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© The Rockefeller University Press,
0021-9525/2000//1177 $5.00
The Journal of Cell Biology, Volume 150, Number 5,
, 2000 1177-1188
Original Article |
Requirement for Matrix Metalloproteinase Stromelysin-3 in Cell Migration and Apoptosis during Tissue Remodeling in Xenopus laevis
shi{at}helix.nih.gov
The matrix metalloproteinase (MMP) stromelysin-3 (ST3) was originally discovered as a gene whose expression was associated with human breast cancer carcinomas and with apoptosis during organogenesis and tissue remodeling. It has been shown previously, in our studies as well as those by others, that ST3 mRNA is highly upregulated during apoptotic tissue remodeling during Xenopus laevis metamorphosis. Using a function-blocking antibody against the catalytic domain of Xenopus ST3, we demonstrate here that ST3 protein is specifically expressed in the cells adjacent to the remodeling extracellular matrix (ECM) that lies beneath the apoptotic larval intestinal epithelium in X. laevis in vivo, and during thyroid hormone–induced intestinal remodeling in organ cultures. More importantly, addition of this antibody, but not the preimmune antiserum or unrelated antibodies, to the medium of intestinal organ cultures leads to an inhibition of thyroid hormone–induced ECM remodeling, apoptosis of the larval epithelium, and the invasion of the adult intestinal primodia into the connective tissue, a process critical for adult epithelial morphogenesis. On the other hand, the antibody has little effect on adult epithelial cell proliferation. Furthermore, a known MMP inhibitor can also inhibit epithelial transformation in vitro. These results indicate that ST3 is required for cell fate determination and cell migration during morphogenesis, most likely through ECM remodeling.
Key Words: apoptosis • matrix metalloproteinase • Xenopus laevis • thyroid hormone • metamorphosis
© 2000 The Rockefeller University Press
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Introduction |
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The ECM is made of numerous proteinaceous and nonproteinaceous components (Hay 1991; Timpl and Brown 1996). Its remodeling is accomplished largely through the action of matrix metalloproteinases (MMPs). MMPs are a family of Zn2+-dependent extracellular proteases (Alexander and Werb 1991; Woessner 1991; Birkedal-Hansen et al. 1993; Parks and Mecham 1998). They are synthesized as preenzymes and secreted extracellularly as inactive proenzymes, with the exception of membrane-type MMPs and stromelysin-3 (ST3), which are matured to active enzymes intracellularly and are inserted into the plasma membrane and secreted extracellularly, respectively (Pei and Weiss 1995; Uria and Werb 1998). The proenzymes are kept inactive through the coordination of the catalytic Zn2+ ion by the cysteine residue conserved in the propeptide and are activated upon the removal of the propeptide through various mechanisms (Van Wart and Birkedal-Hansen 1990; Nagase et al. 1992; Kleiner and Stetler-Stevenson 1993; Murphy et al. 1994; Nagase 1998).
The activated MMPs are then capable of cleaving proteinaceous components of the ECM with distinct but overlapping substrate specificities (Sang and Douglas 1996; Uria and Werb 1998). Thus, differential regulation and activation of different MMPs can lead to distinct ECM remodeling to facilitate various developmental and pathological processes. In fact, several MMP genes have been found to be highly expressed during tumor invasion and tissue remodeling in processes such as wound healing (Tryggvason et al. 1987; Alexander and Werb 1991; Matrisian 1992; Birkedal-Hansen et al. 1993; Stetler-Stevenson et al. 1993). Of particular interest among the MMPs is ST3, which was first identified as a gene associated with human breast cancer carcinomas (Basset et al. 1990). Furthermore, ST3 mRNA is also highly expressed in mammalian tissues where cell death takes place during development (Basset et al. 1990; Lefebvre et al. 1992; Muller et al. 1993). On the other hand, direct proof for the role of ST3 in apoptosis and cell migration has been lacking.
Amphibian metamorphosis is an excellent model to study the developmental functions of MMPs. Over 30 years ago, Gross and Lapiere 1962 discovered the first MMP, collagenase, in the resorbing tadpole tail. More recently, we and others have shown that ST3 gene is upregulated during Xenopus laevis metamorphosis (Wang and Brown 1993; Patterton et al. 1995), a process that is controlled by thyroid hormone (TH) (Dodd and Dodd 1976; Gilbert and Frieden 1981). Furthermore, its mRNA is temporally and quantitatively correlated with apoptosis in various organs and tissues (Patterton et al. 1995; Ishizuya-Oka et al. 1996; Berry et al. 1998a,Berry et al. 1998b; Damjanovski et al. 1999). We demonstrate here that ST3 protein is spatially and temporally associated with not only apoptosis but also the remodeling of the basal lamina underlying the degenerating larval epithelium during both natural and TH-induced intestinal metamorphosis. Furthermore, we provide direct evidence for a role of ST3 in facilitating larval epithelial cell death and adult epithelial morphogenesis during intestinal metamorphosis.
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Materials and Methods |
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A cDNA fragment encoding the catalytic domain (ST3-N, amino acids 86–247; Patterton et al. 1995) or the carboxyl hemopexin domain (ST3-C, amino acids 247–477; Patterton et al. 1995) was cloned into the overexpression vector pET-28a (Novagen) that contains a T7 RNA polymerase promoter. A cDNA fragment encoding the full-length ST3 was also cloned into a pSP64-based vector that contains a SP6 RNA polymerase promoter. The respective proteins were generated by using the TNT Quick Coupled Transcription/Translation System (Promega) in vitro in the presence of [35S]methionine. Equal amounts of the reaction mixture were electrophoresed on triplicate SDS-protein gels. One gel was dried and autoradiographed to detect the [35S]methionine-labeled in vitro–translated proteins, the other two were subjected to Western blot analysis with a pAb against the catalytic domain of ST3 or the preimmune serum as described in Ishizuya-Oka et al. 1997. For the analysis of ST3 during intestinal development, tadpole small intestine was extracted with the lysis buffer (2% SDS, 1 mM EDTA, 50 mM Tris-HCl, pH 6.8) and 10 µg/lane of the resulting protein was subjected to Western blot analysis by using the pAb.
2-Macroglobulin Capture Assay
[35S]Methionine-labeled ST3-N was generated through in vitro translation as above and used in the 2-macroglobulin (2M) capture assay as described (Reddy et al. 1994; Pei et al. 1994). 1 µl of the in vitro translation product was mixed with 5 µl of the reaction buffer (150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 0.1 mM ZnCl2, 20 mM Tris-HCl, pH 6.8) and 2 µl PBS containing the indicated amount of anti-ST3 or preimmune serum to reach the final concentration of 0–1%. The mixture was incubated for 30 min at room temperature before the addition of 2 µl (1 µg) of 2M and incubation for another 60 min. The resulting mixture was then analyzed on a native polyacrylamide gel (4/20% gradient gel). The gel was stained with Coomassie blue to identify the position of the 2M and then dried and autoradiographed to identify the 2M–ST3 complex. It should be pointed out that preincubation of ST3 with the serum was not important, as the anti-ST3 antibody also blocked the complex formation between ST3 and endogenous serum 2M (see Results).
1-Antitrypsin Cleavage
ST3 catalytic domain (ST3-N) cloned in pET-28a (alone) was overexpressed in Escherichia coli and purified using the His-tag at the NH2 terminus on a Ni-NTA spin column according to the manufacturer's instructions (QIAGEN). The ST3 was eluted into 0.5 M NaCl, 10 mM CaCl2, 200 mM imidazole, 0.1% Triton X-100, 20 mM Tris-HCl, pH 7.9. The purified ST3 (1 µl of 40 ng/µl) was incubated overnight with 1.5 µg of human 1-antitrypsin (Athens Research and Technology, Inc.) in 20 µl of 200 mm NaCl, 10 mM CaCl2, 0.1% Triton X-100, 50 mM Tris-HCl, pH 7.5, in the presence or absence of the indicated amount of preimmune or anti-ST3 antibody. The samples were than subjected to Western blot analysis with anti–human 1-antitrypsin antibody (Calbiochem).
Tissue Preparation
Tadpoles of the South African clawed frog (X. laevis) from stage 55 (
4 wk after fertilization) to 66 (2 mo after fertilization and the end of metamorphosis; Nieuwkoop and Faber, 1956) were purchased from a commercial source in Hamamatsu, Japan. Tissue fragments were isolated from the anterior part just behind the bile duct junction of the small intestine, unless otherwise noted.
Organ Culture
Tissue fragments isolated from tadpoles at stage 57 (before metamorphic climax) were cultured as described in our previous paper (Ishizuya-Oka and Shimozawa 1991). In brief, the tubular fragments were slit open lengthwise with forceps and cultured in the medium containing 60% Leibovitz-15 medium supplemented with 100 IU/ml of penicillin, 100 µg/ml of streptomycin, and 10% fetal bovine serum, which had been treated with activated charcoal (Sigma-Aldrich) according to the method of Yoshizato et al. 1980 to remove endogenous thyroid hormones (CTS medium). To induce metamorphosis, 3,3',5-triiodo-L-thyronine (T3), insulin, and hydrocortisone (Sigma-Aldrich) were added to CTS medium at 10–8 M, 5 µg/ml, and 0.5 µg/ml, respectively (TH-containing medium). Where indicated, the general inhibitor of MMPs, 4-Abz-Gly-Pro-D-Leu-D-Ala-NH-OH (MMP inhibitor I; Calbiochem) was added at 100 µg/ml (Odake et al. 1994). In addition, to examine the effects of the anti–Xenopus ST3 antiserum on the intestinal remodeling, the antiserum was added to TH-containing medium at various concentrations. In our preliminary experiment, when high concentrations of the antiserum (5% and higher) were added to the medium, necrosis of the epithelial cells was often recognized before 3 d of cultivation (not shown). Therefore, in this study, we used antiserum at concentrations of 0–1%, where the necrosis was undetectable at least until 3 d of cultivation. As a control, the same concentration of preimmune rabbit serum was added to the medium instead of the anti-ST3 antiserum. The culture medium was changed every other day for the 5-d treatment at 26°C.
Electron Microscopy
The explants were fixed in 7.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) at 4°C for 2 h, postfixed with 1% osmium tetroxide in the same buffer at 4° for 2 h, stained en bloc with uranyl acetate, and embedded in epoxy resin. Ultrathin sections were stained with lead citrate and examined with a JOEL 200CX electron microscope.
Immunohistochemistry for ST3
Tissue fragments and cultured explants were fixed with 95% ethanol, processed for paraffin embedding, and cut at 5 µm. The sections were incubated for 1 h with the polyclonal antiserum (diluted 1:500) and then visualized by sequential incubation with streptavidin-biotin–peroxidase complex and DAB/H2O2, as described above. There was no positive staining when preimmune rabbit serum was applied (diluted 1:500) as the specificity control (not shown). At least three fragments were examined for each experimental point.
Nick End Labeling of Apoptotic DNA
DNA fragmentation associated with apoptosis was detected according to the terminal deoxyribonucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labelling (TUNEL) method of Gavrieli et al. 1992. In brief, tissue fragments were fixed in 4% paraformaldehyde at 4°C for 4 h, and embedded in paraffin. Sections cut at 5 µm were treated with 20 µg/ml proteinase K (Boehringer) at room temperature for 15 min, and immersed in 1% H2O2 at room temperature for 15 min to inactivate endogenous peroxidase. They were then incubated with TdT buffer (100 mM potassium cacodylate, pH 7.2, 2 mM cobalt chloride, 0.2 mM DTT; GIBCO BRL) containing 0.3 equivalent U/µl terminal deoxynucleotidyl transferase (TdT; GIBCO BRL) and 0.04 mM biotinylated-dUTP (Boehringer) at 37°C for 90 min. The reaction was terminated by placing the slides in a washing solution (300 mM sodium chloride and 30 mM sodium citrate) for 15 min at room temperature. After the treatment with 10% normal rabbit serum for 10 min, the sections were incubated with peroxidase-labeled streptavidin (Nichirei) for 30 min and then with 0.02% 3,3'-diaminobenzidine-4HCl (DAB) and 0.006% H2O2 for 5 min. As a positive control, some sections were pretreated with 2 U/µl DNase I (Sigma-Aldrich) in 30 mM Tris buffer, pH 7.4, for 10 min before the treatment with TdT. The omission of either TdT or dUTP gave completely negative results (not shown). The labeling indices were calculated as the ratio of clearly labeled intact nuclei to total epithelial nuclei in >10 sections randomly selected from each explant cultured for 3 d. More than three explants were examined for each experimental point. Results were statistically analyzed by Student's t test.
Staining with Methyl Green–Pyronin Y
To identify adult epithelial primordia, explants cultured for 5 d were stained with methyl green–pyronin Y (Muto) as described by Ishizuya-Oka and Ueda 1996. During the replacement of larval epithelial cells by adult ones, the adult epithelial cells were intensely stained red because of their RNA-rich cytoplasm, whereas the staining of larval epithelial cells became much weaker towards their death (Ishizuya-Oka et al. 1997).
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Results |
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Concurrent Induction of ST3 Expression by TH with Intestinal Remodeling in Organ Cultures
Like other processes during amphibian metamorphosis, intestinal remodeling is under the control of TH. Furthermore, this process is organ autonomous and can be induced by simply adding physiological concentrations of TH to the culture medium of intestinal fragments from premetamorphic tadpoles (Ishizuya-Oka and Shimozawa 1991). Immunohistochemical analysis showed that ST3 was absent in such intestinal explants from premetamorphic tadpoles cultured in the absence of TH (Fig. 4 E) or at the onset of TH treatment (Fig. 4 A). After 3 d of treatment, ST3 was expressed in the fibroblastic cells underneath the larval epithelium that had begun the degeneration process as reflected by the presence of lysosome-like granules (Fig. 4 B). By the fifth day of treatment, as larval epithelial degeneration approached completion, ST3 could only be detected weakly in a few cells in the connective tissue as epithelial cell death approached completion (Fig. 4 C). Similar to observations in vivo, high levels of TH-induced ST3 expression were accompanied by the increased folding of the basal lamina (Fig. 4 F), whereas in the absence of TH, the basal lamina remained as a continuous thin lining separating the epithelium and connective tissue (Fig. 4 G).
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2M. Thus, in vitro–synthesized 35S-labeled ST3 catalytic domain was incubated with 2M in the absence or presence of anti-ST3 antiserum or preimmune serum. The resulting mixture was electrophoresed on a native polyacrylamide gel. Under the gel conditions, free ST3 failed to enter the gel due to the lack of negative charges associated with SDS binding under denaturing conditions, whereas 2M–ST3 complex migrated at the same position as free 2M as they were similar in size (Fig. 6 A, compare lanes 2 and 11; the bottom panel shows the staining of 2M by Coomassie blue). The addition of anti-ST3 antiserum led to a dose-dependent inhibition of the formation of the 2M–ST3 complex and a concurrent formation of ST3–antibody complex (Fig. 6 A, bottom band in lanes 3–6; note that this antibody complex formed regardless of whether 2M was present, but was absent when preimmune serum was used, lanes 7–10). In contrast, the preimmune serum had no effect on 2M–ST3 complex formation. In fact, ST3 also formed a complex with endogenous 2M in the preimmune serum (Fig. 6 A, lane 10 where no exogenous 2M was added; the endogenous 2M migrated slightly slower than exogenous 2M, compare lanes 9 and 10), which was also inhibited by the anti-ST3 antibody (compare lanes 6 and 10).
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1-antitrypsin, a known substrate of mammalian ST3 (Pei et al. 1994) in the presence or absence of the antibody. Similar to mammalian ST3, the frog ST3 specifically cleaved 1-antitrypsin to produce two fragments. The larger fragment, as well as the full-length 1-antitrypsin, could be detected by a specific antibody (Fig. 6 B, lanes 1 and 2). The addition of as little as 1% of anti-ST3 antiserum completely blocked the cleavage whereas as much as 10% of the preimmune serum had no effect (Fig. 6 B; a slight enhancement was observed, possibly due to the stabilization of ST3 by the serum). Together, these data indicate that the anti-ST3 antibody is a function-blocking antibody. To see if blocking ST3 function can influence intestinal remodeling, the anti-ST3 antiserum was added to the medium of intestinal organ cultures and its effect on TH-induced intestinal remodeling was analyzed. First, electron microscopic examination of the basal lamina showed that when the ST3 antiserum was included in the medium at a final concentration of 1% (the culture medium also contained 10% TH-depleted fetal bovine serum), it inhibited or delayed the remodeling of the ECM. After 3 d of TH treatment in the presence of the antibody, the basal lamina remained a thin, continuous layer (Fig. 4 H). This contrasts with the absence of the antibody but presence of TH (Fig. 4 F), although prolonged culturing eventually led to the remodeling of the basal lamina (data not shown). Thus, ST3 is directly or indirectly involved in ECM remodeling.
When cell death was analyzed with the TUNEL assay, it demonstrated that the apoptosis in the larval epithelium was also blocked by the antibody when assayed after 3 d of TH treatment (Fig. 7 A). In contrast, the preimmune serum had no effect on TH-induced apoptosis (Fig. 7 B). In addition, when an antibody generated against the His-tagged hemopexin domain of ST3, which failed to recognize the full-length ST3, was added to the culture medium, it had no effect on the TH-induced apoptosis. Finally, an antibody against another TH-induced gene, the Xenopus sonic hedgehog, also failed to influence TH-induced cell death (data not shown). These controls indicate that the effect of the anti-ST3 catalytic domain antibody was due to specific inhibition of ST3 function.
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5 d) led to the degeneration of larval epithelium even in the presence of the anti-ST3 serum (data not shown; see also Fig. 8).
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Involvement of ST3 in Adult Epithelial Cell Migration
The intestinal organ culture also reproduces the second major event of the metamorphic process, i.e., the development of the adult epithelium. Upon prolonged TH treatment in vitro, adult epithelial primordia generally developed as small islets. These new epithelial cells proliferate rapidly to replace the dying larval cells. During this process the two types of epithelial cells could be distinguished by the differences in the intensity of their staining with methyl green–pyronin Y; the adult epithelial primordia consisting of undifferentiated cells were strongly stained red, whereas the apoptotic larval cells appeared pale as observed during spontaneous metamorphosis (Fig. 8). During the first 2 d, the larval epithelial cells remained healthy and stained red strongly (Fig. 8 A). After 3 d of TH treatment, the staining became weak as the larval cells underwent apoptosis (Fig. 8 B). The adult epithelial primordia were not detectable until 5 d of culturing and they were variable in size. These cells were located just beneath the larval epithelium and invaded into the underlying connective tissue as they grew in size (Fig. 8 C), again, similar to what occurs during spontaneous metamorphosis.
As was the case for larval epithelial cell death, anti-ST3 antiserum was found to alter adult primordial development when added at 0.2% or higher concentrations. In particular, the vertical invasion of the adult epithelial primodia into the underlying connective tissue was blocked (Fig. 8 D). However, cell proliferation of the adult epithelial primodia appeared to continue in the presence of the antibody. The proliferating cells expanded only horizontally along the epithelial connective tissue interface but failed to protrude into the connective tissue. Again, as controls, preimmune serum had no effect on either cell proliferation or invasions (data not shown), and the explant without TH, but with the antiserum, remained unchanged throughout the cultivation (Fig. 8 E).
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ECM Remodeling Influences Intestinal Epithelial Cell Fate
Intestinal epithelial morphogenesis is one of the essential processes that occur during postembryonic development in vertebrates. In anurans, this process involves the degeneration of the larval epithelium and the development of the adult epithelium (Dauca and Hourdry 1985; Yoshizato 1989; Shi and Ishizuya-Oka 1996). The epithelium lies above the basal lamina, which is a special ECM that separates the epithelium from the connective tissue. During both natural and TH-induced metamorphosis, the basal lamina changes from a continuous but thin structure to an amorphous, discontinuous, but thick structure (Fig. 2 and Fig. 3). Furthermore, the connective tissue and epithelium play a mutually interactive role to facilitate each other's development (Ishizuya-Oka and Shimozawa 1992a,Ishizuya-Oka and Shimozawa 1992b, Ishizuya-Oka and Shimozawa 1994). In addition, cells directly interact with ECM; thus, any changes in ECM can affect cell behavior. In fact, studies using several cultured cell lines have shown that proper cell–ECM interactions are important for cell survival (Ruoslahti and Reed 1994; Boudreau et al. 1995; Frisch and Ruoslahti 1997; Shi et al. 1998). One of the best examples is the mammary gland epithelial cells, which undergo apoptosis when cultured on plastic dishes. Such programmed cell death can be inhibited by culturing the cells on an exogenous basement membrane. In addition, blocking cell–ECM interactions with specific anti-integrin antibodies induces apoptosis of this and various other types of cells (Boudreau et al. 1995; Frisch and Ruoslahti 1997). Thus, it is tempting to suggest that the remodeling of the basal lamina during metamorphosis plays a critical role in the removal of the larval epithelium and development of the adult epithelium.
Direct evidence to support a role of ECM in intestinal development has come from studies using primary cell cultures. By culturing epithelial or fibroblastic cells from prometamorphic tadpole intestine, Su et al. 1997 have shown that TH can induce the proliferation of both cell types and concurrently cause the epithelial, but not fibroblastic, cells to undergo apoptosis, similar to what occurs during intestinal metamorphosis. Interestingly, adult epithelial cells from metamorphosing intestine also undergo apoptosis when treated with TH in cell cultures (Su et al. 1997), suggesting that removing the ECM support makes the cells vulnerable to TH-induced death. More importantly, larval epithelial cells cultured on various ECM-coated dishes are more resistant to TH-induced death (Su et al. 1997), supporting a key role of ECM in cell fate determination.
Dual Functions of ST3 during Intestinal Development
We have demonstrated here for the first time that the expression of ST3 proteins is spatially and temporally correlated with larval cell death and adult epithelial morphogenesis, in agreement with mRNA data reported previously (Wang and Brown 1993; Patterton et al. 1995, Ishizuya-Oka et al. 1996, Damjanovski et al. 1999). Although it was difficult to detect ST3 protein in organ cultures after 1–2 d of TH treatment, due to low sensitivity of immunohistochemistry, ST3 has been shown to be a direct TH response, whose mRNA is induced by TH within 4–8 h (Wang and Brown 1993; Patterton et al. 1995). Thus, ST3 protein is most likely present before larval epithelial apoptosis and could influence this process. Indeed, our results here indicate that ST3 has dual roles during intestinal metamorphosis. It is important not only for larval epithelial cell death, but also for the invasion of the adult epithelial primordia into the underlying connective tissue, a process that is critical for the morphogenesis of the adult epithelium, such as in epithelial fold formation.
It is worth noting that ST3 is the only MMP shown to be tightly correlated with larval cell death and adult epithelial morphogenesis. In situ hybridization and morphological examination indicate that ST3 is expressed in the fibroblastic cells of the connective tissue, although a definitive determination of the cell type(s) remains to be done (Patterton et al. 1995; Damjanovski et al. 1999). This suggests that as an MMP, ST3 may affect epithelial development by either directly or indirectly modifying the ECM. Indeed, ST3 expression also correlates with the extensive ECM remodeling during metamorphosis, and blocking ST3 expression inhibits ECM remodeling in vitro (Fig. 4 H; Patterton et al. 1995; Brown et al. 1996; Stolow et al. 1996; Damjanovski et al. 1999). In this paper, we present the first direct functional data to support a physiological role of ST3 during intestinal remodeling. Although ST3 is expressed in the connective tissue, it is required for epithelial transformation. A similar requirement of factors from neighboring tissues has also been observed for tail resorption in Rana catesbeiana (Niki et al. 1982; Niki and Yoshizato 1986), where an epidermal factor was found to be important for mesenchymal regression, although the factor has yet to be identified.
It is worth noting that while ECM remodeling correlates with the ST3 expression, the basal lamina is actually thicker but multiply folded at the peak of ST3 expression. This suggests that ST3 causes, either directly or indirectly, cleavage of certain substrate(s). The apparent increase in the basal lamina may be due to new ECM synthesis and/or the intestinal contraction as the intestine reduces its length during metamorphosis (Shi and Ishizuya-Oka 1996). Regardless, the result is consistent with the idea that ECM remodeling, not necessarily its degradation, plays a critical role in cell fate determination.
ST3 was initially discovered as an MMP associated with human breast cancer carcinomas and with apoptotic organogenesis (Basset et al. 1990; Lefebvre et al. 1992, Lefebvre et al. 1995; Muller et al. 1993). However, direct proof for a role of ST3 in cell migration and cell fate determination had been elusive due to the difficulties of working with this enzyme. Recent gene knockout studies have demonstrated that mice lacking ST3 had reduced incidence of carcinogen-induced tumors, whereas overexpression of ST3 in cultured cells led to increased tumor incidence when the resulting cells were injected into mice (Noel et al. 1996; Masson et al. 1998). Our study provides direct evidence that ST3 is indeed important for both cell migration and cell death. For proliferating epithelial cells of the metamorphosing intestine, ST3 facilitates migration/invasion, whereas for terminally differentiated larval epithelial cells, it participates in promoting apoptosis. Such differential effects are in agreement with the observed expression profiles of ST3 in both mammals and Xenopus (Basset et al. 1990; Lefebvre et al. 1995; Patterton et al. 1995; Brown et al. 1996; Muller et al. 1993).
Interestingly, blocking the function of ST3 was unable to completely block the TH-induced cell death, as prolonged TH treatment eventually caused larval epithelium to degenerate (Fig. 7), suggesting that the role of ECM remodeling lies mainly at facilitating or coordinating tissue transformations but is not the determining factor for cell fate, or that the inhibition was incomplete. Such an effect also bears a similarity to the observation that homologous deletion of gelatinase B resulted in a delay in terminal hypertrophic chondrocyte apoptosis during mouse development (Vu et al. 1998).
Finally, our findings also complement recent studies with transgenic mice overexpressing another MMP, stromelysin-1, which showed that overexpression of the MMP leads to ECM remodeling and cell death (Sympson et al. 1994; Witty et al. 1995a,Witty et al. 1995b).
Like these and other studies such as the recent gene knockout studies on gelatinase B (Vu et al. 1998) and MT1-MMP (Holmbeck et al. 1999), it is unknown how MMPs exert their effects. They can either directly or indirectly modify the ECM to influence cell death and/or cell migration. Our results are consistent with this possibility. As discussed above, ECM remodeling can lead to apoptosis. Likewise, MMPs have long been implicated to play a role in tumor invasion. We have shown that ST3 is also important for cell migration during development. The increase in basal lamina thickness at the peak of ST3 expression suggests that selective cleavages of the ECM proteins, not massive ECM degradation, is sufficient for such an effect. In this regard, it is worth noting that specific cleavage of the ECM protein laminin-5 by MMP-2 or gelatinase A can induce cell migration (Giannelli et al. 1997). Thus, it is likely that alterations in cell–ECM interaction, not the mere loss of cell–ECM contacts, activate cell migration processes.
Alternatively, MMPs can function through other pathways. In the case of ST3, this was made possible by the discovery of the ability of this MMP to cleave a serpin, which inhibits serine proteases (Pei et al. 1994). Several studies have shown that serine proteases can influence cell behavior, including cell differentiation, growth, and apoptosis (Turgeon and Houenou 1997). For example, the serine protease thrombin can alter neurite outgrowth and induce neuronal cell death (Turgeon et al. 1998), whereas the serpin protease nexin I inhibits neuronal apoptosis (Houenou et al. 1995). Although it remains to be shown that ST3 cleaves serpins in vivo, these findings raise the possibility that ST3 may facilitate cell death by enhancing the activities of serine proteases through the removal of their inhibitors. Likewise, ST3 may cleave other non-ECM substrates to regulate cell behavior. Thus, the future challenges will be (a) to determine whether ST3 functions by directly or indirectly modifying the ECM, or through other pathways; (b) to identify the in vivo substrates of ST3; and (c) to determine the mechanisms underlying the effects of ST3 on cell death and migration.
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Acknowledgments |
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Submitted: 17 March 2000
Revised: 12 July 2000
Accepted: 13 July 2000
Q. Li, T. Amano, and S. Damjanovski contributed equally to this work.
2M, 2-macroglobulin; ECM, extracellular matrix; MMP, matrix metalloproteinase; ST3, stromelysin-3; TdT, terminal deoxyribonucleotidyl transferase; TH, thyroid hormone; TUNEL, TdT-mediated dUTP-biotin nick end labeling.
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) of TH were measured daily. Each value represented the mean ± SD. Bars, 20 µm.
