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© The Rockefeller University Press,
0021-9525/1999//1 $5.00
The Journal of Cell Biology, Volume 147, Number 6,
, 1999 1-2
In Brief |
© 1999 The Rockefeller University Press
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A Potential Flippase in Trafficking |
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 Top A Potential Flippase in... Balancing Forces in MeiosisLinking Actin and MicrotubulesPKA Anchors and Lets... |
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Chen et al. identify a drs2 mutation as being synthetically lethal with a deletion in ADP-ribosylation factor 1 (ARF1). ARF1 is one of two redundant ARFs. Both recruit two coat proteins: COPI for Golgi to ER transport, and clathrin, possibly for TGN to endosome transport. Drs2p appears to function in the clathrin pathway, as a drs2 deletion interacts only with mutations in clathrin and a protein from clathrin-coated pits, and the deletion strain accumulates excess Golgi membranes and fewer clathrin-coated vesicles. Drs2p localizes to the TGN.
Drs2p is most similar to the bovine P-type ATPase II, a possible aminophospholipid translocase (flippase). ATPase II was originally thought to be responsible for flipping phosphatidylserine from the external leaflet of the lipid bilayer to prevent recognition of the cell by the apoptotic apparatus. Drs2p seemed to fit this bill for yeast, as the mutant apparently failed to flip a lipid derivative across the plasma membrane, and an earlier study localized Drs2p by fractionation to the plasma membrane.
But in a more recent study, the drs2 mutant did not show differences in lipid transport across the plasma membrane. The new localization results of Chen et al. suggest that any possible Drs2p flippase activity would be found in the TGN, not at the plasma membrane.
The ATPase activity of Drs2p is necessary for its trafficking function, but Todd Graham says he must now confirm that the enzyme is a flippase, and see if Drs2p activity has any effect in vitro on the tubulation or ARF recruitment of purified Golgi membranes.
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Balancing Forces in Meiosis |
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TopA Potential Flippase in...Balancing Forces in MeiosisLinking Actin and MicrotubulesPKA Anchors and Lets... |
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The linkages, or chiasmata, are the products of recombination that normally hold chromosome pairs together in the first meiotic division. A fly's fourth chromosomes are small and packed with poorly recombining heterochromatin, so they must do without chiasmata. They resort instead to a weak heterochromatin-based affinity that usually breaks apart soon after spindle formation, and well before the extended metaphase arrest is released in the oocyte. Up to 10% of fly X chromosomes also fail to recombine and follow a similar pathway of achiasmate segregation, and many errors in human chromosome segregation involve chromosomes that show no signs of prior recombination.
The first of the balanced forces is directed polewards. It may be generated by microtubule flux or a motor at the kinetochore; in these experiments it is eliminated by a mutation in the tub67C gene, which encodes a divergent 
tubulin. Chromatin in the mutant is no longer stretched along the spindle during metaphase arrest, and centromeres are not distributed to the polewards ends of the chromatin mass. The result is an increase in achiasmate non-disjunction.
These phenotypes are reversed by reducing the dosage of the Nod kinesin, the source of the opposing force. Nod attaches to DNA, and has been postulated to provide a force pushing chromosomes back into the spindle. Some Nod is needed to keep chromosomes from flying out of the spindle, but too much Nod pushes the achiasmate chromosomes together even after the heterochromatin linkage has dissolved, thus confounding any heterochromatin-directed segregations.
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Linking Actin and Microtubules |
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TopA Potential Flippase in...Balancing Forces in MeiosisLinking Actin and MicrotubulesPKA Anchors and Lets... |
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MACF is a hybrid protein: it has an actin-binding amino terminus resembling some of the plakins (which link actin and intermediate filaments), whereas its central stem and part of the carboxy-terminal tail resemble proteins from the spectrin superfamily. The third, novel, component is a unique microtubule-binding domain at the carboxyl terminus.
MACF is a homologue of the Drosophila protein Kakapo, which was described in three papers in the November 30, 1998 issue of this journal. An actin filament–microtubule linking function would explain the varied structural and neuronal defects in kakapo mutants. In other cells, an actin filament–microtubule linker could impart rigidity, anchor and position the spindle, and transfer neuronal vesicles from microtubules to actin.
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PKA Anchors and Lets Go |
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TopA Potential Flippase in...Balancing Forces in MeiosisLinking Actin and MicrotubulesPKA Anchors and Lets... |
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Collas et al. find that PKA activity is needed for maintenance of chromatin condensation. As AKAP95 anchors the regulatory subunits of PKA, the catalytic subunits may phosphorylate chromatin substrates in a burst as the catalytic subunits are released, before they cycle back onto the chromatin, possibly in a preformed complex with the regulatory subunits and AKAP95.
AKAP95, but not PKA, is needed for the establishment of chromatin condensation. AKAP95 antibodies prevent the recruitment of the condensin component Eg7 to chromatin, and AKAP95 and Eg7 coprecipitate. This suggests that, like other AKAPs, AKAP95 may anchor more than PKA.
Letting Go of MAP Kinase
The cyclic AMP (cAMP)-activated PKA is known to influence the MAP kinase pathway, for example at the level of Ras action. Blanco-Aparicio et al. (page 1129) and Saxena et al. (Saxena, M., S. Williams, K. Taskén and T. Mustelin. 1999. Nat. Cell Biol. 1:305–311) now show that PKA can also activate MAP kinases by disabling their association with protein tyrosine phosphatases that contain a MAP kinase interaction motif (KIM).
Blanco-Aparicio et al. find that, both in vitro and in vivo, PKA phosphorylates a serine within the KIM of the brain-specific phosphatase PTP-SL. This causes PTP-SL to release the MAP kinases from its grasp, thus allowing the MAP kinases to activate and enter the nucleus. Saxena et al. define a similar mechanism used by the hematopoietic tyrosine phosphatase HePTP, and demonstrate that it is sufficient to induce downstream events such as c-fos transcription.
By William A. Wells, 1095 Market St. #516, San Francisco, CA 94103. E-mail: wells@biotext.com
Related Articles
J. Cell Biol. 1999 147: 1129-1136.
-Tubulin 67C Gene Specifically Impair Achiasmate Segregation in Drosophila melanogaster
J. Cell Biol. 1999 147: 1137-1144.
J. Cell Biol. 1999 147: 1167-1180.
J. Cell Biol. 1999 147: 1223-1236.
J. Cell Biol. 1999 147: 1275-1286.
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