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© The Rockefeller University Press, 0021-9525/2000//239 $5.00
The Journal of Cell Biology, Volume 148, Number 2, , 2000 239-246

Brief Report

Nuclear Eukaryotic Initiation Factor 4e (Eif4e) Colocalizes with Splicing Factors in Speckles



Josée Dostiea, Flavio Lejbkowicza, and Nahum Sonenberga

a Department of Biochemistry and Cancer Centre, McGill University, Montréal, Québec H3G 1Y6, Canada
Department of Biochemistry and Cancer Centre, McGill University, 3655 Drummond Street, Montréal, Québec H3G 1Y6, Canada.(514) 398-1287(514) 398-7274

nsonen{at}med.mcgill.ca

The eukaryotic initiation factor 4E (eIF4E) plays a pivotal role in the control of protein synthesis. eIF4E binds to the mRNA 5' cap structure, m7GpppN (where N is any nucleotide) and promotes ribosome binding to the mRNA. It was previously shown that a fraction of eIF4E localizes to the nucleus (Lejbkowicz, F., C. Goyer, A. Darveau, S. Neron, R. Lemieux, and N. Sonenberg. 1992. Proc. Natl. Acad. Sci. USA. 89:9612–9616). Here, we show that the nuclear eIF4E is present throughout the nucleoplasm, but is concentrated in speckled regions. Double label immunofluorescence confocal microscopy shows that eIF4E colocalizes with Sm and U1snRNP. We also demonstrate that eIF4E is specifically released from the speckles by the cap analogue m7GpppG in a cell permeabilization assay. However, eIF4E is not released from the speckles by RNase A treatment, suggesting that retention of eIF4E in the speckles is not RNA-mediated. 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB) treatment of cells causes the condensation of eIF4E nuclear speckles. In addition, overexpression of the dual specificity kinase, Clk/Sty, but not of the catalytically inactive form, results in the dispersion of eIF4E nuclear speckles.

Key Words: nuclear proteins • peptide initiation factor • RNA caps • RNA splicing • cap-binding protein

© 2000 The Rockefeller University Press


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
In eukaryotes, all RNA polymerase II transcripts acquire cotranscriptionally the 5'-terminal structure, m7G(5')ppp-(5')N termed cap ( Salditt-Georgieff et al. 1980). The cap consists of a 7-methyl-guanosine residue, linked via a 5'–5' triphosphate bridge to the first transcribed nucleotide ( Shatkin 1976). The cap is involved in numerous aspects of mRNA metabolism, including RNA stability ( Furuichi et al. 1977; Shimotohno et al. 1977; Murthy et al. 1991), 3' end mRNA processing ( Hart et al. 1985), pre-mRNA splicing ( Konarska et al. 1984; Edery and Sonenberg 1985), nuclear export ( Hamm and Mattaj 1990; Jarmolowski et al. 1994), and translation initiation ( Shatkin 1985). Mammalian eukaryotic initiation factor 4E (eIF4E) is a 24-kD cap-binding protein ( Sonenberg et al. 1978, Sonenberg et al. 1979) that can be purified as a monomer or as a subunit in a complex called eIF4F. eIF4F is a heterotrimeric complex composed of eIF4E, eIF4A, and eIF4G. eIF4A is an ATP-dependent RNA helicase and eIF4G is a modular scaffolding protein that binds to both eIF4E and eIF4A and other proteins ( Imataka and Sonenberg 1997; for review see Gingras et al. 1999). eIF4E plays an important role in the control of gene expression. It is the limiting factor for translation initiation ( Duncan et al. 1987) and its overexpression in NIH3T3 cells causes malignant transformation ( Lazaris-Karatzas et al. 1990). Conversely, in vivo depletion of eIF4E by antisense RNA partially reverses the transformed phenotype of ras-overexpressing cells ( Rinker-Schaeffer et al. 1993).

Nuclear cap-binding proteins that are likely to mediate the various nuclear functions of the cap have been detected and some identified ( Patzelt et al. 1983; Rozen and Sonenberg 1987; Izaurralde et al. 1992). For example, the nuclear cap-binding complex (CBC), composed of CBP20 and CBP80, is involved in mRNA splicing ( Izaurralde et al. 1994; Lewis et al. 1996). CBC also stimulates nucleocytoplasmic export of UsnRNAs ( Izaurralde et al. 1995). A study on the Balbiani ring mRNA export of Chironomus tentans demonstrates that CBC binds cotranscriptionally to the cap and accompanies the ribonucleoprotein particle during nuclear export ( Visa et al. 1996). Additionally, CBC stimulates mRNA 3' end processing ( Flaherty et al. 1997).

Cellular fractionation and immunofluorescence analysis demonstrated that a sizeable fraction (12–33%) of total eIF4E is localized to the nucleus of mammalian cells ( Lejbkowicz et al. 1992). Electron microscope studies showed that eIF4E is also present in the nucleus of Saccharomyces cerevisiae ( Lang et al. 1994). These results raise the possibility that eIF4E may also play a nuclear role in mRNA metabolism, such as splicing or transport. Many, but not all, splicing factors are concentrated in subnuclear structures termed "speckles". The speckles (20–50 speckles per nucleus) are irregular shaped bodies. Although the precise function of the speckles remains controversial, there is evidence that the speckles are sites of posttranscriptional splicing ( Xing et al. 1993, Xing et al. 1995) and of splicing component storage and/or assembly ( Puvion and Puvion-Dutilleul 1996; Spector 1996). Here, we show that the nuclear fraction of eIF4E colocalizes with splicing factors in the speckles. We demonstrate that the nuclear distribution of eIF4E is sensitive to RNA polymerase II transcription inhibitors and the availability of cap structures, but not to RNase treatment. Similar to serine/arginine-rich (SR) splicing factors, the localization of eIF4E is regulated by the dual specificity kinase, Clk/Sty.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Plasmids and Antibodies
Plasmids encoding myc-Clk/Sty wt and myc-Clk/StyK190R ( Colwill et al. 1996) and rabbit anti-myc antibody (A-14) were kindly provided by J.C. Bell (University of Ottawa, Ottawa, Canada). Human anti-Sm sera and human anti-U1snRNP sera were provided by the Center for Disease Control (Atlanta, GA). mAb SC35 was a kind gift from X.D. Fu and T. Maniatis (Harvard University, Cambridge, MA). 10C6 is an anti-mouse eIF4E mAb ( Lejbkowicz et al. 1992). Texas red- and fluorescein-conjugated secondary antibodies were purchased from Molecular Probes, Inc.

Immunofluorescence Assay
CV-1 monkey kidney cells and HeLa cells were plated at 2 x 104 per chamber on Lab-Tek chamber slides (Nunc) and grown to subconfluence in DME supplemented with 10% FBS. Cells were fixed for 1 h with 4% formaldehyde in PBS and permeabilized for 1 h with 4% formaldehyde/0.2% Tween 20 in PBS at room temperature (RT). Cells were briefly rehydrated with 0.2% Tween 20 in PBS before blocking overnight in a solution containing 50% FBS, 6% skim milk, 3% BSA, 0.2% Tween 20, and 0.02% sodium azide. Cells were incubated with primary antibodies for 2 h at RT or overnight at 4°C, and washed extensively with 0.2% Tween 20/PBS before and after incubation with secondary antibodies for 30 min to 1 h at RT. Cells were mounted in 30% glycerol in PBS and analyzed by confocal microscopy. For incubation of HeLa cells with drugs, cycloheximide was added at a final concentration of 20 µM and 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB) was used at 100 µM.

Cell Permeabilization Assay
The assay was done as described previously, except for a few modifications ( Adam et al. 1990). In brief, HeLa cells were plated at low density on coverslips, grown in DME/10%FBS for at least 24 h, and the media was changed 2–4 h before the experiment. Coverslips were briefly rinsed in transport buffer (20 mM Hepes/KOH, pH 7.3, 110 mM potassium acetate, 2 mM sodium acetate, 5 mM magnesium acetate, 1 mM EGTA, 2 mM ditriothreitol, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin) and incubated for 4 min at RT in transport buffer containing 40 µg/ml digitonin. Coverslips were gently rinsed and inverted on a parafilm sheet over a drop of transport buffer containing 2 µg/µl BSA and 50 mM m7GpppG or GpppG. The reaction was done at 30°C for 25 min. Cells were rinsed, fixed in transport buffer containing 3% formaldehyde, and processed for immunofluorescence as described above. This experiment was performed three times with the same results.

RNase Digestion
RNase treatment was performed as previously described ( Spector et al. 1991). In brief, cells (CV-1) were fixed in methanol for 2 min at –20°C, rinsed in PBS, and incubated with RNase A (100 µg/ml) for 2 h at RT. Cells were washed several times and processed for immunofluorescence as described.

Transient Transfections
HeLa cells were plated at low density on 60-mm dishes in DME/10% FBS. At 50% confluency, cells were transfected with 10 µg of plasmid DNA by the calcium phosphate transfection method ( Graham and Eb 1973). Cells were fixed and processed for immunofluorescence 24 h after transfection.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
Nuclear eIF4E Colocalizes with Splicing Factors
An mAb (10C6) that recognizes eIF4E in the nucleus ( Lejbkowicz et al. 1992) was used for immunostaining of HeLa cells. The immunofluorescence shows that nuclear eIF4E is excluded from the nucleoli and concentrated in speckles that are characteristic of those observed to contain splicing factors ( Fig. 1). To determine whether eIF4E colocalizes with splicing factors, antibodies against three different components of the splicing machinery were used: anti-SC35 (an SR protein; Fu and Maniatis 1990); anti-Sm; and anti-U1snRNP. To ascertain that incubation with the three antibodies results in the characteristic nuclear pattern reported earlier, double label immunofluorescence was performed ( Lerner et al. 1981; Fu and Maniatis 1990). Staining with anti-SC35 ( Fig. 2 A, a and d), anti-Sm ( Fig. 2 A, b), and anti-U1snRNP ( Fig. 2 A, e) shows a typical speckled pattern. In addition, SC35 colocalizes with Sm ( Fig. 2 A, c) and U1snRNP ( Fig. 2 A, f), as visualized in the merged pictures and as previously described ( Fu and Maniatis 1990).


Figure 1
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  		Figure 1 Nuclear eIF4E localizes to speckles. The localization of endogenous eIF4E was determined by indirect immunofluorescence with a monoclonal anti-mouse eIF4E antibody, 10C6, and Texas red-conjugated secondary antibody. HeLa cells were fixed and processed for immunofluorescence as described in Materials and Methods. Images were taken from a 63x objective of a Zeiss LSM 410 microscope and analyzed with a Kontron IBAS confocal imaging system. Bar, 10 µm.

 

Figure 2
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  		Figure 2 eIF4E colocalizes with Sm and U1snRNP antigens to speckled regions in the nucleus. A, a–c, SC35 colocalizes with Sm. The localization of endogenous SC35 was determined by indirect immunofluorescence with a monoclonal anti-mouse SC35 antibody and Texas red-conjugated anti-mouse IgG (a and c), and that of Sm with a human anti-Sm sera and fluorescein-conjugated anti-human IgG (b and c). d–f, SC35 colocalizes with U1snRNP. Same as in a–c, except that a human anti-U1snRNP sera was used in e and f. Bar, 5 µm. B, a–c, eIF4E colocalizes with Sm. The localization of eIF4E was determined by indirect immunofluorescence with the monoclonal anti-mouse eIF4E antibody 10C6 and Texas red-conjugated anti-mouse IgG (a and c), and that of Sm with a human anti-Sm sera and fluorescein-conjugated anti-human IgG (b and c). d–f, Same as in a–c, except that a human anti-U1snRNP sera was used in e and f. Bar, 10 µm. In c and f (in both A and B), the colocalization appears yellow. CV-1 monkey kidney cells were prepared as described in Materials and Methods. Images were taken from a 63x objective of a BioRad MRC-600 confocal imaging system mounted on a Nikon Diaphot-TMD microscope as previously described ( Lejbkowicz et al. 1992).

 
To examine whether eIF4E colocalizes with Sm and U1snRNP, cells were incubated with anti-eIF4E ( Fig. 2 B, a and d), anti-Sm ( Fig. 2 B, b), or anti-U1snRNP ( Fig. 2 B, e). The merged images show that eIF4E colocalizes with Sm and U1snRNP ( Fig. 2 B, c and f). It is thought that the speckles represent storage and/or assembly sites for many splicing and transcription components ( Bregman et al. 1995; Spector 1996), as well as splicing sites ( Xing et al. 1993, Xing et al. 1995). Consequently, eIF4E might be involved in nuclear mRNA processing.

The Nuclear Distribution of eIF4E Is Sensitive to RNA Polymerase II Transcription
Inhibition of RNA polymerase II transcription by the nucleoside DRB was shown to cause the rounding up of splicing factor speckles ( Spector et al. 1993). To determine whether the localization of nuclear eIF4E is also sensitive to RNA pol II transcription inhibition, HeLa cells were treated with DRB alone or in the presence of cycloheximide to inhibit protein synthesis. Addition of DRB in the presence or absence of cycloheximide resulted in the rounding up of eIF4E ( Fig. 3c and Fig. d), SC35 ( Fig. 3g and Fig. h), and Sm ( Fig. 3k and Fig. l) speckles. Similar results were also obtained when cells were treated with {alpha}-amanitin (data not shown). Incubation with cycloheximide alone had no effect on the distribution of either protein ( Fig. 3b, Fig. f, and Fig. g). These results show that similar to splicing components, the nuclear distribution of eIF4E is sensitive to RNA pol II transcription and is independent of translation.


Figure 3
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  		Figure 3 Effect of transcription inhibition on the localization of eIF4E. HeLa cells were incubated with buffer alone (a, e, and i), cycloheximide (b, f, and j), DRB (c, g, and k), or in the presence of cycloheximide and DRB (d, h, and l) for 3 h. Cells were fixed and the localization of eIF4E, SC35, and Sm was determined by indirect immunofluorescence with the anti-eIF4E mAb 10C6 (top), anti-mouse SC35 mAb (middle), and with a human anti-Sm antisera (bottom). Primary antibodies were detected with Texas red-conjugated secondary antibodies and analyzed with a Kontron IBAS confocal imaging system. Images were taken from a 63x objective of a Zeiss LSM 410 microscope. Bar, 10 µm.

 
eIF4E Is Released from the Nucleus by the Monomethylated Cap Analogue, but Not by RNase Treatment
Because eIF4E mediates the mRNA cap structure function during translation initiation, it was of interest to determine the effect of cap analogues on eIF4E localization. HeLa cells were permeabilized with digitonin, rinsed to remove soluble proteins, and incubated with m7GpppG or GpppG. Following treatment, cells were rinsed to wash away proteins that were released from the nucleus upon treatment. Incubation with buffer alone did not alter the speckled pattern of eIF4E or SC35 ( Fig. 4 A, left; compare with Fig. 2 A). However, incubation of cells with m7GpppG specifically released eIF4E from the nucleus ( Fig. 4 A, middle), whereas GpppG had no significant effect ( Fig. 4 A, right). The signal intensity for SC35 was not significantly changed by m7GpppG or GpppG. These results might suggest that the nuclear eIF4E is associated with the cap structure of mRNAs in the speckles. Alternatively, it is possible that eIF4E in the speckles is not RNA-bound, but is released from the speckles as a consequence of its interaction with the vast excess of exogenous cap analogue in the nucleoplasm, which competes for the binding of eIF4E with speckled elements.


Figure 4
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  		Figure 4 Nuclear eIF4E is released from the speckles by the monomethylated cap structure, but not by RNase treatment. A, HeLa cells were permeabilized with digitonin and incubated with buffer alone (left), m7GpppG (middle), or GpppG (right) for 25 min. The cells were rinsed and fixed as described in Materials and Methods. The localization of eIF4E (top) and SC35 (bottom) was determined by indirect immunofluorescence with the anti-eIF4E mAb 10C6 and monoclonal anti-SC35, respectively. Primary antibodies were detected with Texas red-conjugated mouse secondary antibodies and analyzed with a Kontron IBAS confocal imaging system. Images were taken from a 63x objective of a Zeiss LSM 410 microscope. Bar, 10 µm. B, CV-1 cells were fixed with methanol and incubated with PBS (a–c) or 100 µg/ml RNase A. The localization of eIF4E (a and d), SC35 (b and e), and Sm (c and f) was determined by indirect immunofluorescence with the anti-eIF4E mAb 10C6, monoclonal anti-mouse SC35, and human anti-Sm sera, respectively. Primary antibodies were detected with Texas red-conjugated mouse secondary antibodies. Images were taken from a 63x objective of a BioRad MRC-600 confocal imaging system mounted on a Nikon Diaphot-TMD microscope as previously described ( Lejbkowicz et al. 1992). Bar, 10 µm.

 
One of the elements in the speckles that binds eIF4E could be capped RNA. To determine whether eIF4E is retained in the nucleus in an RNA-dependent manner, CV-1 cells were treated with RNase A and the localization of eIF4E was examined by immunofluorescence ( Fig. 4 B). RNase A treatment had no effect on the localization of eIF4E ( Fig. 4 B, a and d). As previously reported, the Sm ( Fig. 4 B, c and f), but not SC35 ( Fig. 4 B, b and e), distribution is dramatically changed after this treatment whereby it becomes diffuse in the nucleoplasm ( Spector et al. 1992). These results suggest that the localization of eIF4E in the speckles does not appear to require its association with RNA. However, this negative result needs to be interpreted with caution. RNase treatment might not be expected to remove eIF4E from the speckles, if when bound to RNA, eIF4E interacts with both RNA and other insoluble proteins or structural elements in the speckles.

The Nuclear Distribution of eIF4E Is Regulated by the Dual Specificity Kinase Clk/Sty
Splicing factors of the SR family are substrates for the dual specificity kinase, Clk/Sty ( Colwill et al. 1996). Expression of mammalian Clk/Sty wt in COS-1 cells causes the release of SR proteins from the speckles, whereas a catalytically inactive Clk/Sty does not ( Colwill et al. 1996; Caceres et al. 1998). To examine whether Clk/Sty also affects the localization of eIF4E, HeLa cells were transiently transfected with myc-Clk/Sty wt or the catalytically inactive myc-Clk/StyK190R, and the localization of eIF4E was determined by immunofluorescence. Expression of Clk/Sty wt, but not Clk/StyK190R, resulted in the disruption of the eIF4E speckles ( Fig. 5A and Fig. B, top). As previously reported, in transfected cells using anti-SC35 or the anti-SR mAb 104, which is specific for phosphorylated SR proteins and recognizes all SR family members ( Colwill et al. 1996; Caceres et al. 1998), Clk/Sty wt, but not the catalytically inactive kinase, released the SR splicing factors from the speckles ( Fig. 5A and Fig. B, middle and bottom; Colwill et al. 1996). Taken together, these results indicate that the nuclear localization of eIF4E is regulated by the dual specificity kinase, Clk/Sty.


Figure 5
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  		Figure 5 The nuclear localization of eIF4E is regulated by Clk/Sty. HeLa cells were transiently transfected with myc-Clk/Sty wild-type (A) or myc-Clk/StyK190R (B) for 24 h. The localization of endogenous eIF4E and SC35, or SR splicing factors was determined with mAbs 10C6, anti-SC35, and anti-SR 104, respectively, and revealed with Texas red-conjugated secondary antibody. The distribution of transiently transfected Clk/Sty was determined with a rabbit anti-myc antibody and fluorescein-conjugated secondary antibody. Images were taken from a 63x objective of a Zeiss LSM 410 microscope and analyzed with a Kontron IBAS confocal imaging system. Bar, 10 µm.

 

   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
Nuclear eIF4E is shown here to be distributed throughout the nucleoplasm, but is concentrated in speckles. Electron microscope studies identified the splicing factor-enriched speckles as clusters of interchromatin granules surrounded by perichromatin fibrils ( Spector 1993). The fluorescence from the speckles, as detected with antibodies against various splicing factors, such as SC35, corresponds to interchromatin granules and some perichromatin fibrils, whereas the more dispersed nucleoplasmic signal represents perichromatin fibrils, which are mostly localized throughout the nucleus ( Spector 1996). Although numerous studies have provided abundant information about the composition of the speckles, the precise function of these structures remains unclear. One model suggests that the speckles are splicing component storage sites, from which they are recruited to nascent pre-mRNA transcription sites ( Singer and Green 1997). This is consistent with the observations that most pre-mRNAs are spliced cotranscriptionally ( Beyer and Osheim 1988; Spector 1996; Sleeman and Lamond 1999), and that nascent RNA detected by short pulses of [3H]uridine primarily localize to the perichromatin fibrils and not to the interchromatin granules ( Spector 1996). The detection by in situ hybridization of several pre-mRNAs and corresponding spliced mRNAs within the speckles suggests that some splicing may also occur within these structures ( Xing et al. 1993, Xing et al. 1995).

In a cell permeabilization assay, eIF4E was specifically released from the nucleus by m7GpppG ( Fig. 4 A). One interpretation of this result could be that eIF4E is associated with pre-mRNA in the speckles, and that this association is disrupted by m7GpppG. This model is consistent with previous electron microscope studies demonstrating that the speckles contain poly A RNA ( Carter et al. 1991). Alternatively, it is possible that the speckled eIF4E is not associated with mRNA and that eIF4E in the speckles is released to the nucleoplasm as a result of its interaction with the excess cap analogue. This is consistent with the resistance of eIF4E in the speckles to RNase treatment. However, as indicated earlier, the resistance of eIF4E to RNase treatment is not definitive proof for the lack of association of eIF4E with mRNA. Taken together, our results suggests that eIF4E is retained in the speckles primarily through its association with proteins and not RNA.

The localization of eIF4E to the speckles raises the possibility of a role in mRNA processing. This is consistent with the observation that DRB causes the clustering of eIF4E speckles. DRB and {alpha}-amanitin inhibit RNA polymerase II transcription at the elongation step ( Cochet-Meilhac and Chambon 1974; Koumenis and Giaccia 1997; Zhu et al. 1997) and cause the rounding up of splicing factor speckles ( Spector et al. 1993; Spector 1996). It was suggested that the clustering of splicing factors results from the accumulation of splicing components at the storage sites in response to the reduction of pre-mRNA levels ( Spector 1996), and as a consequence, in less splicing. Accordingly, disruption of pre-mRNA splicing in vivo by microinjected oligonucleotides or antibodies against snRNAs also causes the clustering of splicing factors ( O'Keefe et al. 1994). This explanation could also apply to eIF4E as a potential component of the splicing machinery.

Since U1 and U2 snRNAs colocalize with snRNPs and SC35 ( Huang and Spector 1992), eIF4E could, in principle, also be associated with the trimethylated cap (m3G) of UsnRNAs in the speckles. However, this does not appear likely because UsnRNPs can be quantitatively immunoprecipitated from intact particles with an m3G-specific antibody ( Bringmann et al. 1983), indicating that this structure is not sequestered by proteins ( Kramer et al. 1984). Also, the affinity of eIF4E for the trimethylated cap structure is tenfold less than that for the monomethylated cap ( Wieczorek et al. 1999). Additionally, the m3G cap is a poor inhibitor of cap-dependent translation and substitution of the monomethylated cap with the trimethylated cap on β-globin mRNA reduced its translation by fourfold ( Darzynkiewicz et al. 1988).

Expression of the Clk/Sty kinase in COS-1 cells causes the release of the ASF/SF2 SR protein from the speckles ( Colwill et al. 1996). The dual specificity Clk/Sty kinase phosphorylates the serine/arginine-rich region (RS domain) of SR splicing factors. Phosphorylation of this domain is required for the activation of SR splicing factors ( Mermoud et al. 1994) and has been implicated in protein–protein interactions ( Kohtz et al. 1994). Since Clk/Sty interacts with ASF/SF2 through its RS domain, it may also associate and phosphorylate other constituents of the speckles and participate in a more general mechanism of splicing activation. Expression of Clk/Sty wt, but not of Clk/StyK190R, dispersed the nuclear eIF4E speckles. These results suggest that the nuclear localization of eIF4E is dependent on the distribution of SR splicing factors or, more generally, on splicing activity. Since eIF4E does not contain an RS motif, the identification of nuclear eIF4E-binding proteins should be helpful in establishing the mechanism by which Clk/Sty releases eIF4E from the speckles.

Since our results raise the possibility that eIF4E is associated with splicing factors, eIF4E could be involved in splicing and/or mRNA export. There is persuasive evidence that the nuclear CBC is important for mRNA splicing ( Izaurralde et al. 1994; Lewis et al. 1996) and UsnRNA export ( Izaurralde et al. 1995). eIF4E perhaps could be required for the processing of a specific subset of mRNAs. eIF4E may be colocalized with splicing components and regulated in a similar fashion to prevent its association with the monomethylated cap structure of snRNAs. A subset of snRNAs, including U1, U2, U4, and U5, are transcribed by RNA polymerase II and acquire a monomethylated cap structure before their export to the cytoplasm for hypermethylation ( Huber et al. 1998). As these snRNAs are not spliced, targeting of eIF4E to pre-mRNAs through its interaction with specific splicing factors could provide an effective way to prevent the tethering of snRNAs to the translation machinery.

Previous studies showed that overexpression of eIF4E in NIH3T3 cells increases cyclin D1 expression by stimulating its mRNA export ( Rousseau et al. 1996). However, it is not known whether eIF4E stimulates cyclin D1 mRNA export directly through its association with the cap in the nucleus, or indirectly by increasing the levels of a protein involved in the export of this mRNA. Nevertheless, it seems reasonable that once associated with the cap, eIF4E will accompany the RNA to the cytoplasm. Since eIF4E is retained in rounded up speckles after transcription inhibition and is colocalized with splicing factors, it remains to be determined whether eIF4E binds to the cap early during RNA polymerase II transcription, and whether it could be involved in 3' mRNA processing, splicing, and nucleocytoplasmic transport of mRNA.


   Acknowledgments
 
We thank J.C. Bell and T. Maniatis for providing plasmids and antibodies. We are indebted to Colin Lister for excellent technical assistance and H. Dilhuidy for technical support with the confocal microscopy. We thank B.J. Blencowe and A.R. Krainer for comments on the manuscript, and members of our laboratory, R.H. Singer, S.A. Adam, and J. Pelletier for interesting discussions.

J. Dostie was supported by a studentship from the Medical Research Council of Canada. F. Lejbkowicz was supported by a fellowship from Israel Cancer Research Fund (Montréal). This work was supported by a grant from the Medical Research Council of Canada to N. Sonenberg. N. Sonenberg is a Distinguished Scientist of the Medical Research Council of Canada and a Howard Hughes Medical Institute International Scholar.

Submitted: 27 September 1999

Revised: 11 November 1999

Accepted: 1 December 1999

Josée Dostie and Flavio Lejbkowicz contributed equally to this work.

Flavio Lejbkowicz's present address is Department of Microbiology, Rambam Medical Center, Haifa, Israel 31096.

Abbreviations used in this paper: CBC, cap-binding complex; DRB, 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole; eIF4E, eukaryotic initiation factor 4E; RT, room temperature.


   References
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 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

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