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© The Rockefeller University Press,
0021-9525/2000//13 $5.00
The Journal of Cell Biology, Volume 149, Number 1,
, 2000 13-16
Mini-Review |
Pores in the Wall
: Claudins Constitute Tight Junction Strands Containing Aqueous Pores
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.81-75-753-466081-75-753-4372
© 2000 The Rockefeller University Press
In vertebrates, tight junctions (TJs) play a central role in sealing the intercellular space in epithelial and endothelial cellular sheets (Schneeberger and Lynch 1992; Gumbiner 1993; Anderson and van Itallie 1995). Without TJs, these cellular sheets cannot function as barriers to establish compositionally distinct fluid compartments. To maintain homeostasis, various materials must be selectively transported across these cellular sheets. Two distinct pathways are known for this transport: the transcellular and paracellular pathways in which materials move across plasma membranes and TJs, respectively (Spring 1998; Fig. 1). The molecular machinery involved in transport through the transcellular pathway (i.e., channels, pumps, transporters, etc.) has been identified and well characterized in molecular terms. In sharp contrast, our knowledge regarding paracellular transport is limited mainly because of the lack of information on the molecular architecture of TJs. However, recent identification of TJ-specific integral membrane proteins prompted us to consider the molecular mechanism behind the barrier of TJs as well as transport across TJs.
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Claudins Constitute Paired TJ Strands |
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 Top Claudins Constitute Paired TJ... The Claudin Family, A...Claudins Are Functionally...Aqueous Pores within TJ...PerspectiveReferences |
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65-kD integral membrane protein with four transmembrane domains, was identified as the first component of TJ strands (Furuse et al. 1993; Ando-Akatsuka et al. 1996), this identification was regarded as the Holy Grail in this field (Gumbiner 1993), and successive studies emphasized the importance of occludin in the structure and functions of TJs (Balda et al. 1996; McCarthy et al. 1996; Chen et al. 1997; Wong and Gumbiner 1997). However, it has gradually become clear that the molecular architecture of TJ strands is more complex than expected (Balda et al. 1996; Hirase et al. 1997; Moroi et al. 1998). Especially, the fact that the occludin-deficient visceral endoderm cells still bear well developed network of TJ strands indicated that, as yet, unidentified membrane proteins or lipid molecules constitute TJ strands (Saitou et al. 1998).
In 1998, two novel integral membrane proteins (23 kD) with four transmembrane domains were identified from the junction-enriched fraction as components that copartitioned with occludin (Fig. 2; Furuse et al. 1998a). These proteins, named claudin-1 and -2, were structurally related (30% identical at the amino acid sequence level), but showed no sequence similarity to occludin. The most characteristic feature of these molecules was that they reconstituted TJs in fibroblasts when singly transfected (Furuse et al. 1998b). The reconstituted TJs were morphologically indistinguishable from those in situ, although they were not zonula but puncta occludens; ultrathin and freeze-fracture replica electron microscopy identified the formation of kissing points and paired strands, respectively, between adjacent transfectants (Furuse et al. 1998b; Kubota et al. 1999).
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The Claudin Family, A Newly Emerging Gene Family |
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TopClaudins Constitute Paired TJ...The Claudin Family, A...Claudins Are Functionally...Aqueous Pores within TJ...PerspectiveReferences |
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Northern blotting showed that tissue distribution patterns of each claudin member are distinct (Furuse et al. 1998a; Morita et al. 1999a). For example, claudin-1 and -2 are expressed at high levels in the liver and kidney, whereas claudin-3 mRNA is detected mainly in the lung and liver. Claudin-4, -7, and -8 are primarily expressed in the lung and kidney. These findings together with those of immunofluorescence microscopy suggested that, in various tissues, >2 species of claudins are coexpressed in single cells. On the other hand, it also became clear that some types of cells express their own specific claudin species. One example is claudin-5/TMVCF, which consists of TJ strands specifically in endothelial cells of blood vessels (Morita et al. 1999c). To date, claudin-5/TMVCF has not been found in nonendothelial cells. Another example is claudin-11/OSP, which is primarily expressed in the brain and testis. This claudin species was shown to constitute TJ strands between lamellae of myelin sheaths of oligodendrocytes in the brain and those between adjacent Sertoli cells in the testis (Morita et al. 1999b). Recently, claudin-11/OSP–deficient mice were successfully generated (Gow et al. 1999). In these mice, TJ strands were absent in myelin sheaths of oligodendrocytes and Sertoli cells, conclusively indicating that, in these types of cells, TJ strands are mainly composed of a single specific claudin, claudin-11/OSP. These findings naturally raised questions regarding the physiological relevance of the existence of so many claudin species. This point will be discussed later.
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Claudins Are Functionally Involved in the TJ Barrier |
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TopClaudins Constitute Paired TJ...The Claudin Family, A...Claudins Are Functionally...Aqueous Pores within TJ...PerspectiveReferences |
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Recently, to solve these technical difficulties, the bacterial peptide toxin, Clostridium perfringens enterotoxin (CPE), was used (Sonoda et al. 1999). As described above, claudin-4 was initially identified as a CPE receptor (CPE-R), and it was reported that the COOH-terminal half of this peptide (C-CPE) specifically bound to claudin-4/CPE-R (Katahira et al. 1997). When this C-CPE was applied to cultured epithelial cells (i.e., MDCK cells that express mainly claudin-1 and -4), claudin-4 was specifically removed from TJs with a concomitant decrease in transepithelial electrical resistance as well as in the number of TJ strands. These findings provided concrete evidence of the direct involvement of claudins in the barrier functions of TJs.
We have now concluded that claudins constitute the wall between adjacent epithelial/endothelial cells, which is responsible for sealing in the paracellular pathway. However, as noted above in situ, this wall contains pores, leading to the question of the chemical natures of these pores.
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Aqueous Pores within TJ Strands |
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TopClaudins Constitute Paired TJ...The Claudin Family, A...Claudins Are Functionally...Aqueous Pores within TJ...PerspectiveReferences |
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It is possible that only claudin-16/paracellin-1, but not other claudins, can form pores through its homotypic interaction on the claudin-based wall. However, as the permeability properties of individual paired TJ strands appear to be fairly variable in different epithelia (Diamond 1977; Gumbiner 1993), it is more likely that most claudin species can constitute not only the wall, but also the pores in the wall. Recent detailed analyses of the manner of interaction of heterogeneous claudin species within and between TJ strands provided important information to clarify this point (Furuse et al. 1999). (1) When two of claudin-1, -2, and -3 were coexpressed in L fibroblasts, claudins were copolymerized into individual TJ strands (heteropolymers) in any combination (Fig. 3 a). (2) When two of the L transfectants singly expressing claudin-1, -2, or -3 were cocultured, claudin-3–based strands (homopolymers) laterally associated with claudin-1– or claudin-2–based strands (homopolymers) in a heterotypic manner, but such heterotypic paired strands were not formed between claudin-1– and claudin-2–based strands (Fig. 3 b). (3) When L transfectants singly expressing claudin-1 were cocultured with those coexpressing claudin-1 and -2, claudin-1 homopolymers laterally associated with claudin-1/2 heteropolymers to form paired strands (Furuse, M., and S. Tsukita unpublished data; Fig. 3 c). These observations suggested a possible explanation as to how claudins constitute the wall and the pore simultaneously. In Fig. 4, two types of paired strands are supposed, claudin-1/3–based and claudin-1/2–based paired strands, in which individual strands, i.e., heteropolymers consisting of claudin-1 and -3 or claudin-1 and -2, respectively, are laterally associated. In the former type of paired strands, claudin-1 and -3 could adhere both in homotypic and heterotypic manners (Fig. 3 b). However, in the latter type, claudin-1 could not adhere with claudin-2 in a heterotypic manner, resulting in the formation of pores within paired strands. This is purely speculative at this stage, but as in situ TJ strands are composed of various combinations of at least 18 claudin species, it is tempting to hypothesize that the tightness of paired TJ strands is determined by the number/type of species of claudins and their mixing ratio in strands.
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Perspective |
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TopClaudins Constitute Paired TJ...The Claudin Family, A...Claudins Are Functionally...Aqueous Pores within TJ...PerspectiveReferences |
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The tightness of TJs is determined not only at the level of individual strands, but also at that of strand networks (Claude and Goodenough 1973; Madara and Dharmsathaphorn 1985). The number of strands as well as the frequency of their ramification have been shown to be important factors to determine the tightness of TJs. However, it remains unclear as to what regulates these factors in molecular terms. As the network pattern of reconstituted claudin-based strands in L fibroblasts differs significantly depending on claudin species, the combination and the mixing ratio of claudin species appear to also be crucial in regulating the tightness of TJs at the level of the network (Furuse et al. 1998b; Morita et al. 1999b,Morita et al. 1999c). In this connection, the regulation of individual claudin species should be examined in detail not only at the transcriptional, but also at the posttranscriptional levels. We are now in a position to begin exploring the molecular mechanism behind the determination and regulation of tightness of TJs (i.e., the transport of materials across TJs).
Submitted: 31 January 2000
Revised: 18 February 2000
Accepted: 23 February 2000
Abbreviations used in this paper: CPE, Clostridium perfringens enterotoxin; TJ, tight junction.
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