myogenic pathway. Other endothelial markers, such as VE-cadherin, are lost upon terminal differentiation, but are coexpressed with MyoD throughout clonal expansion.
To our surprise, endothelial markers were also coexpressed with MyoD in bona fide satellite cells, a puzzling observation for cells presumed to be of somitic origin. The problem of the embryonic origin of satellite cells has never been directly approached, except in a study that was inconclusive due to technical difficulties in identifying quail nuclei in chick–quail chimeras at the ultrastructural level ( Armand et al. 1983). It is interesting to note that, unlike postnatal satellite cells, fetal myoblasts were found not to express VE-cadherin, β3-integrin, or P-selectin, in keeping with the possibility that several phenotypically distinct populations of myogenic cells may appear sequentially during muscle histogenesis ( Cossu and Molinaro 1987).
Our data suggest that, differently from embryonic and fetal myoblasts derived from somites, satellite cells may derive, at least in part, from progenitors in the dorsal aorta. Coexpression of endothelial and myogenic markers supports this hypothesis. In normal development, endothelial cells first migrate into the limb bud from the lateral edge of newly formed somites and are soon followed by myogenic cells ( Solursh et al. 1987). In contrast, only endothelial cells (but not myogenic precursors) enter the limb bud of embryos mutant for c-Met or Pax3. The embryos do not survive until the stage when satellite cells appear in vivo. However, explant cultures from limb buds of these embryos yielded a significant number of satellite cell-like clones (50% of wt), supporting the idea that they are the progeny of endothelial cells, rather than myoblasts. Interestingly, all the cells of these clones express MyoD, do not grow to more than four to eight cells, and, for the most part, eventually die, but the few surviving undergo terminal muscle differentiation. Met signaling in response to SF/HGF thus appears to be required for the clonal growth of these newly identified myogenic cells.
Myogenic Progenitors from the Dorsal Aorta Contribute to Postnatal Muscle Growth and Regeneration
Expression of myogenic markers in vitro suggests a potential for in vivo myogenic differentiation. Our results indicate that these myogenic cells do participate in both postnatal muscle growth and regeneration. When directly injected into the regenerating TA of an immune-deficient mouse, genetically labeled nuclei of cells from the dorsal aorta are incorporated into newly formed muscle fibers, much as bona fide satellite cells. Indeed, skin fibroblasts also are incorporated into regenerating fibers when similarly injected, although at an extremely low frequency ( Gibson et al. 1995). Thus, while regenerating muscle must be a rich source of signals recruiting competent cells to myogenesis, it is also possible that within a population of skin fibroblasts, there may be a small fraction of still multipotent progenitors ( Bianco and Cossu 1999) and it is only this fraction that is capable of differentiating in vivo.
When a fetal limb is transplanted under the skin of a transgenic MLC3F-nlacZ newborn mouse, it is vascularized by the host. These limbs contain β-gal+ nuclei, usually clustered in the area of the vessel in-growth, suggesting that they were associated with it, rather than deriving from neighboring host muscle. Finally, when an embryonic dorsal aorta is transplanted into the growing TA of a newborn immune-deficient mouse, it gives rise to many β-gal+ nuclei clustered in the area of the transplant. This does not happen if the aorta is grown in vitro, indicating that signals from the surrounding developing skeletal muscle recruit some of the aorta cells (endothelium and/or pericytes) to myogenesis. Remarkably, several dispersed β-gal+ nuclei are also present in the contralateral untreated TA, indicating that these myogenic cells must have reached this site through the circulation, much as happens for the adult progenitors in bone marrow ( Ferrari et al. 1998). Possible contamination from adjacent somitic tissue is ruled out because when somites are similarly transplanted, they give rise to differentiated muscle cells only in the area of transplant.
Transdifferentiation or Multipotentiality?
Our data may simply represent one example of transdifferentiation leading to the formation of skeletal muscle cells, as reported for the esophagus ( Patapoutian et al. 1995), the neural tube ( Tajbakhsh et al. 1994), the kidney ( Mayer and Leinwand 1997), or mesenchymal cells from bone marrow ( Grigoriadis et al. 1988) and dermis ( Salvatori et al. 1995). We propose a different explanation: that, at least in this case and in the case of mesenchymal cells, multipotent progenitors may be present in the endothelium (or closely associated cells). When invading developing muscle anlagen, these progenitors will be subject to a muscle field, and thus will adopt a satellite cell fate. When the vessels develop inside a different tissue, these cells may adopt the specific fate of that tissue, and contribute to its histogenesis. The only tissue in which these progenitors remain demonstrable may be the bone marrow, and this would explain our recent observation that cells from the bone marrow can contribute new myogenic cells to regenerating skeletal muscle ( Ferrari et al. 1998). Multipotent mesenchymal cells, capable of producing osteoblasts, chondroblasts, adipocytes, and even skeletal muscle, have long been known to be present in the bone marrow ( Caplan 1991; Prockop 1997). We do not know whether the cells we describe in embryonic vessels represent the progenitors of multipotent mesenchymal cells or a separate lineage with at least part of the same developmental potential. Preliminary observations suggest that clones of dorsal aorta can give rise to osteoblast-like cells in the presence of BMP-2. Indeed, multipotentiality is preserved, even in adult muscle satellite cells, as shown by the fact that BMP2 can switch them to an osteogenic fate ( Katagiri et al. 1994).
In vivo work will clarify whether the contribution of aorta-derived myogenic cells is quantitatively relevant during fetal and/or postnatal growth and regeneration of skeletal muscle.
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Acknowledgments |
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This work was supported by grants from Telethon, European Community, Fondazione Cenci Bolognetti, Agenzia Spaziale Italiana, and Ministero dell'Università e della Ricerca Scientifica e Technologica.
Submitted: 6 July 1999
Revised: 29 September 1999
Accepted: 4 October 1999
1.used in this paper: β-gal+, β-galactosidase positive; MetD, Met receptor unable to transduce the HGF signal; RT, reverse transcriptase; TA, Tibialis anterior; VE, vascular–endothelial; wt, wild-type
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