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© The Rockefeller University Press,
0021-9525/2000//673 $5.00
The Journal of Cell Biology, Volume 151, Number 3,
, 2000 673-684
Original Article |
The Dendritic Cell Receptor for Endocytosis, Dec-205, Can Recycle and Enhance Antigen Presentation via Major Histocompatibility Complex Class II–Positive Lysosomal Compartments
Many receptors for endocytosis recycle into and out of cells through early endosomes. We now find in dendritic cells that the DEC-205 multilectin receptor targets late endosomes or lysosomes rich in major histocompatibility complex class II (MHC II) products, whereas the homologous macrophage mannose receptor (MMR), as expected, is found in more peripheral endosomes. To analyze this finding, the cytosolic tails of DEC-205 and MMR were fused to the external domain of the CD16 Fc
receptor and studied in stable L cell transfectants. The two cytosolic domains each mediated rapid uptake of human immunoglobulin (Ig)G followed by recycling of intact CD16 to the cell surface. However, the DEC-205 tail recycled the CD16 through MHC II–positive late endosomal/lysosomal vacuoles and also mediated a 100-fold increase in antigen presentation. The mechanism of late endosomal targeting, which occurred in the absence of human IgG, involved two functional regions: a membrane-proximal region with a coated pit sequence for uptake, and a distal region with an EDE triad for the unusual deeper targeting. Therefore, the DEC-205 cytosolic domain mediates a new pathway of receptor-mediated endocytosis that entails efficient recycling through late endosomes and a greatly enhanced efficiency of antigen presentation to CD4+ T cells.
Key Words: dendritic cell • antigen presentation • DEC-205 • MHC class II • endocytosis
© 2000 The Rockefeller University Press
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Introduction |
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Adsorptive endocytosis receptors, like the macrophage mannose receptor (MMR), FcR, and B cell antigen receptor (BCR), are used in the immune system to facilitate antigen capture and presentation of peptides to T cells (Bonnerot et al. 1992; Sallusto et al. 1995; Engering et al. 1997). Previously we have identified an endocytic receptor, termed DEC-205, expressed by dendritic cells (DCs). This 205-kD protein contains 10 external, contiguous, C-type lectin domains and by sequence analysis, is a homologue of the MMR. In fact, both the MMR and DEC-205 mediate adsorptive uptake, and both have cytosolic domains with requisite coated pit localization sequences (Stahl et al. 1980; Jiang et al. 1995). Therefore, we expected that both MMR and DEC-205 would recycle through early endosomal compartments and present bound antigens comparably. However, we will show that DEC-205 unexpectedly targets to late endosomes or lysosomes in developing DCs, and that DEC-205 is far superior to the MMR in presenting bound rabbit antireceptor antibodies to T cells, a classical assay for measuring the presenting function of endocytosis receptors (Chesnut and Grey 1981).
To dissect the role of the cytosolic domain in these findings, we studied a totally heterologous system: L cells stably transfected with a fusion receptor formed between the external domains of human CD16 and different cytosolic tails. We find that the DEC-205 cytosolic domain has a distinct distal region with an acidic EDE triad. The distal region and its acidic sequence are required for recycling beyond early endosomes through deeper major histocompatibility complex class II–positive (MHC II+), late endosomes and lysosomes. Such distal targeting is unique for adsorptive endocytosis receptors that have been analyzed to date, and proves to be necessary for presentation of antigenic peptides at low doses of ligand. This new pathway for receptor-mediated uptake is therefore a hybrid of the two known endocytic pathways discussed above, targeting deeper digestive compartments but recycling efficiently to produce biologically active peptides.
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Materials and Methods |
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Cloning and Transfection
The cDNAs coding for the intracellular domains of DEC-205 and MMR were cloned separately from the extracellular expressed domains into a TA vector (Invitrogen), and oligonucleotide directed mutagenesis using QuikChange® (Stratagene) was performed. STOP codons and a tyrosine to alanine exchange were introduced into different parts of the DEC tail (see Fig. 1 A). All mutations were verified by sequencing (DNA Technology Center, The Rockefeller University). Using standard methods, the mutated DEC tails were cloned into the BamH1-EcoR1 site of the pApuro vector (gift of Dr. Kurosaki, American Cynamid Company, Lederle Laboratories, Department of Cardiovascular Molecular Biology, Pearl River, NY) behind the sequence coding for the extracellular domain of the human FcIII receptor (Kolanus et al. 1993). After verifying the correct insertion by sequencing, these constructs were transfected into the MHC II+ (I-Ek) fibroblast cell line DCEK.ICAM.Hi7 by calcium phosphate precipitation (Stratagene). Thereafter, cells were split into 100-mm dishes and for selection, puromycin (Calbiochem) was applied at a final concentration of 5 µg/ml. Colonies growing under selection after 2–3 wk were picked and expanded. Surface expression of CD16 was regularly monitored by FACS®, using anti-CD16 antibody (clone 3G8).
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Surface Binding and Internalization of Human IgG
Human IgG (HuIgG; Jackson ImmunoResearch Laboratories) was diluted to 1 mg/ml in PBS. Aggregates were formed by incubation at 65°C for 30 min. Thereafter, aliquots were stored at 4°C until use, but no longer than 1 wk. In some antigen-presenting assays, HuIgG was aggregated by incubation with goat anti-HuIgG antibodies for 4 h at room temperature. To assay surface binding of HuIgG, cells were harvested and washed with cold PBS/1% FCS (vol/vol) and incubated for 45 min on ice with monomeric or aggregated HuIgG (aggHuIgG) at 0.1–100 µg/ml. After washes with cold PBS/1% FCS (vol/vol), FITC-labeled goat anti–human IgG antibodies (Jackson ImmunoResearch Laboratories) at a final concentration of 1 µg/ml in PBS/1% FCS (vol/vol) were added. After 45 min on ice, cells were washed, resuspended in PBS, and analyzed on a FACScanTM. To measure internalized HuIgG, the DCEK.ICAM.Hi7 cells were seeded into 96-well plates (20,000 cells/well), cultured overnight, and transferred on ice followed by addition of HuIgG at a final concentration of 50 µg/ml. After 45 min, unbound HuIgG was removed by washing with medium, and aliquots of cells were harvested and fixed with 4% paraformaldehyde (PFA). The remaining cells were further incubated in R7 at 37°C after which the cells were harvested, fixed, and stained with FITC-labeled goat anti–human IgG antibodies as described above. To detect internalized HuIgG, cells were permeabilized before adding FITC-labeled goat anti–human antibodies, using a 10-min incubation with PBS/0.1% (vol/wt) saponin (Sigma-Aldrich). In time course experiments, the amount of internalized HuIgG was calculated by subtracting the mean fluorescence in fixed cells (surface-bound HuIgG) from that recorded with fixed and permeabilized cells (internalized and surface-bound HuIgG).
Receptor Recycling
Cells were cultured for 2 h in 10 µg/ml cycloheximide (CHX; Calbiochem), sufficient to block protein biosynthesis as assessed by [35S]methionine labeling (not shown). Surface CD16 then was saturated by incubating the cells in presence of CXH with aggHuIgG (100 µg/ml) at 37°C for 10 min, followed by chilling the cells for 1 h on ice. Thereafter, cells were cultivated in R7 supplemented with CXH, and at different times, recycled CD16 receptors were detected by incubation with 125I-HuIgG at 10 µg/ml on ice. Bound HuIgG was measured by counting (Wallac).
Confocal Immunofluorescence Microscopy of L Cells and DCs
L cells were seeded into LabTek tissue culture chambers (Nunc) and incubated overnight. Cells were washed twice with warm RPMI medium, fixed with 4% paraformaldehyde/PBS (wt/vol) for 20 min at room temperature, and permeabilized by incubation with permeabilization buffer (RPMI containing 10% normal goat serum [GIBCO BRL], 0.05% saponin [Sigma-Aldrich], 10 mM glycine [Sigma-Aldrich]) for 15 min at room temperature. Alternatively, DCs were grown from bone marrow precursors using GM-CSF as described (Inaba et al. 1992). At day 6, when the cultures contain numerous aggregates of immature DCs with abundant MHC II compartments (MIICs) (Pierre et al. 1997), the cells were dislodged and applied to alcian blue–coated, glass slides for fixation as above. After two washes in permeabilization buffer, cells were incubated for 45 min at room temperature with the following antibodies: anti–MHC II (M5/114; American Type Culture Collection), anti–lysosome-associated membrane protein 1 (LAMP-1) (clone ID-4B; gift of Dr. Ira Mellman, Yale University School of Medicine, New Haven, CT), anti-transferrin receptor (TfR) (C2F2; American Type Culture Collection), anti-CD16 (clone 3G8), and either monoclonal or polyclonal antibody to DEC-205 (Swiggard et al. 1995) and MMR (prepared in rabbits by immunization with cloned MMR external domains). In double labeling experiments, FITC-conjugated MHC II and LAMP-1 antibodies (BD PharMingen) were used. For unconjugated antibodies, we stained cells for 45 min in permeabilization buffer with appropriate FITC- or Texas red–labeled secondary reagents, absorbed against mouse or rat proteins and applied at a final concentration of 1 µg/ml. For detection of endocytosed HuIgG, FITC-labeled goat anti-HuIgG was applied at 1 µg/ml in permeabilization buffer. Slides were mounted in aquamount (Polysciences) and examined by confocal laser scan microscopy (ZEISS). ZEISS software was used to take and to overlay pictures. Composite figures were made using Photoshop® (Adobe Systems).
Antigen Presentation to T Cells
To obtain HuIgG- or RbIgG-specific T cells, 6–8-wk-old B10.BR mice (The Jackson Laboratory) were primed to HuIgG or RbIgG by subcutaneous injection of 50 µg HuIgG or RbIgG emulsified in complete Freund's adjuvant (Difco). In some experiments, mice were primed to RbIgG using anti–DEC-205 Rb antiserum or anti-MMR Rb antiserum. 8 d later, draining lymph nodes were removed and single cell suspensions were prepared by teasing with forceps and forcing the nodes through a nylon mesh. T cells were purified by passage over nylon wool columns and incubating the eluted cells with antibodies directed against MHC II (M5/114; American Type Culture Collection) and CD45 (B220; American Type Culture Collection) on ice for 30 min. In some experiments, anti-CD8 antibodies (TIB 211; American Type Culture Collection) were added to obtain purified CD4+ T cells. Cells were washed and incubated with goat anti–rat Dynabeads (Dynal) at a ratio of 4 beads to 1 target cell for an additional 30 min at 4°C to remove non-T cells. For antigen presentation assays with DCEK.ICAM.Hi7 cells, transfected and untransfected DCEK.ICAM.Hi7 cells were irradiated with 5,000 rads and seeded into 96-well plates (15,000 cells/well). HuIgG was added to the cells in graded doses. After overnight culture, unbound HuIgG was removed by washing the plates with warm R7 medium. Thereafter, 250,000 T cells/well in 200 µl R7 were added in triplicate, and the plates were incubated for 3–4 d. For antigen presentation assays with DCs, developing bone marrow DCs were harvested after 6 d of culture in GM-CSF, placed on ice, and incubated with graded doses of rabbit anti–DEC-205, anti-MMR, and preimmune serum for 1 h. Unbound antibodies were washed away and aliquots of the DCs were seeded in triplicates into 96-well plates. Then 200,000 T cells/well were added and cultured for 3–4 d. To assay T cell proliferation, [3H]thymidine (1 µCi/well; Amersham Pharmacia Biotech) was added for the last 12 h before harvesting. Incorporation of radioactivity was determined by scintillation counting. Data are shown are means of triplicates where the standard deviation was <10% of the mean cpm.
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Results |
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By confocal laser scan microscopy, both the MMR and DEC-205 were abundant in intracellular granules (Fig. 1 A, red). As expected for a recycling endocytic receptor, very little of the MMR was found in late endosomes or lysosomes that were labeled for LAMP-1 or for MHC II (Fig. 1 A, green and merge images). In marked contrast, the bulk of the intracellular DEC-205 was localized in perinuclear MIICs, as demonstrated by colocalization with LAMP-1 and with MHC II (Fig. 1 A).
To detect a functional consequence for the distinct targeting of the MMR and DEC-205, we used the rabbit polyclonal antibodies as surrogate antigens for T cells primed to rabbit Ig, as Chesnut and Grey 1981 first did to show presentation via the BCR. When antibodies were added to immature DCs in the cold, the cells bound comparable amounts of anti-MMR and DEC-205 Ig (Fig. 1 B, top). When the same cells were added to cultures of primed T cells, the anti–DEC-205 was presented with much higher efficiency (Fig. 1 B, bottom). To rule out that the quantitative difference is due to different allotypic determinants within the MMR and DEC-205 antibodies, additional experiments were performed using T cells from mice primed to RbIgG with either anti–DEC-205 or anti-MMR antiserum (Fig. 1 C). Here again the anti–DEC-205 was presented with much higher efficiency regardless of whether the T cells were derived from animals primed with either DEC-205 or MMR antibodies. Thus, DEC-205 is normally found in MIICs, whereas MMR is predominantly found in early endosomes; when rabbit antibodies are bound to these two receptors, DEC-205 is much more efficient at presenting peptides to rabbit Ig–primed, CD4+ T cells.
Expression of Chimeric DEC-205/CD16 Receptors in Transfected DCEK.ICAM.Hi7 Cells
The targeting of endocytic receptors is determined by amino acid sequences within their intracellular domains (for review see Bonifacino and Dell'Angelica 1999). A reexamination of the cytosolic domains ("tails") of three homologous lectins—the MMR, DEC-205, and the phospholipase A2 receptor (PLA2R)—showed the tails of the MMR and PLA2R to be very similar to each other but different from DEC-205 (gray shading in Fig. 2 A). All three tails contained a membrane-proximal, putative coated pit localization sequence (Fig. 2 A, underlined) for uptake (Collawn et al. 1990), but the distal region of DEC-205 was distinct and included a sequence of three acidic amino acids (Fig. 2 A, EDE). Interestingly, acidic sequences in other receptors are implicated in intracellular sorting (Matter et al. 1993; Voorhees et al. 1995; Wan et al. 1998; Piguet et al. 1999; Simmen et al. 1999).
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III receptor or CD16 and different DEC-205 cytosolic tails (Fig. 2 B). In addition to the wild-type (WT) DEC-205 tail, we made truncations to remove the terminal three amino acids (long tail, LT), residues 19–31 with the putative EDE distal targeting sequence (intermediate tail, IT), and residues 6–31 with the coated pit sequence (short tail, ST). We also made mutants of the DEC-205 tail, converting tyrosine to alanine in the coated pit sequence (altered tail or AT), and EDE to alanines in the putative distal targeting sequence. We used the wild-type MMR tail for comparison. The CD16 chimeras were transfected into a murine fibroblast-like line, DCEK.ICAM.Hi7, which expresses MHC II as well as the T cell costimulatory molecules B7-1 (CD80) and ICAM-1 (CD54) (Dubey et al. 1995). Comparable surface expression of each CD16 chimeric receptor was obtained (Fig. 2 C) in the stable transfectants, without altering expression of MHC II (Fig. 2 C) or B7-1 (not shown).
Binding, Uptake, and Recycling of HuIgG by CD16 Chimeric Receptors
To test the function of the chimeric receptors, we first measured binding of HuIgG as illustrated for WT-DEC:CD16 (Fig. 3 A). The transfectants all bound ligand. Saturation occurred at 10 µg/ml, whereas the untransfected DCEK.ICAM.Hi7 cell line (Fig. 3 A, none) did not bind HuIgG. Binding required that the human IgG be heat aggregated, as expected for functional FcRIII (Unkeless et al. 1981). On PAGE, <10% of the HuIgG aggregated (molecular mass > 200,000 D) upon heating to 65°C for 30 min (Fig. 3 B), so that saturable binding of the expressed chimeric receptors was occurring at <1 µg/ml of aggHuIgG. When the sensitivity of bound ligand to pH was measured, aggHuIgG began to elute at pH 5, and only 40% remained at pH 4 (Fig. 3 C).
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Similar results, i.e., endocytosis of bound HuIgG, were obtained with cells expressing the LT-, IT-, and AT-DEC:CD16 chimera (not shown). In contrast, cells transfected with the short six–amino acid tail (ST-DEC:CD16), lacking the putative coated pit localization sequence, showed no significant loss of surface IgG (Fig. 3 D), indicating that deletion of amino acid residues 6–31 prevents endocytosis of the DEC:CD16 chimeric receptor. In a more detailed time course study (Fig. 3 E), we included cells expressing AAA-DEC:CD16 and MMR:CD16. Again, the ST-DEC:CD16 chimera did not internalize, whereas half the surface Ig entered the cells via the other CD16 chimeras within 15 min. The AT-DEC:CD16 chimera, which contained a mutation of the tyrosine residue in the coated pit localization site, was capable of endocytosis although at a somewhat slower rate (Fig. 3 E). Therefore, all the chimeric CD16 receptors bound aggHuIgG, and all except the ST-DEC:CD16 mediated rapid adsorptive uptake of ligand.
To examine recycling of the DEC:CD16 chimeric receptors, we blocked protein synthesis using CHX at 10 µg/ml for 2 h. We then added a saturating dose of aggHuIgG, placed the cells on ice, washed, and transferred the L cells to 37°C. This procedure was sufficient to saturate all of the CD16 chimeric receptors (data not shown). Thereafter, at subsequent time points, we added 125I-labeled aggHuIgG to detect a reappearance of functional CD16 receptors. The intact cytosolic tails (WT, MMR) recycled within 1 h after internalization, but the truncated intermediate DEC tail (IT) and the AAA-DEC:CD16 chimera recycled less rapidly (Fig. 3 F). The 1-h recycling time could underestimate the speed of recycling via the DEC-205 tail, because the pH of the vacuolar system in L cells may be insufficiently low to quickly elute all of the bound HuIgG ligand. As expected, the ST tail did not recycle, i.e., regenerate functional CD16, because endocytosis was not occurring. To rule out replenishment of receptors from endocytic pools, rather than recycling, we repeated the experiments in cells exposed for 1 h at 37°C to aggHuIgG to occupy intracellular stores. Again, recycling of new HuIgG binding receptors took place (data not shown). We conclude that the DEC-205 and MMR tails are each capable of mediating ligand uptake, discharge, and recycling to the cell surface, and that these activities can be carried out by all of the mutant cytosolic tails we had prepared, except for the short tail lacking the coated pit localization and uptake sequence.
Intracellular Compartments Targeted by CD16 Chimeric Receptors
At this stage of the studies, the DEC-205 and MMR tails seemed similar. Distinctive features of the DEC-205 tail became apparent upon examining the intracellular targeting of CD16 chimeric receptors and HuIgG ligand, and in tests of antigen presentation to HuIgG-primed T cells. First, we did confocal immunofluorescence microscopy to simultaneously identify MHC II and LAMP-1 or the early endosomal marker TfR. In all transfectants (WT shown here), MHC II largely colocalized with LAMP-1, a marker for late endosomes/lysosomes in the perinuclear region (Fig. 4, top), and not with TfR in the periphery (Fig. 4, bottom).
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Importance of the Distal EDE Sequence in the Distinct Targeting of the DEC-205 Tail
Acidic amino acids are implicated in intracellular targeting, e.g., the movement of HIV-1 nef protein to lysosomes (Piguet et al. 1999), the movement of furin to the trans-Golgi network (Voorhees et al. 1995; Simmen et al. 1999), and the retrieval of the LDLR from apical to basolateral membranes of epithelial cells (Matter et al. 1993). To assess if acidic amino acids in the distal part of the DEC-205 tail were required for late endosomal targeting, we mutated the EDE residues to alanines (AAA-DEC:CD16). The AAA-DEC:CD16 chimera was fully competent for adsorptive uptake of aggHuIgG and recycling back to the surface (Fig. 3E and Fig. F), but failed to target aggHuIgG or CD16 to lysosomes (Fig. 5 B). Consistent with the absence of late endosomal targeting, presentation of antigen to IgG-primed T cells was greatly reduced (Fig. 7 A) and occurred only at antigen levels comparable to those needed for IT-DEC:CD16– and MMR:CD16-mediated presentation. Thus, the EDE in the DEC-205 tail is required for its unique lysosomal targeting and antigen presenting functions.
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Discussion |
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Additional work will be needed to pursue the physiological implication of these findings, i.e., that DEC-205 is used by DCs to improve antigen presentation. The following types of future experiments would be of value. First, to assess the role of the distal cytoplasmic tail in the context of a full length receptor, it would be important to mutate full length DEC-205; currently, it is not yet feasible to obtain high level expression of this large receptor. Second, the studies of CD16-DEC fusion receptors could be extended into DCs using different vectors, and such experiments are underway. Third, ligands for DEC-205 and MMR need to be identified, so that the presentation of natural ligands rather than surrogates can be tested. Interestingly, DEC-205 and not the MMR is readily detected on DCs within the T cell areas of mouse and human lymphoid tissues (Linehan et al. 1999; Guo et al. 2000).
Two Functional Regions of the DEC-205 Cytosolic Domain
The membrane-proximal region of the DEC-205 tail contains the sequence FSSVRY, which resembles the coated pit localization sequences described in many other receptors (Goldstein et al. 1979). Such sequences function in uptake of the LDLR (Chen et al. 1990; Matter et al. 1993), TfR (Collawn et al. 1990), and MMR (Ezekowitz et al. 1990). The DEC-205 tail is 31 residues in length. Deletion of residues 19–31 did not reduce endocytosis, but further deletion of residues 7–19 including the coated pit sequence ablated uptake. Mutation of the tyrosine residue to alanine did not abolish function, in contrast to decreased function of other receptors (Chen et al. 1990; Amigorena et al. 1992b; Jackman et al. 1998), but the uptake rates were lower relative to wild-type DEC-205 tail. Either the coated pit sequence of DEC-205 is not totally dependent on this tyrosine, or another sequence bypasses its need, e.g., the three acidic residues in the distal tail to be discussed next (Voorhees et al. 1995; Simmen et al. 1999).
The more intriguing region of the DEC-205 cytosolic tail was the distal region. Residues 28–31 seemed superfluous, but residues 18–27 were critical for several functions. In the absence of this "distal targeting sequence," the DEC-205 tail did not target either receptor or ligand to late endosomes and lysosomes (Fig. 5) and did not mediate efficient antigen presentation (Fig. 7). However, the distal targeting sequence was not required for uptake (Fig. 3 E) and membrane recycling (Fig. 3 F).
A cluster of acidic amino acids (EDE) in the distal DEC-205 tail proved critical for its distinct intracellular movements (Fig. 5 and Fig. 7). Acidic clusters also mediate trans-Golgi network retrieval of the mannose-6-phosphate receptor and furin, the latter interacting with a cytosolic sorting molecule PACS-1 (Voorhees et al. 1995; Wan et al. 1998; Simmen et al. 1999). Only two acidic amino acids in the HIV-1 nef protein signal lysosomal targeting and degradation of endocytosed CD4 molecules via β-COP (Piguet et al. 1999). Acidic residues target the LDLR to a basolateral site during transcytosis (Matter et al. 1992). The LDLR-related protein contains a cluster of acidic amino acids as well. This receptor mediates uptake and degradation of sphingolipids, 
2-macroglobulin, and complement component C3 into lysosomes (Hiesberger et al. 1998; Meilinger et al. 1999), but the involvement of acidic amino acids in lysosomal targeting has not been described. The experiments in this paper have compared DEC-205 with MMR, because of similarities in their external multilectin domains and their proposed function in antigen presentation. Future work will compare the cytosolic domains of DEC-205 with the LDLR family, as well as function in non–antigen-presenting cells like epithelial cells.
A Novel Pathway for an Adsorptive Endocytosis Receptor
As mentioned above (see Introduction), some receptors mediate ligand uptake and discharge in early endosomes, followed by recycling of intact receptors to the surface and further rounds of uptake. Other receptors signal cell activation and growth, and then uptake is followed by ligand and receptor digestion in lysosomes. The new pathway, illustrated by DEC-205, is a hybrid between these two. The cytosolic domain of this receptor can mediate uptake into deeper vacuoles, followed by recycling of ostensibly intact receptor. Concomitantly, there is a marked improvement in the efficiency with which peptides are salvaged and displayed as MHC–peptide complexes. It will be important now to follow the distribution and function of DEC-205 in epithelia and brain endothelium, where this receptor is also abundant. It is possible that in epithelia, as in antigen-presenting cells, DEC-205 will target to deeper proteolytic vacuoles and lead to the production of biologically active peptides.
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Acknowledgments |
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These experiments were supported by a fellowship from the Deutsche Forschungsgemeinschaft to K. Mahnke (MA 1924/1-1), grants to R.M. Steinman from the Juvenile Diabetes Foundation and the National Institutes of Health (AI13013 and AI39672), to M. Nussenzweig from the Human Science Frontiers Program, and to S. Lee (National Institutes of Health Medical Scientist Training Program grant GM07739).
Submitted: 11 July 2000
Revised: 15 September 2000
Accepted: 15 September 2000
Abbreviations used in this paper: aggHuIgG, aggregated human IgG; BCR, B cell antigen receptor; CHX, cycloheximide; DC, dendritic cell; ICAM, intracellular adhesion molecule; LAMP-1, lysosome-associated membrane protein 1; LDLR, low-density lipoprotein receptor; MMR, macrophage mannose receptor; MHC II, major histocompatibility complex class II; MIIC, MHC II compartment; PLA2R, phospholipase A2 receptor; TfR, transferrin receptor.
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