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© The Rockefeller University Press, 0021-9525/2003/2/464-a $5.00
The Journal of Cell Biology, Volume 160, Number 4, 464-a-465

Rec'd and repaired

The structures of replicating DNAs change (left to right) to allow repair work. Courcelle/AAAS

DNA replication stalls when the polymerase encounters lesions in the DNA, but recovers soon after lesion repair. In a recent work, Justin Courcelle and colleagues (Mississippi State University, Mississippi State, MS) examine what happens to the replication fork during this downtime. The results show that maintaining the correct fork structure depends on recombination proteins that may help to prevent illegitimate strand exchanges.

Courcelle's group used two-dimensional gel electrophoresis to examine the shapes of a replicating bacterial plasmid. Advancing replication forks yielded the expected Y-shaped structure. But UV-induced lesions stalled the replication fork and produced X-shaped structures. These structures represent the nascent DNA backing up from the apex of a Y-shaped fork. The stalled structures were processed by RecQ and RecJ and maintained by RecA and RecF, which are the same proteins that promote homologous DNA pairing during recombinational processes.

A mid-replication stall is "like catching a cell with its pants down," according to Courcelle. "It can't live as one and a half cells for eternity," he says. Unchecked free DNA ends are recombinogenic. Fork stabilization by these Rec proteins may be essential for preventing unwanted mitotic recombination and its potentially cancerous consequences. In addition, fork regression and Rec binding probably delays replication long enough for repair enzymes or SOS polymerases either to repair the lesion or to replicate past it. Whether RecA and RecF recruit repair enzymes or simply maintain an open fork remains to be determined.

Reference:

Courcelle, J., et al. 2003. Science. 10.1126/science.1081328.

Nicole LeBrasseur

lebrasn{at}rockefeller.edu

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This Article Full Text (PDF, 1392K) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by LeBrasseur, N. Search for Related Content PubMed Articles by LeBrasseur, N. Social Bookmarking                            What's this?