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© The Rockefeller University Press,
0021-9525/2000//77 $5.00
The Journal of Cell Biology, Volume 150, Number 1,
, 2000 77-88
Original Article |
The Unfolded Protein Response Regulates Multiple Aspects of Secretory and Membrane Protein Biogenesis and Endoplasmic Reticulum Quality Control
dtn1{at}psu.edu
The unfolded protein response (UPR) is an intracellular signaling pathway that relays signals from the lumen of the ER to activate target genes in the nucleus. We devised a genetic screen in the yeast Saccharomyces cerevisiae to isolate mutants that are dependent on activation of the pathway for viability. Using this strategy, we isolated mutants affecting various aspects of ER function, including protein translocation, folding, glycosylation, glycosylphosphatidylinositol modification, and ER-associated protein degradation (ERAD). Extending results gleaned from the genetic studies, we demonstrate that the UPR regulates trafficking of proteins at the translocon to balance the needs of biosynthesis and ERAD. The approach also revealed connections of the UPR to other regulatory pathways. In particular, we identified SON1/RPN4, a recently described transcriptional regulator for genes encoding subunits of the proteasome. Our genetic strategy, therefore, offers a powerful means to provide insight into the physiology of the UPR and to identify novel genes with roles in many aspects of secretory and membrane protein biogenesis.
Key Words: protein translocation • protein maturation • gene regulation • glycosylation • protein degradation
© 2000 The Rockefeller University Press
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Introduction |
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 TopAbstract Introduction Materials and MethodsResultsDiscussionReferences |
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The UPR is a ubiquitous mechanism observed in all eukaryotic organisms from humans to yeast (reviewed in Chapman et al. 1998; Kaufman 1999). An important step in uncovering the mechanisms underlying the UPR came from promoter studies of the known target gene, KAR2. Deletion analysis revealed specific promoter elements termed UPREs (unfolded protein response elements) that are required for regulated gene activation (Mori et al. 1992; Kohno et al. 1993). The UPRE defined from yeast KAR2 when combined with the CYC1 TATA box is sufficient to drive the UPR-dependent expression of a heterologous reporter gene. This result was key to a genetic strategy for isolating mutants defective for signaling through the UPR (Cox et al. 1993; Mori et al. 1993). The first gene identified encodes an ER transmembrane protein with a cytosol-facing serine/threonine kinase, Ire1p (also termed Ern1p), which acts as a sensor of the ER lumen. Through an unknown mechanism, stimuli such as accumulation of misfolded proteins cause Ire1p to oligomerize and transautophosphorylate as a prerequisite step for activation (Shamu and Walter 1996). Hereafter, the mechanism of signal transduction diverges radically from paradigms derived from studies of plasma membrane receptors. Upon activation, a cytosolic nuclease domain of Ire1p excises an intron from HAC1 mRNA (Cox and Walter 1996; Kawahara et al. 1997), which encodes a UPRE-specific transcription activator (Cox and Walter 1996; Mori et al. 1996). tRNA ligase then joins the two exons, thereby completing the splicing of HAC1 mRNA by a nonconventional, nonspliceosomal mechanism (Sidrauski et al. 1996). Unspliced HAC1 mRNA is stable in cells and initiates translation, but the presence of the intron stalls ribosomes so that no Hac1p is produced (Chapman and Walter 1997). Removal of the intron upon UPR activation relieves the translational block to allow synthesis of Hac1p and the induction of target genes; the Ire1p-mediated splicing reaction, therefore, is a key regulatory step in the pathway.
Despite the detailed understanding of the mechanisms surrounding UPR signaling and gene regulation, the precise physiological role of the pathway has remained largely unexplored. The most extensive list of targets was assembled from yeast and includes KAR2, LHS1, FKB2, PDI1, EUG1, and ERO1. The products of these genes localize to the ER lumen and catalyze protein folding. KAR2 and LHS1 encode proteins with similarity to the Hsp70 class of molecular chaperones (Normington et al. 1989; Rose et al. 1989; Craven et al. 1996). FKB2 is a prolyl isomerase homologue (Partaledis and Berlin 1993). PDI1, EUG1, and ERO1 promote disulfide bond formation (LaMantia et al. 1991; Tachibana and Stevens 1992; Craven et al. 1996; Pollard et al. 1998). Thus, the UPR regulates the abundance of ER resident chaperones and other enzymes required for folding, assembly, and modification of secretory and membrane proteins.
The initial identification of IRE1 as a component of UPR signaling provided additional clues. IRE1 was first reported as a gene required for inositol prototrophy (Nikawa and Yamashita 1992). It was later found that regulation of the inositol biosynthetic pathway requires a functional UPR (Cox et al. 1997). The observation showed that the inositol pathway interacts intimately with the UPR. Since inositol biosynthesis and other aspects of lipid biosynthesis are coregulated, these observations suggest that the UPR is involved in the regulation of membrane biosynthesis. Such a connection may serve to expand the ER, when more ER resident proteins need to be accommodated as the result of UPR induction.
Proteins that enter the ER and cannot be folded correctly, even after boosting ER folding capacity through UPR induction, are degraded. The degradation pathway, termed ER-associated protein degradation (ERAD; reviewed in Sommer and Wolf 1997; Brodsky and McCracken 1999), translocates misfolded proteins back into the cytosol, where they are degraded by the proteasome. Retrotranslocation (also called dislocation) is thought to utilize the same core protein complex (Sec61p and associated subunits) that forms the protein conducting channel in the translocon through which proteins are delivered to the ER lumen. Conceptually, the UPR, in its previously known scope, and ERAD provide different means of dealing with protein misfolding in the ER: the UPR by inducing enzymes thought to play a corrective role and ERAD to dispose of proteins that cannot be rescued. Here, and in a concomitant study (Travers et al. 2000), we show that the two pathways indeed are intimately linked and that the scope of the UPR encompasses many more aspects of protein maturation and ER quality control than previously appreciated.
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Materials and Methods |
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Construction of pDN336 and pDN388.
The plasmid pDN336 used as the reporter in strain DNY421 was constructed by inserting the full-length IRE1 gene, released from pCS110 (Cox et al. 1993) as an XhoI/BamHI fragment, and the full-length ADE3 gene, released as a BamHI/NheI fragment, into XhoI/XbaI sites of the yeast shuttle vector, pRS316 (URA3, CEN6, ARSH4; Sikorski and Hieter 1989). Both the XbaI and the NheI sites were destroyed in the construction. pDN388 is similar, except that the insert is in the pRS315 vector (LEU2, CEN6, ARSH4; Sikorski and Hieter 1989).
pDN390.
The plasmid, pJC835, containing the HAC1i gene in the shuttle vector, pRS313 (Cox and Walter 1996), was digested with AlwNI and NgoMI to release the gene. The fragment was ligated into pRS315 digested with the same sites.
CPY*HA Expression Vectors.
The prc1-1 allele expresses the variant CPY* as a glycine to arginine change at position 255 (Finger et al. 1993). We constructed an HA epitope-tagged version of CPY* by site-directed mutagenesis using a PCR-based approach. The PRC1 gene was first amplified as two fragments. The N-proximal fragment amplified with the primers 5'-CCATCGAATTCCGTATAT-3' and the phosphorylated primer 5'-GAAATCTTGGCCCTTGTTGACG-3' using Vent polymerase (New England Biolabs). This fragment includes the PRC1 promoter and coding sequences to amino acid position 251. C-proximal sequences were amplified using the phosphorylated primer 5'-CACATCGCTAGAGAATCCTACGCC-3' incorporating a glycine to arginine change at amino acid 251 and the primer 5'-CCCTCTAGACTAACCCAAAGAAGCGTAATCTGGAACATCATATGGGTATAAGGAGAAACC-ACCG-3', fusing the HA epitope tag, a termination codon, and an XbaI site. The C-proximal fragment was amplified using Taq polymerase since the primer combination precluded amplification by Vent polymerase under all conditions attempted. Blunt ends were generated for this fragment using T4 DNA polymerase. The N-proximal fragment was digested with EcoRI, the C-proximal with XbaI, and both fragments ligated into the vector pDN201 (Ng et al. 1996) digested with same enzymes. The mutant gene in pDN431 was confirmed by DNA sequence analysis. A second version of the plasmid, pDN436, was constructed by releasing the CPY*HA gene from pDN431 as a SalI/EcoRI (blunt) fragment and ligation into the SalI/SmaI sites of pRS315 (Sikorski and Hieter 1989).
Genetic Screen
The reporter strain for the screen was constructed by first crossing JC147 and EY0060. Diploids derived were sporulated, tetrads dissected, and haploids screened for the following genotype: MATa, ire1::TRP1, ade2, ade3, UPRE-LacZ::HIS3. One such strain was isolated and transformed with pDN336 to create DNY421.
For mutagenesis, 50 A600 OD units of DNY421 cells were washed once in sterile water and resuspended in 25 ml 0.9% KCl. With constant stirring, the cells were given four 30-s pulses of short wave UV light from a source mounted 15 cm above. After each pulse, an aliquot was removed, a portion serially diluted, and spread onto YPD plates to determine the kill rate. The remainder of each aliquot was pelleted and resuspended in 10 ml YPD media and allowed to recover for 18 h on a roller drum. All procedures to this point were performed in a darkroom and incubations at 30°C. Cells receiving a UV dose of 120 s resulted in a kill rate of 62% and were used for the screen. Mutagenized cells were spread onto YPD plates lacking additional adenine. Nonsectoring colonies were picked and rescreened by restreaking for single colonies (see Results for specific numbers). Isolates were backcrossed to DNY420 and recessive mutants determined by the restoration of the colony sectoring phenotype of their respective heterozygote diploids. Tetrad dissection was performed to determine the allelic complexity of the mutants. Only those exhibiting a 2:2 segregation pattern indicating a single mutant gene were continued. All strains were backcrossed at least twofold before further analysis.
Complementation tests were performed to determine the number of genes represented in the initial group of 20. Complementing strains were scored as those giving rise to colony sectoring diploids. However, before the completion of all possible crosses, many strain combinations gave rise to nonsectoring diploids indicating noncomplementation and therefore suggesting different alleles of the same gene. Upon tetrad analysis of the diploids, it was determined that, in most cases, mutant alleles were unlinked. The criterion for linkage was 4:0 segregation of mutant to wild-type spores. Tetrad analysis was performed for all possible crosses for the assignment of linkage groups.
Cloning and Identification of PER Genes
Two general approaches were employed to clone PER (protein processing in the ER) genes. The first takes advantage of the counterselectable marker, URA3, contained in the pDN336 (Boeke et al. 1984). Since the growth of per mutants are dependent on the IRE1, cells losing pDN336 fail to grow on media containing 5-fluoroorotic acid (5-FOA). Complementation of mutant alleles alleviates the requirement for IRE1 and thus allows for selection of complementing plasmids from genomic libraries. The PER5 and PER8 genes were cloned using this first approach.
Mutant cells were transformed with a yeast genomic library based on the multicopy YEp13 vector (Lagosky et al. 1987). After transformation, the cells were grown overnight in SC-Leu media allowing transformants receiving complementing plasmids to lose pDN336. Transformants were later plated and incubated on SC-Leu media containing 5-FOA (1 mg/ml) at 30°C. Plasmids were recovered from 5-FOA resistant isolates by zirconium bead disruption and purification using the Wizard Miniprep kit (Promega). Plasmid amplification was performed after transformation into bacterial DH5
cells. Retransformation of the recovered plasmids into the respective mutant strains was typically carried out to assess complementation of the sectoring phenotypes. Although this approach worked well for PER5 and PER8, it was less successful for other strains attempted. The high incidence of false positives due to plasmids carrying truncated IRE1 contained in the library added an additional layer to the procedure that was overly time consuming.
The second approach scored for complementation of the sectoring phenotype using a low copy genomic library (used for cloning of PER2, PER4, PER13, and PER16). The library, based on YCp50 (Rose et al. 1987), required that the reporter pDN336 be swapped for pDN388 to be compatible. Mutant strains transformed by the library were spread onto SC-Ura/adenine-limiting (6 µg/ml) plates at low density (400 cfu/plate) to develop the colony color phenotype. Typically, between 10,000 and 25,000 transformants were screened for restoration of sectoring. Positives were cured of the reporter and complementing plasmids were recovered as described above. Recovered plasmids were transformed back into the respective mutant strains to confirm complementation.
For all clones, DNA sequence analysis was performed to determine the identity of inserts (Nucleic Acid Facility, Pennsylvania State University, University Park, PA). The sequences of vector/insert junctions were obtained using the primers N168 (5'-CGCTACTTGGAGCCACTATCGAC-3') and N169 (5'-ATCGGTGATGTCGGCGATAT-3'). Junction sequences were submitted to the Saccharomyces Genome Database (http://genome-www.stanford.edu/Saccharomyces/) to obtain complete insert sequences and identities of open reading frames. The insert sequences were used to facilitate deletion mapping using standard recombinant DNA methods to determine specific complementing genes. Subclones of genes derived from the high copy library (YEp13) were inserted into centroplasmic plasmids to assess complementation.
Cell Labeling and Immunoprecipitation
Typically, 3 A600 OD units of log phase cells were pelleted and resuspended in 0.9 ml of SC media lacking methionine and cysteine. After 30 min of incubation at the appropriate temperature, 150 µCi of [35S]Met/Cys (Pro-mix; Amersham Pharmacia Biotech) is added to cells for 5 or 10 min. A chase, where appropriate, was initiated by adding cold methionine/cysteine to a final concentration of 2 mM. Cell labeling/chase was terminated by the addition of trichloroacetic acid to 10%. Cells were homogenized by the addition of 0.4 ml of 0.5-mm zirconium beads, followed with agitation in a Mini-bead beater cell disrupter (Biospec Products). The homogenate was transferred to a fresh tube and pooled with a subsequent 10% TCA bead wash. After centrifugation, the TCA precipitate pellet was resuspended in 120 µl of IPS II (100 mM Tris base, 3% SDS, 1 mM PMSF) and heated to 100°C for 5 min. Insoluble debris was pelleted and 40 µl of the detergent lysate was added to 560 µl IPS II (1% Triton X-100, 50 mM Tris, pH 7.5, 1 mM PMSF, 1 µl yeast protease inhibitor cocktail; Sigma-Aldrich) and the appropriate antiserum. After a 2-h incubation at 4°C, the sample was centrifuged for 10 min at 16,000 g and the supernatant transferred to a fresh tube containing protein A–Sepharose beads. The tube was rotated for 30 min and washed three to five times with IPS I (0.2% SDS, 1% Triton X-100, 50 mM Tris, pH 7.5) and once with PBS. Immunoprecipitated proteins were eluted with gel sample buffer, separated by gel electrophoresis, and visualized by autoradiography.
In pulse-chase assays to determine protein stability, immunoprecipitations were normalized by measuring TCA-precipitable counts using an LS5801 scintillation counter (Beckman Coulter) and adjusting lysates for equal counts.
Northern Blot Analysis
Preparation of RNA, gel electrophoresis, blot transfer, and probing were performed as described in Cox and Walter 1996. RNA from UPR-induced cells were treated for 60 min with 2.5 µg/ml tunicamycin (Sigma-Aldrich) before RNA isolation. All templates for probes were prepared by PCR amplification of genomic DNA. Probes were prepared by random primer extension labeling using [32P]-dCTP. The ACT1 template is a 600-bp fragment corresponding to the 3'-end of coding sequences. The KAR2 template is a 600-bp fragment corresponding to the 5'-end of coding sequences. Two RFT1 templates were prepared and used independently. One includes 600 bp of the 5'-coding sequences and the other includes 700 bp of the 3'-coding sequences.
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Results |
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We performed the screen using a yeast colony color sectoring assay (Koshland et al. 1985). Yeast cells harboring an ade2 mutation give rise to red colonies when grown on media limiting for adenine. If a strain contains both mutant ade2 and ade3 alleles, the red pigment is not synthesized, and colonies are white. We constructed an ade2 ade3 mutant strain that is deleted for IRE1 (DNY421, Table ). This strain grows well on rich media. In addition, we constructed a centromeric reporter plasmid, pDN336, containing IRE1 and ADE3, which was transformed into DNY421 cells. Since ADE3 and IRE1 are not essential, the plasmid is lost at a frequency of 
10–2 per cell division (Guthrie and Fink 1991). For this reason, DNY421 cells gives rise to red and white sectored colonies, reflecting a mixed population of cells with and without the plasmid. Because IRE1 is required for activation of the UPR pathway, mutants that require UPR activation for growth cannot lose the IRE1-bearing plasmid and hence, are expected to give rise to red nonsectored colonies.
DNY421 cells were mutagenized with ultraviolet light to 62% lethality. We screened 50,000 colonies and isolated 101 nonsectoring and normally growing colonies. Of these, 40 were backcrossed to the parental strain, yielding 32 recessive, 2 dominant, and 6 sterile isolates. The dominant and sterile mutants were discarded. Heterozygous diploids generated from recessive mutants were sporulated and subjected to tetrad analysis to determine the segregation patterns. Of the diploids, 25 were sporulation-competent. Of those, 15 segregated 2:2 with a synthetic lethal phenotype and 5 with a synthetic negative growth phenotype (spores containing both mutations were viable, but displayed severely reduced growth rates), indicative of a single gene being responsible for the mutant phenotype. Of the remaining mutants, five exhibited other patterns and were discarded.
When crossed to each other, many of the mutants exhibited partial sectoring phenotypes that precluded clear determination of complementation. This was not a general effect of ploidy since all 20 mutants, when crossed to the parental strain, formed sectored colonies unambiguously. Further analysis demonstrated that most mutant genes are unlinked. These observations suggest some combinations of mutations display the genetic interaction termed unlinked noncomplementation. Although the significance for this group remains to be determined, unlinked noncomplementation has been used to help identify genes of the same function or pathway (Stearns and Botstein 1988). To group the 20 mutants, we crossed individual mutant strains and systematically subjected the resulting diploids to tetrad analysis. Most crosses failed to display linkage between the mutant alleles, indicating that the mutations were in different genes. This analysis defined 16 linkage groups indicating at least as many genes (Table ). We refer to the genes defined by the mutants as PER (protein processing in the ER) because most mutant alleles can be shown to affect various aspects of ER protein biogenesis and quality control (see below). The high fraction of per mutants represented by a single allele indicates that the screen is far from saturated.
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ire1 cells covered by pDN336 bearing wild-type IRE1). Cells were then scored for improved growth after loss of pDN336, obtained by screening for white, nonsectoring colonies. Using these criteria, all but two mutants (per1-1 and per15-1) could be relieved of the requirement for IRE1 function by directly activating the pathway (Table ). After crosses of the two remaining mutants (DNY523 and DNY540) with hac1 cells were sporulated, however, tetrad analysis revealed clear synthetic negative phenotypes for both mutants (data not shown). Thus, taken together, the results indicate that the synthetic defect of all 16 per mutants is due to the loss of the UPR pathway, rather than to the loss of a yet uncharacterized function of IRE1 unrelated to UPR signaling. To address the second criterion for physiological relevance, we determined the extent of constitutive UPR activation in mutant cells. The parental strain (DNY421) used in this study contains a genomic copy of a lacZ reporter gene fused to a minimal UPR promoter (Cox et al. 1993). Thus, β-galactosidase activity can be used to monitor activation of the pathway. Mutant cells were grown to early log phase, and cell extracts were prepared to measure β-galactosidase activity with the substrate 2-nitrophenyl-β-D-galactopyranoside (Cox et al. 1993). As shown in Fig. 1, with the exception of per16-1, all per mutants express β-galactosidase activity above wild-type levels, indicating induction of the UPR. Interestingly, the degree of UPR induction among the mutants varies widely, indicating that the UPR can be gradually modulated depending on the physiological needs of the cell.
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Wild-type (W303) and per mutant cells were metabolically pulse-labeled with [35S]amino acids for five minutes and chased with an excess of unlabeled methionine and cysteine for 30 min to allow for full processing of Gas1p (Fig. 2 A, lanes 2–22). As shown in Fig. 2, no preGas1p was observed in any lane, indicating that none of the per mutants displayed severe defects in protein translocation. However, about half of the mutants are defective in processing to the mature 125 kD form. These results indicate defects in ER protein processing functions that may include glycosylation, folding, GPI-anchor addition, or transport.
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As shown in Fig. 2 B, mutants per3-1, per5-1, per6-1, per6-2, per12-1, and per14-1 exhibited CPY underglycoslyation indicative of defective N-linked glycosylation. Correspondingly, these mutants synthesized forms of Gas1p with altered mobility expected for glycosylation mutants (Fig. 2 A). The size of the underglycosylated forms indicates that most of CPY is proteolytically processed to the mature form in each of the mutants, indicating that these mutants are not generally defective in protein transport from the ER to the Golgi apparatus.
The PER5/RFT1 Gene Is a Novel UPR Target Required for N-linked Glycosylation
We chose to analyze per5-1 in greater detail because it exhibited the most severe glycosylation defect. To this end, we analyzed CPY by pulse-chase analysis, followed by endoglycosidase H digestion in wild-type and per5-1 cells. As shown in Fig. 3 A, proCPY recovered after the pulse migrated as multiple species with altered mobility in per5-1 cells as compared with the P1 species from wild-type cells (Fig. 3 A, compare lanes 1 and 4). Removal of the sugar chains revealed that the heterogeneity was exclusively due to differences in glycosylation, as the deglycosylated forms comigrated (Fig. 3 A, lanes 7–12). The kinetics of processing to the mature form(s) was similar in both strains, indicating that folding and transport functions are intact in per5-1 cells. Similar results were obtained for Gas1p (Fig. 3 B), indicating that this defect is not substrate-specific.
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Inspection of the upstream sequences of PER5/RFT1 revealed a potential UPRE, suggesting that transcription of PER5 is regulated by the UPR (Fig. 4 A). To address this possibility directly, Northern analysis was performed using RNA extracted from control cells and from cells treated with the glycosylation inhibitor tunicamycin to induce UPR-regulated genes. As shown in Fig. 4 B, PER5/RFT1 is elevated in tunicamycin-treated cells (lanes 1–4). Quantitative analysis showed a moderate 2.5-fold induction. This is in contrast to KAR2, which was induced over 8-fold (Fig. 4 B). Transcriptional regulation of PER5/RFT1, as with KAR2, was dependent on the UPR, as no induction was detected in hac1 cells (Fig. 4 B, lanes 5 and 6).
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A Role for the UPR in ERAD
As many of the per mutants do not exhibit defects in Gas1p or CPY maturation, we wondered whether other aspects of ER function related to protein maturation are affected in some of the per mutants. To test whether genes encoding ERAD components were identified in this screen, we constructed a recombinant form of the ERAD substrate, CPY* (Finger et al. 1993), modified by addition of an HA epitope tag to simplify analysis. The new version, designated CPY*HA, behaved as a proper ERAD substrate: it was degraded rapidly in wild-type cells and was stabilized in a strain lacking the ERAD gene, CUE1 (Fig. 5; Biederer et al. 1997). We transformed each of the per mutants with pDN436, carrying the gene encoding CPY*HA. Pulse-chase analysis was used to assess substrate stability in each strain. Turnover of CPY*HA was delayed in per7-1, per8-1, per9-1, per13-1, and per16-1 (Fig. 5). Two mutants, per9-1 and per13-1, also displayed defects in Gas1p processing, indicating that their defects are pleiotropic. Their ERAD phenotypes may therefore be indirect. In all other per strains, including those displaying severely impaired protein processing, the rate of CPY*HA degradation was not altered (data not shown).
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cue1 strain. Plasmid pDN426 bearing SON1, completely complemented the per8-1 mutant. Moreover, CPY*HA was stabilized in son1 cells (data not shown), indicating that the defect per8-1 cells reflects a loss-of-function. Son1p was recently shown to be a transcription factor that regulates proteasome biogenesis (Mannhaupt et al. 1999), and son1 mutants are defective in cytosolic protein degradation (Johnson et al. 1995). Thus, a role for Son1p in ERAD is quite plausible. We cloned PER16 by screening for restoration of the sectoring phenotype after a plasmid shuffle of the URA3-marked reporter for LEU2 (pDN388) and transformation of the library. Subcloning of the inserts of plasmids that restored the sectoring phenotype of per16-1 cells identified UBC7, which was sufficient to complement the per16-1 mutation. UBC7 encodes a ubiquitin-conjugating enzyme that was previously shown to participate in ERAD (Biederer et al. 1996; Hiller et al. 1996).
The isolation of ERAD mutants in this screen suggested a tight physiological link between the UPR and ERAD. We explored this notion further by monitoring the fate of CPY*HA in a UPR-deficient strain. As shown in Fig. 6 A, CPY*HA was stabilized in ire1 cells (Fig. 6 A, lanes 5–8), whereas wild-type control cells degraded the substrate rapidly. Surprisingly, we also observed the appearance of a faster migrating band in ire1 cells that was not detected in control experiments performed with wild-type cells (Fig. 6 A, labeled preproCPY*HA). Its mobility and the timing of its appearance during the pulse suggested that the band represents the cytoplasmic, nontranslocated preproCPY*HA. To confirm this identity, we expressed CPY*HA in sec62-101 cells, in which translocation of CPY is severely impaired and thus accumulated cytosolic precursor proteins. The data in Fig. 6 B show that the preproCPY bands comigrate (compare lanes 2 and 3).
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ire1 strains to express epitope-tagged wild-type CPY (CPYHA) using the same vector as for CPY*HA. Expression of CPYHA caused no translocation defects in ire1 cells (Fig. 6 C), indicating that the defect results specifically from the expression of a misfolded protein that is a substrate of the ERAD pathway.
In ire1 cells expressing CPY*HA, we also observed a translocation defect for the endogenous protein Gas1p (Fig. 6 B, lanes 4 and 5), suggesting that the misfolded protein causes a general translocation defect in these cells. By contrast, ire1 cells alone or ire1 cells expressing CPYHA exhibit no defects in ER import of Gas1p (data not shown). As protein import into and export from the ER are thought to share the same translocation pore, it is possible that both processes directly compete for some limiting component(s) regulated by the UPR.
Quantitative analysis of the data in Fig. 6 A showed a delay of ER protein degradation in ire1 cells. In wild-type cells, 47, 13, and 3% of CPY*HA remained after 30, 60, and 90 minutes of chase, respectively. By contrast, in ire1 cells, 46, 37, and 22% of CPY*HA remained at the corresponding time points. This delay could result from two, possibly additive, effects: an impairment of import into the ER and a delay in reexport from the ER for degradation. Although the relative contributions of the two processes to the overall delay remains to be determined, ERAD function remains disrupted if the import block is alleviated (see below).
Taken together, the data suggest a role of the UPR in balancing the trafficking of proteins into and out of the ER during periods of stress as import and export substrates compete for a limiting core translocation machinery. The accumulation of CPY*HA would induce the UPR to augment the translocation machinery to clear the ER of the misfolded proteins while maintaining protein import into the ER. In support of this hypothesis, genes encoding components of the translocation machinery with roles for both import and export are targets of the UPR (Travers et al. 2000). In addition, CPY* and CPY*HA expression each cause UPR induction, albeit at a modest level of less than twofold as measured by a UPRE-lacZ reporter (Knop et al. 1996; Spear, E.D., and D.T.W. Ng, unpublished results).
To test this hypothesis, we assessed the contribution of two specific UPR target genes by increasing their expression as it would occur during normal UPR activation. To this end, we tested strains that contain duplicated genes encoding Kar2p or Sec61p, respectively. These genes were selected because of their dual roles in both ER protein import and ERAD (Pilon et al. 1997; Plemper et al. 1997; Zhou and Schekman 1999). In addition, both genes are inducible under conditions that activate the UPR (Travers et al. 2000). As shown in Fig. 7 A, increased gene dosage of either Kar2p or Sec61p was sufficient to alleviate the translocation defect for preproCPY*HA and preGas1p in ire1 cells. ire1 cells with increased expression of Kar2p also alleviated the delay in CPY*HA degradation (Fig. 7B and Fig. C). In contrast, ire1 cells containing an additional copy of SEC61 remain defective in degrading CPY*HA. The additional copy of SEC61 itself is not inhibitory to ERAD since wild-type cells under the same circumstances show normal if slightly accelerated ERAD function (Spear, E.D., and D.T.W. Ng, unpublished results). These results show that Kar2p becomes limiting for both translocation and ERAD when cells are challenged with CPY*HA, whereas Sec61p becomes limiting only for translocation, but not ERAD. Moreover, increased expression of either Kar2p or Sec61p can compensate for the translocation defect in ire1 cells. Taken together, these data demonstrate an important role for the UPR in regulating the import and export functions across the ER membrane.
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Regulation of Protein Glycosylation
Isolation of the per5-1 mutant led to the discovery of a new gene involved in protein glycosylation. We found that PER5 is upregulated by the UPR. PER5/RFT1 was originally cloned from a yeast mutant requiring the overexpressing of human p53 for viability (Koerte et al. 1995); as there is no yeast equivalent to mammalian p53, the reason for this phenotype and the function of PER5/RFT1 has remained obscure. Our studies demonstrate that PER5/RFT1 is required for efficient N-linked glycosylation of glycoproteins. PER5/RFT1 encodes a multispanning transmembrane protein that bears no significant sequence similarity to any other known component of the glycosylation machinery. Our results suggest that PER5/RFT1 functions in biosynthesis of the dolichol-linked carbohydrate precursor or in the protein conjugation step. Preliminary experiments indicate that per5-1 cells show an accumulation of a biosynthetic intermediate (Dol-PP-GlcNAc2-Man5) and a sharp decrease in the mature form (Dol-PP-GlcNAc2-Man9-Glc3; J. Helenius, D.T.W. Ng, P. Walter, and M. Aebi, unpublished results). These results suggest the intriguing possibility that PER5/RFT1 may encode the long sought-after flippase that translocates the Dol-PP-GlcNAc2-Man5 intermediate (which is synthesized on the cytosolic face of the membrane) to the lumenal face for further processing (Burda and Aebi 1999). Consistent with this view, those carbohydrates that are attached to proteins in per5-1 cells were found to be fully mature. Further experiments to address this notion are in progress.
Genome expression data has shown that additional components of the glycosylation biosynthetic machinery are regulated by the UPR (Travers et al. 2000). The regulation of glycosylation components helps cement our broadened view of the UPR, as it indicates a role of the pathway in adjusting the cell's biosynthetic capacity of the ER according to need. Similarly, we isolated per mutants defective in GPI anchorage, and many GPI biosynthetic genes are UPR targets (Travers et al. 2000).
UPR Regulation of ER Quality Control
The identification of ERAD genes suggested a tight physiological link between ERAD and the UPR. We have provided direct evidence for such a link using CPY*HA, which is stabilized in UPR-deficient cells. Consistent with previous studies (Kawahara et al. 1997; Zhou and Schekman 1999), we show that various ERAD-deficient mutants constitutively induce the UPR. As shown in the parallel study, most known ERAD genes are activated by the UPR (Travers et al. 2000). Moreover, in this study we have shown that UPR induction increases ERAD efficiency. Thus the UPR and ERAD are intimately coordinated and interdependent.
The identification of SON1 (PER8) as a gene required for ERAD provided an unexpected twist to the outcome of our genetic screen. SON1 (also called RPN4) was recently shown to be a transcriptional activator that binds a consensus promoter element, termed PACE (Mannhaupt et al. 1999). The PACE motif is found in many genes, but is a common element in the family of genes encoding subunits of the 26S proteasome. In son1 null mutants, the degradation of some cytosolic proteins are impaired at a step following ubiquitin conjugation (Johnson et al. 1995). Together, these data suggest SON1 regulates aspects of proteasome biogenesis, which in turn is required for ERAD. Surprisingly however, neither SON1 nor proteasomal subunit genes are regulated by the UPR (Travers et al. 2000). Rather, proteasomal subunit genes are coordinately upregulated in UPR-deficient cells following treatment with tunicamycin or DTT (Travers et al. 2000). These results suggest that, in addition to the UPR, a UPR-independent ER 
nucleus signal transduction pathway exists in which Son1p may play an important role. Interestingly, the UPR target genes PDI1 and PER5/RFT1 contain consensus PACE promoter elements (Mannhaupt et al. 1999), and may hence be regulated by Son1p, in addition to Hac1p.
Regulation of Protein Translocation Into and Out of the ER
The observation that UPR-deficient cells expressing CPY*HA exhibit general protein translocation defects revealed another unexpected role for the UPR. As discussed above, our analyses show a delay in the degradation of CPY*HA via ERAD. We consider it likely that these two phenomena are linked as several components of the ER translocon are thought to be both required protein import and export (Wiertz et al. 1996; Pilon et al. 1997; Plemper et al. 1997): UPR-regulated factors shared between import and export may become limiting under conditions as an extra load of an ERAD substrate needs to be attended to.
We confirmed this notion by testing two UPR target genes with known roles in translocation for import and export by increasing their gene dosage. We found that an extra copy of SEC61 was sufficient to alleviate the import defect, but degradation was still compromised (Fig. 7), while an extra copy of KAR2 restored both import and ERAD functions. These results suggest that primarily Kar2p becomes limiting under these conditions. Interestingly, expression of the mouse major histocompatability complex class I heavy chain H-2Kb in yeast also displayed rapid ER degradation that is dependent on UPR function (Casagrande et al. 2000). In apparent contrast to our results, Kar2p overexpression showed no improvement of heavy chain degradation in ire1 cells. We also observed, however, that more dramatic overexpression of Kar2p can actually inhibit the degradation of CPY*HA in wild-type cells (Spear, E.D., and D.T.W. Ng, unpublished results; J. Brodsky, personal communication). Taken together, these data suggest that a precise and balanced modulation of UPR targets is required for optimal ERAD activity.
These results serve to illustrate a simple approach that possibly can be more broadly exploited to study pathways under UPR control. Starting with a UPR-deficient strain, one imposes a specific nonlethal stress that in wild-type cells would elicit a moderate UPR induction (in this case, moderate CPY*HA expression). With the UPR off, key components become limiting. As defects are detected, it becomes possible to test the contribution of specific UPR target genes by increasing their gene dosage. Only some of many genes required for any given function are regulated by the UPR (Travers et al. 2000) which, we surmise, are likely to comprise those components that are rate limiting under uninduced conditions. Thus, the approach may allow to identify key regulatory components even for processes that require the contribution of multiple genes.
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Acknowledgments |
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This work was supported by grants from the National Institutes of Health to P. Walter and D.T.W. Ng. P. Walter is an Investigator of the Howard Hughes Medical Institute.
Submitted: 21 April 2000
Revised: 31 May 2000
Accepted: 31 May 2000
Abbreviations used in this paper: CPY, carboxypeptidase Y; ERAD, ER-associated protein degradation; GPI, glycosylphosphatidylinositol; PER, protein processing in the ER gene; UPR, unfolded protein response; UPRE, unfolded protein response element.
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